##gff-version 3
##sequence-region BBa_K1362005 1 1522
BBa_K1362005	.	ribosome_entry_site	1	29	.	+	0	ID=BBa_K1362090;Name=BBa_K1362090
BBa_K1362005	.	sequence_feature	21	28	.	+	0	ID=annotation2393796;Name=Shine-Dalgarno;Parent=BBa_K1362090
BBa_K1362005	.	ribosome_entry_site	11	28	.	+	0	ID=annotation2393795;Name=t7 RBS;Parent=BBa_K1362090
BBa_K1362005	.	engineered_region	36	1522	.	+	0	ID=BBa_K1362205;Name=BBa_K1362205
BBa_K1362005	.	polypeptide_domain	1517	1522	.	+	0	ID=BBa_J70594;Name=BBa_J70594;Parent=BBa_K1362205
BBa_K1362005	.	stop_codon	1517	1521	.	+	0	ID=annotation2071257;Name=stop;Parent=BBa_J70594
BBa_K1362005	.	tag	48	65	.	+	0	ID=BBa_K1362458;Name=BBa_K1362458;Parent=BBa_K1362205
BBa_K1362005	.	polypeptide_domain	402	422	.	+	0	ID=BBa_K1362453;Name=BBa_K1362453;Parent=BBa_K1362205
BBa_K1362005	.	sequence_feature	1498	1504	.	+	0	ID=BBa_K1362424;Name=BBa_K1362424;Parent=BBa_K1362205
BBa_K1362005	.	polypeptide_domain	75	92	.	+	0	ID=BBa_K1362456;Name=BBa_K1362456;Parent=BBa_K1362205
BBa_K1362005	.	sequence_feature	423	428	.	+	0	ID=BBa_K1362447;Name=BBa_K1362447;Parent=BBa_K1362205
BBa_K1362005	.	polypeptide_domain	66	74	.	+	0	ID=BBa_K1362449;Name=BBa_K1362449;Parent=BBa_K1362205
BBa_K1362005	.	engineered_region	429	1497	.	+	0	ID=BBa_J04450;Name=BBa_J04450;Parent=BBa_K1362205
BBa_K1362005	.	terminator	1369	1448	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J04450
BBa_K1362005	.	engineered_region	1369	1448	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1362005	.	stem_loop	1380	1423	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1362005	.	promoter	429	628	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_J04450
BBa_K1362005	.	non_covalent_binding_site	517	554	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K1362005	.	promoter	589	594	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K1362005	.	engineered_region	429	628	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K1362005	.	CDS	429	516	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K1362005	.	non_covalent_binding_site	594	628	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K1362005	.	promoter	565	570	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K1362005	.	start_codon	601	601	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K1362005	.	terminator	1457	1497	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J04450
BBa_K1362005	.	engineered_region	1457	1497	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1362005	.	stop_codon	1490	1490	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1362005	.	stem_loop	1464	1483	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1362005	.	polyA_site	1484	1497	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1362005	.	CDS	655	1335	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_J04450
BBa_K1362005	.	engineered_tag	1336	1360	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K1362005	.	CDS	655	1329	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K1362005	.	ribosome_entry_site	637	648	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J04450
BBa_K1362005	.	conserved_region	641	644	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1362005	.	polypeptide_domain	36	47	.	+	0	ID=BBa_K1362446;Name=BBa_K1362446;Parent=BBa_K1362205
BBa_K1362005	.	polypeptide_domain	108	401	.	+	0	ID=BBa_K1362429;Name=BBa_K1362429;Parent=BBa_K1362205
BBa_K1362005	.	sequence_feature	108	401	.	+	0	ID=annotation2407301;Name=Smt3;Parent=BBa_K1362429
BBa_K1362005	.	polypeptide_domain	1505	1516	.	+	0	ID=BBa_K1362459;Name=BBa_K1362459;Parent=BBa_K1362205
BBa_K1362005	.	polypeptide_domain	93	107	.	+	0	ID=BBa_K1362448;Name=BBa_K1362448;Parent=BBa_K1362205
>BBa_K1362005
aataattttgtttaactttaagaaggagatactagatgggcagcagccatcatcatcatcatcacagcagcggcctggt
gccgcgcggcagccatatggctagcatgtcggactcagaagtcaatcaagaagctaagccagaggtcaagccagaagtc
aagcctgagactcacatcaatttaaaggtgtccgatggatcttcagagatcttcttcaagatcaaaaagaccactcctt
taagaaggctgatggaagcgttcgctaaaagacagggtaaggaaatggactccttaagattcttgtacgacggtattag
aatccaagctgatcagacccctgaagatttggacatggaggataacgatattattgaggctcacagagaacagattggt
ggatccgaaaacctgtacttccagggtagagaccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatg
cagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattag
gcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatact
agagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaa
ggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaac
tgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagctta
cgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaac
ttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgc
gtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgta
cccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagtt
aaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctccc
acaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgata
gtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtttta
tctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttataggtc
tcacttccggaaacctaataa