##gff-version 3
##sequence-region BBa_K1362205 1 1487
BBa_K1362205	.	tag	13	30	.	+	0	ID=BBa_K1362458;Name=BBa_K1362458
BBa_K1362205	.	polypeptide_domain	1482	1487	.	+	0	ID=BBa_J70594;Name=BBa_J70594
BBa_K1362205	.	stop_codon	1482	1486	.	+	0	ID=annotation2071257;Name=stop;Parent=BBa_J70594
BBa_K1362205	.	polypeptide_domain	31	39	.	+	0	ID=BBa_K1362449;Name=BBa_K1362449
BBa_K1362205	.	sequence_feature	1463	1469	.	+	0	ID=BBa_K1362424;Name=BBa_K1362424
BBa_K1362205	.	polypeptide_domain	367	387	.	+	0	ID=BBa_K1362453;Name=BBa_K1362453
BBa_K1362205	.	engineered_region	394	1462	.	+	0	ID=BBa_J04450;Name=BBa_J04450
BBa_K1362205	.	promoter	394	593	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_J04450
BBa_K1362205	.	non_covalent_binding_site	482	519	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K1362205	.	promoter	530	535	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K1362205	.	promoter	554	559	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K1362205	.	CDS	394	481	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K1362205	.	non_covalent_binding_site	559	593	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K1362205	.	engineered_region	394	593	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K1362205	.	start_codon	566	566	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K1362205	.	terminator	1422	1462	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J04450
BBa_K1362205	.	polyA_site	1449	1462	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1362205	.	stop_codon	1455	1455	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1362205	.	engineered_region	1422	1462	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1362205	.	stem_loop	1429	1448	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1362205	.	CDS	620	1300	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_J04450
BBa_K1362205	.	CDS	620	1294	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K1362205	.	engineered_tag	1301	1325	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K1362205	.	ribosome_entry_site	602	613	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J04450
BBa_K1362205	.	conserved_region	606	609	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1362205	.	terminator	1334	1413	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J04450
BBa_K1362205	.	stem_loop	1345	1388	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1362205	.	engineered_region	1334	1413	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1362205	.	polypeptide_domain	73	366	.	+	0	ID=BBa_K1362429;Name=BBa_K1362429
BBa_K1362205	.	sequence_feature	73	366	.	+	0	ID=annotation2407301;Name=Smt3;Parent=BBa_K1362429
BBa_K1362205	.	polypeptide_domain	1	12	.	+	0	ID=BBa_K1362446;Name=BBa_K1362446
BBa_K1362205	.	polypeptide_domain	40	57	.	+	0	ID=BBa_K1362456;Name=BBa_K1362456
BBa_K1362205	.	polypeptide_domain	1470	1481	.	+	0	ID=BBa_K1362459;Name=BBa_K1362459
BBa_K1362205	.	sequence_feature	388	393	.	+	0	ID=BBa_K1362447;Name=BBa_K1362447
BBa_K1362205	.	polypeptide_domain	58	72	.	+	0	ID=BBa_K1362448;Name=BBa_K1362448
>BBa_K1362205
atgggcagcagccatcatcatcatcatcacagcagcggcctggtgccgcgcggcagccatatggctagcatgtcggact
cagaagtcaatcaagaagctaagccagaggtcaagccagaagtcaagcctgagactcacatcaatttaaaggtgtccga
tggatcttcagagatcttcttcaagatcaaaaagaccactcctttaagaaggctgatggaagcgttcgctaaaagacag
ggtaaggaaatggactccttaagattcttgtacgacggtattagaatccaagctgatcagacccctgaagatttggaca
tggaggataacgatattattgaggctcacagagaacagattggtggatccgaaaacctgtacttccagggtagagacca
atacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgg
gcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcg
tatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggcttcctccg
aagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaagg
tgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgct
tgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctga
aactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccagga
ctcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatg
cagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaa
tgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagct
gccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaa
cgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatca
aataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagt
cacactggctcaccttcgggtgggcctttctgcgtttataggtctcacttccggaaacctaataa