##gff-version 3
##sequence-region BBa_K1314014 1 4313
BBa_K1314014	.	sequence_feature	1	176	.	+	0	ID=BBa_K1314012;Name=BBa_K1314012
BBa_K1314014	.	sequence_feature	1	170	.	+	0	ID=annotation2397839;Name=part of nifK;Parent=BBa_K1314012
BBa_K1314014	.	sequence_feature	171	176	.	+	0	ID=annotation2397840;Name=XhoI;Parent=BBa_K1314012
BBa_K1314014	.	engineered_region	185	4313	.	+	0	ID=BBa_K1314008;Name=BBa_K1314008
BBa_K1314014	.	engineered_region	3233	4313	.	+	0	ID=BBa_K1314004;Name=BBa_K1314004;Parent=BBa_K1314008
BBa_K1314014	.	engineered_region	3391	4313	.	+	0	ID=BBa_I13521;Name=BBa_I13521;Parent=BBa_K1314004
BBa_K1314014	.	CDS	3471	4151	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_I13521
BBa_K1314014	.	engineered_tag	4152	4176	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K1314014	.	CDS	3471	4145	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K1314014	.	terminator	4185	4264	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13521
BBa_K1314014	.	engineered_region	4185	4264	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1314014	.	stem_loop	4196	4239	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1314014	.	promoter	3391	3444	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_I13521
BBa_K1314014	.	promoter	3410	3415	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K1314014	.	non_covalent_binding_site	3416	3434	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K1314014	.	engineered_region	3391	3444	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K1314014	.	promoter	3433	3438	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K1314014	.	non_covalent_binding_site	3391	3409	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K1314014	.	ribosome_entry_site	3453	3464	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13521
BBa_K1314014	.	conserved_region	3457	3460	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1314014	.	terminator	4273	4313	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13521
BBa_K1314014	.	engineered_region	4273	4313	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1314014	.	stop_codon	4306	4306	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1314014	.	stem_loop	4280	4299	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1314014	.	polyA_site	4300	4313	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1314014	.	sequence_feature	3233	3382	.	+	0	ID=BBa_K1314002;Name=BBa_K1314002;Parent=BBa_K1314004
BBa_K1314014	.	engineered_region	3233	3382	.	+	0	ID=annotation2397292;Name=random sequence/spacer;Parent=BBa_K1314002
BBa_K1314014	.	engineered_region	185	3224	.	+	0	ID=BBa_K1314006;Name=BBa_K1314006;Parent=BBa_K1314008
BBa_K1314014	.	promoter	185	404	.	+	0	ID=BBa_K1314000;Name=BBa_K1314000;Parent=BBa_K1314006
BBa_K1314014	.	promoter	185	404	.	+	0	ID=annotation2396224;Name=nifH promoter;Parent=BBa_K1314000
BBa_K1314014	.	terminator	3096	3224	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1314006
BBa_K1314014	.	terminator	3096	3175	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1314014	.	engineered_region	3096	3175	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1314014	.	stem_loop	3107	3150	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1314014	.	terminator	3184	3224	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1314014	.	engineered_region	3184	3224	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1314014	.	stop_codon	3217	3217	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1314014	.	stem_loop	3191	3210	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1314014	.	polyA_site	3211	3224	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1314014	.	ribosome_entry_site	413	426	.	+	0	ID=BBa_B0031;Name=BBa_B0031;Parent=BBa_K1314006
BBa_K1314014	.	conserved_region	419	422	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
BBa_K1314014	.	CDS	433	3087	.	+	0	ID=BBa_K145001;Name=BBa_K145001;Parent=BBa_K1314006
BBa_K1314014	.	start_codon	433	435	.	+	0	ID=annotation1968216;Name=start;Parent=BBa_K145001
BBa_K1314014	.	stop_codon	3085	3087	.	+	0	ID=annotation1968218;Name=stop;Parent=BBa_K145001
BBa_K1314014	.	CDS	436	3081	.	+	0	ID=annotation1968243;Name=protein;Parent=BBa_K145001
BBa_K1314014	.	stop_codon	3082	3084	.	+	0	ID=annotation1968217;Name=stop;Parent=BBa_K145001
>BBa_K1314014
gtatcggcttcccgatcttcgaccgtcatcacctgcatcgctccaccaccctgggttacgagggcgccatgcagatcct
gaccaccctggtgaactcgatcctggaacgtctggacgaggaaacccgcggtatgcaggccaccgactacaaccacgac
ctggtacgctaactcgagtactagagtgtcagttttgtcacagggggccggaccaggatggtggacgctcgatggggat
gtcgggccattgttcggttgtagcaattacaacagtcggagtagggggattgtagggggattgttgtgtatcagaccgc
cctgctgctcccgtcgatggataattaatcatttaaaatcaatggtttatttatgtgttgcgggtgctggcacagacgc
tgcattacctactagagtcacacaggaaacctactagatgaacacgattaacatcgctaagaacgacttctctgacatc
gaactggctgctatcccgttcaacactctggctgaccattacggtgagcgtttagctcgcgaacagttggcccttgagc
atgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataa
cgctgccgccaagcctctcatcactaccctactccctaagatgattgcacgcatcaacgactggtttgaggaagtgaaa
gctaagcgcggcaagcgcccgacagccttccagttcctgcaagaaatcaagccggaagccgtagcgtacatcaccatta
agaccactctggcttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaagcgcaatcggtcgggccattga
ggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaaacgttgaggaacaactcaacaag
cgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgacatgctctctaagggtctactcggtggcg
aggcgtggtcttcgtggcataaggaagactctattcatgtaggagtacgctgcatcgagatgctcattgagtcaaccgg
aatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactctgagactatcgaactcgcacctgaatacgct
gaggctatcgcaacccgtgcaggtgcgctggctggcatctctccgatgttccaaccttgcgtagttcctcctaagccgt
ggactggcattactggtggtggctattgggctaacggtcgtcgtcctctggcgctggtgcgtactcacagtaagaaagc
actgatgcgctacgaagacgtttacatgcctgaggtgtacaaagcgattaacattgcgcaaaacaccgcatggaaaatc
aacaagaaagtcctagcggtcgccaacgtaatcaccaagtggaagcattgtccggtcgaggacatccctgcgattgagc
gtgaagaactcccgatgaaaccggaagacatcgacatgaatcctgaggctctcaccgcgtggaaacgtgctgccgctgc
tgtgtaccgcaaggacaaggctcgcaagtctcgccgtatcagccttgagttcatgcttgagcaagccaataagtttgct
aaccataaggccatctggttcccttacaacatggactggcgcggtcgtgtttacgctgtgtcaatgttcaacccgcaag
gtaacgatatgaccaaaggactgcttacgctggcgaaaggtaaaccaatcggtaaggaaggttactactggctgaaaat
ccacggtgcaaactgtgcgggtgtcgataaggttccgttccctgagcgcatcaagttcattgaggaaaaccacgagaac
atcatggcttgcgctaagtctccactggagaacacttggtgggctgagcaagattctccgttctgcttccttgcgttct
gctttgagtacgctggggtacagcaccacggcctgagctataactgctcccttccgctggcgtttgacgggtcttgctc
tggcatccagcacttctccgcgatgctccgagatgaggtaggtggtcgcgcggttaacttgcttcctagtgaaaccgtt
caggacatctacgggattgttgctaagaaagtcaacgagattctacaagcagacgcaatcaatgggaccgataacgaag
tagttaccgtgaccgatgagaacactggtgaaatctctgagaaagtcaagctgggcactaaggcactggctggtcaatg
gctggcttacggtgttactcgcagtgtgactaagcgttcagtcatgacgctggcttacgggtccaaagagttcggcttc
cgtcaacaagtgctggaagataccattcagccagctattgattccggcaagggtctgatgttcactcagccgaatcagg
ctgctggatacatggctaagctgatttgggaatctgtgagcgtgacggtggtagctgcggttgaagcaatgaactggct
taagtctgctgctaagctgctggctgctgaggtcaaagataagaagactggagagattcttcgcaagcgttgcgctgtg
cattgggtaactcctgatggtttccctgtgtggcaggaatacaagaagcctattcagacgcgcttgaacctgatgttcc
tcggtcagttccgcttacagcctaccattaacaccaacaaagatagcgagattgatgcacacaaacaggagtctggtat
cgctcctaactttgtacacagccaagacggtagccaccttcgtaagactgtagtgtgggcacacgagaagtacggaatc
gaatcttttgcactgattcacgactccttcggtaccattccggctgacgctgcgaacctgttcaaagcagtgcgcgaaa
ctatggttgacacatatgagtcttgtgatgtactggctgatttctacgaccagttcgctgaccagttgcacgagtctca
attggacaaaatgccagcacttccggctaaaggtaacttgaacctccgtgacatcttagagtcggacttcgcgttcgcg
taataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttg
tcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagggaattg
gggcccttgtggcctaaatccaaccgcggatcagtcagggcgcggatcccctgcaacgtcgcgacgctagagatactct
atccagcggtagctcgtgtggttcaaagggtaagccgtcacgctggcttcggaggtctatctgctactagagtccctat
cagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatggct
tcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaa
tcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgcc
gttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggac
tacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgtta
cccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtcc
ggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaa
atcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccgg
ttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaaca
gtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagcca
ggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcta
ctagagtcacactggctcaccttcgggtgggcctttctgcgtttata