##gff-version 3
##sequence-region BBa_K1362002 1 1192
BBa_K1362002	.	engineered_region	36	1192	.	+	0	ID=BBa_K1362202;Name=BBa_K1362202
BBa_K1362002	.	sequence_feature	1144	1150	.	+	0	ID=BBa_K1362424;Name=BBa_K1362424;Parent=BBa_K1362202
BBa_K1362002	.	engineered_region	75	1143	.	+	0	ID=BBa_J04450;Name=BBa_J04450;Parent=BBa_K1362202
BBa_K1362002	.	terminator	1015	1094	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J04450
BBa_K1362002	.	engineered_region	1015	1094	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1362002	.	stem_loop	1026	1069	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1362002	.	promoter	75	274	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_J04450
BBa_K1362002	.	non_covalent_binding_site	163	200	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K1362002	.	promoter	235	240	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K1362002	.	engineered_region	75	274	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K1362002	.	CDS	75	162	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K1362002	.	non_covalent_binding_site	240	274	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K1362002	.	promoter	211	216	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K1362002	.	start_codon	247	247	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K1362002	.	terminator	1103	1143	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J04450
BBa_K1362002	.	engineered_region	1103	1143	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1362002	.	stop_codon	1136	1136	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1362002	.	stem_loop	1110	1129	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1362002	.	polyA_site	1130	1143	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1362002	.	CDS	301	981	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_J04450
BBa_K1362002	.	engineered_tag	982	1006	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K1362002	.	CDS	301	975	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K1362002	.	ribosome_entry_site	283	294	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J04450
BBa_K1362002	.	conserved_region	287	290	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1362002	.	sequence_feature	69	74	.	+	0	ID=BBa_K1362427;Name=BBa_K1362427;Parent=BBa_K1362202
BBa_K1362002	.	polypeptide_domain	1151	1168	.	+	0	ID=BBa_K1362454;Name=BBa_K1362454;Parent=BBa_K1362202
BBa_K1362002	.	tag	1169	1186	.	+	0	ID=BBa_K1362457;Name=BBa_K1362457;Parent=BBa_K1362202
BBa_K1362002	.	polypeptide_domain	48	68	.	+	0	ID=BBa_K1362452;Name=BBa_K1362452;Parent=BBa_K1362202
BBa_K1362002	.	polypeptide_domain	1187	1192	.	+	0	ID=BBa_J70594;Name=BBa_J70594;Parent=BBa_K1362202
BBa_K1362002	.	stop_codon	1187	1191	.	+	0	ID=annotation2071257;Name=stop;Parent=BBa_J70594
BBa_K1362002	.	polypeptide_domain	36	47	.	+	0	ID=BBa_K1362445;Name=BBa_K1362445;Parent=BBa_K1362202
BBa_K1362002	.	ribosome_entry_site	1	29	.	+	0	ID=BBa_K1362090;Name=BBa_K1362090
BBa_K1362002	.	sequence_feature	21	28	.	+	0	ID=annotation2393796;Name=Shine-Dalgarno;Parent=BBa_K1362090
BBa_K1362002	.	ribosome_entry_site	11	28	.	+	0	ID=annotation2393795;Name=t7 RBS;Parent=BBa_K1362090
>BBa_K1362002
aataattttgtttaactttaagaaggagatactagatgggcagtagtgagaatctttacttccagggttgagaccaata
cgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggca
gtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtat
gttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggcttcctccgaag
acgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtga
aggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgg
gacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaac
tgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactc
ctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcag
aaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgc
gtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgcc
gggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgt
gctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaat
aaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcac
actggctcaccttcgggtgggcctttctgcgtttataggtctcacttcctgaaactggtggtcatcaccatcaccatca
ctaataa