##gff-version 3
##sequence-region BBa_K1321330 1 4718
BBa_K1321330	.	engineered_region	1	1817	.	+	0	ID=BBa_K577895;Name=BBa_K577895
BBa_K1321330	.	ribosome_entry_site	47	58	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K577895
BBa_K1321330	.	conserved_region	51	54	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1321330	.	ribosome_entry_site	957	968	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K577895
BBa_K1321330	.	conserved_region	961	964	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1321330	.	CDS	975	1680	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_K577895
BBa_K1321330	.	engineered_tag	1656	1680	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K1321330	.	CDS	975	1649	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K1321330	.	terminator	1689	1817	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K577895
BBa_K1321330	.	terminator	1689	1768	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1321330	.	stem_loop	1700	1743	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1321330	.	engineered_region	1689	1768	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1321330	.	terminator	1777	1817	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1321330	.	stem_loop	1784	1803	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1321330	.	polyA_site	1804	1817	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1321330	.	engineered_region	1777	1817	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1321330	.	stop_codon	1810	1810	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1321330	.	terminator	758	837	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K577895
BBa_K1321330	.	stem_loop	769	812	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1321330	.	engineered_region	758	837	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1321330	.	promoter	1	38	.	+	0	ID=BBa_I14033;Name=BBa_I14033;Parent=BBa_K577895
BBa_K1321330	.	promoter	4	9	.	+	0	ID=annotation1026172;Name=-35;Parent=BBa_I14033
BBa_K1321330	.	promoter	27	32	.	+	0	ID=annotation1026173;Name=-10;Parent=BBa_I14033
BBa_K1321330	.	operator	1	38	.	+	0	ID=annotation1026174;Name=P(Cat);Parent=BBa_I14033
BBa_K1321330	.	promoter	895	948	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_K577895
BBa_K1321330	.	non_covalent_binding_site	920	938	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K1321330	.	engineered_region	895	948	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K1321330	.	non_covalent_binding_site	895	913	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K1321330	.	promoter	937	942	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K1321330	.	promoter	914	919	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K1321330	.	CDS	65	749	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_K577895
BBa_K1321330	.	tag	685	718	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K1321330	.	engineered_tag	725	749	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K1321330	.	CDS	68	684	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K1321330	.	terminator	846	886	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K577895
BBa_K1321330	.	stem_loop	853	872	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1321330	.	polyA_site	873	886	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1321330	.	engineered_region	846	886	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1321330	.	stop_codon	879	879	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1321330	.	plasmid_vector	1826	4718	.	+	0	ID=BBa_K1321300;Name=BBa_K1321300
BBa_K1321330	.	sequence_feature	3023	3685	.	+	0	ID=annotation2412990;Name=Rep;Parent=BBa_K1321300
BBa_K1321330	.	CDS	2096	2755	.	+	0	ID=annotation2408619;Name=Chloramphenicol acetyltransferase;Parent=BBa_K1321300
BBa_K1321330	.	sequence_feature	2779	3014	.	+	0	ID=annotation2412975;Name=OriT;Parent=BBa_K1321300
BBa_K1321330	.	sequence_feature	1826	1847	.	+	0	ID=annotation2408497;Name=Biobrick suffix;Parent=BBa_K1321300
BBa_K1321330	.	sequence_feature	4697	4718	.	+	0	ID=annotation2408614;Name=Biobrick prefix;Parent=BBa_K1321300
>BBa_K1321330
ggcacgtaagaggttccaactttcaccataatgaaacatactagagaaagaggagaaatactagatgtccagattagat
aaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgccc
agaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattga
gatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaa
agttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatg
aaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgt
ggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactact
gatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggcc
ttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgcttt
agtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcga
aagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgg
gcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
tactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtat
ggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgct
aaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaag
cttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttat
gaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaa
ctgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgta
tgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctga
agttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacc
tcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgct
gatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgt
tttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
tactagagtactagtagcggccgctgcagcttggactcctgttgatagatccagtaatgacctcagaactccatctgga
tttgttcagaacgctcggttgccgccgggcgttttttattggtgagaatccaggggtccccaataattacgatttaaat
tggcgaaaatgagacgttgatcggcacgtaagaggttccaactttcaccataatgaaataagatcactaccgggcgtat
tttttgagttatcgagattttcaggagctaaggaagctaaaatggagaaaaaaatcactggatataccaccgttgatat
atcccaatggcatcgtaaagaacattttgaggcatttcagtcagttgctcaatgtacctataaccagaccgttcagctg
gatattacggcctttttaaagaccgtaaagaaaaataagcacaagttttatccggcctttattcacattcttgcccgcc
tgatgaatgctcatccggaatttcgtatggcaatgaaagacggtgagctggtgatatgggatagtgttcacccttgtta
caccgttttccatgagcaaactgaaacgttttcatcgctctggagtgaataccacgacgatttccggcagtttctacac
atatattcgcaagatgtggcgtgttacggtgaaaacctggcctatttccctaaagggtttattgagaatatgtttttcg
tctcagccaatccctgggtgagtttcaccagttttgatttaaacgtggccaatatggacaacttcttcgcccccgtttt
caccatgggcaaatattatacgcaaggcgacaaggtgctgatgccgctggcgattcaggttcatcatgccgtttgtgat
ggcttccatgtcggcagaatgcttaatgaattacaacagtactgcgatgagtggcagggcggggcgtaatttgactttt
gtccttttccgctgcataaccctgcttcggggtcattatagcgattttttcggtatatccatcctttttcgcacgatat
acaggattttgccaaagggttcgtgtagactttccttggtgtatccaacggcgtcagccgggcaggataggtgaagtag
gcccacccgcgagcgggtgttccttcttcactgtcccttattcgcacctggcggtgctcaacgggaatcctgctctgcg
aggctggccgtaggccggccctaccggcgcggcagcgttacccgtgtcggcggctccaacggctcgccatcgtccagaa
aacacggctcatcgggcatcggcaggcgctgctgcccgcgccgttcccattcctccgtttcggtcaaggctggcaggtc
tggttccatgcccggaatgccgggctggctgggcggctcctcgccggggccggtcggtagttgctgctcgcccggatac
agggtcgggatgcggcgcaggtcgccatgccccaacagcgattcgtcctggtcgtcgtgatcaaccaccacggcggcac
tgaacaccgacaggcgcaactggtcgcggggctggccccacgccacgcggtcattgaccacgtaggccgacacggtgcc
ggggccgttgagcttcacgacggagatccagcgctcggccaccaagtccttgactgcgtattggaccgtccgcaaagaa
cgtccgatgagcttggaaagtgtcttctggctgaccaccacggcgttctggtggcccatctgcgccacgaggtgatgca
gcagcattgccgccgtgggtttcctcgcaataagcccggcccacgcctcatgcgctttgcgttccgtttgcacccagtg
accgggcttgttcttggcttgaatgccgatttctctggactgcgtggccatgcttatctccatgcggtaggggtgccgc
acggttgcggcaccatgcgcaatcagctgcaacttttcggcagcgcgacaacaattatgcgttgcgtaaaagtggcagt
caattacagattttctttaacctacgcaatgagctattgcggggggtgccgcaatgagctgttgcgtaccccccttttt
taagttgttgatttttaagtctttcgcatttcgccctatatctagttctttggtgcccaaagaagggcacccctgcggg
gttcccccacgccttcggcgcggctccccctccggcaaaaagtggcccctccggggcttgttgatcgactgcgcggcct
tcggccttgcccaaggtggcgctgcccccttggaacccccgcactcgccgccgtgaggctcggggggcaggcgggcggg
cttcgcccttcgactgcccccactcgcataggcttgggtcgttccaggcgcgtcaaggccaagccgctgcgcggtcgct
gcgcgagccttgacccgccttccacttggtgtccaaccggcaagcgaagcgcgcaggccgcaggccggaggcttttccc
cagagaaaattaaaaaaattgatggggcaaggccgcaggccgcgcagttggagccggtgggtatgtggtcgaaggctgg
gtagccggtgggcaatccctgtggtcaagctcgtgggcaggcgcagcctgtccatcagcttgtccagcagggttgtcca
cgggccgagcgaagcgagccagccggtggccgctcgcggccatcgtccacatatccacgggctggcaagggagcgcagc
gaccgcgcagggcgaagcccggagagcaagcccgtagggggggcgcgcccagctgtctagggcggcggatttgtcctac
tcaggagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatg
cctttaattaaagcggataacaatttcacacaggagaattcgcggccgcttctagag