##gff-version 3
##sequence-region BBa_K1314007 1 4127
BBa_K1314007	.	engineered_region	3047	4127	.	+	0	ID=BBa_K1314004;Name=BBa_K1314004
BBa_K1314007	.	engineered_region	3205	4127	.	+	0	ID=BBa_I13521;Name=BBa_I13521;Parent=BBa_K1314004
BBa_K1314007	.	ribosome_entry_site	3267	3278	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13521
BBa_K1314007	.	conserved_region	3271	3274	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1314007	.	terminator	3999	4078	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13521
BBa_K1314007	.	stem_loop	4010	4053	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1314007	.	engineered_region	3999	4078	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1314007	.	CDS	3285	3965	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_I13521
BBa_K1314007	.	CDS	3285	3959	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K1314007	.	engineered_tag	3966	3990	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K1314007	.	terminator	4087	4127	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13521
BBa_K1314007	.	polyA_site	4114	4127	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1314007	.	stop_codon	4120	4120	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1314007	.	stem_loop	4094	4113	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1314007	.	engineered_region	4087	4127	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1314007	.	promoter	3205	3258	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_I13521
BBa_K1314007	.	non_covalent_binding_site	3205	3223	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K1314007	.	promoter	3224	3229	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K1314007	.	non_covalent_binding_site	3230	3248	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K1314007	.	engineered_region	3205	3258	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K1314007	.	promoter	3247	3252	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K1314007	.	sequence_feature	3047	3196	.	+	0	ID=BBa_K1314002;Name=BBa_K1314002;Parent=BBa_K1314004
BBa_K1314007	.	engineered_region	3047	3196	.	+	0	ID=annotation2397292;Name=random sequence/spacer;Parent=BBa_K1314002
BBa_K1314007	.	engineered_region	1	3038	.	+	0	ID=BBa_K1314005;Name=BBa_K1314005
BBa_K1314007	.	CDS	247	2901	.	+	0	ID=BBa_K145001;Name=BBa_K145001;Parent=BBa_K1314005
BBa_K1314007	.	stop_codon	2896	2898	.	+	0	ID=annotation1968217;Name=stop;Parent=BBa_K145001
BBa_K1314007	.	stop_codon	2899	2901	.	+	0	ID=annotation1968218;Name=stop;Parent=BBa_K145001
BBa_K1314007	.	start_codon	247	249	.	+	0	ID=annotation1968216;Name=start;Parent=BBa_K145001
BBa_K1314007	.	CDS	250	2895	.	+	0	ID=annotation1968243;Name=protein;Parent=BBa_K145001
BBa_K1314007	.	ribosome_entry_site	229	240	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K1314005
BBa_K1314007	.	conserved_region	233	236	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1314007	.	promoter	1	220	.	+	0	ID=BBa_K1314000;Name=BBa_K1314000;Parent=BBa_K1314005
BBa_K1314007	.	promoter	1	220	.	+	0	ID=annotation2396224;Name=nifH promoter;Parent=BBa_K1314000
BBa_K1314007	.	terminator	2910	3038	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1314005
BBa_K1314007	.	terminator	2998	3038	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1314007	.	polyA_site	3025	3038	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1314007	.	stop_codon	3031	3031	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1314007	.	stem_loop	3005	3024	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1314007	.	engineered_region	2998	3038	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1314007	.	terminator	2910	2989	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1314007	.	stem_loop	2921	2964	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1314007	.	engineered_region	2910	2989	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
>BBa_K1314007
tgtcagttttgtcacagggggccggaccaggatggtggacgctcgatggggatgtcgggccattgttcggttgtagcaa
ttacaacagtcggagtagggggattgtagggggattgttgtgtatcagaccgccctgctgctcccgtcgatggataatt
aatcatttaaaatcaatggtttatttatgtgttgcgggtgctggcacagacgctgcattacctactagagaaagaggag
aaatactagatgaacacgattaacatcgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactc
tggctgaccattacggtgagcgtttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacg
cttccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactacc
ctactccctaagatgattgcacgcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagcct
tccagttcctgcaagaaatcaagccggaagccgtagcgtacatcaccattaagaccactctggcttgcctaaccagtgc
tgacaatacaaccgttcaggctgtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgt
gaccttgaagctaagcacttcaagaaaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcat
ttatgcaagttgtcgaggctgacatgctctctaagggtctactcggtggcgaggcgtggtcttcgtggcataaggaaga
ctctattcatgtaggagtacgctgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgct
ggcgtagtaggtcaagactctgagactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggtgcgc
tggctggcatctctccgatgttccaaccttgcgtagttcctcctaagccgtggactggcattactggtggtggctattg
ggctaacggtcgtcgtcctctggcgctggtgcgtactcacagtaagaaagcactgatgcgctacgaagacgtttacatg
cctgaggtgtacaaagcgattaacattgcgcaaaacaccgcatggaaaatcaacaagaaagtcctagcggtcgccaacg
taatcaccaagtggaagcattgtccggtcgaggacatccctgcgattgagcgtgaagaactcccgatgaaaccggaaga
catcgacatgaatcctgaggctctcaccgcgtggaaacgtgctgccgctgctgtgtaccgcaaggacaaggctcgcaag
tctcgccgtatcagccttgagttcatgcttgagcaagccaataagtttgctaaccataaggccatctggttcccttaca
acatggactggcgcggtcgtgtttacgctgtgtcaatgttcaacccgcaaggtaacgatatgaccaaaggactgcttac
gctggcgaaaggtaaaccaatcggtaaggaaggttactactggctgaaaatccacggtgcaaactgtgcgggtgtcgat
aaggttccgttccctgagcgcatcaagttcattgaggaaaaccacgagaacatcatggcttgcgctaagtctccactgg
agaacacttggtgggctgagcaagattctccgttctgcttccttgcgttctgctttgagtacgctggggtacagcacca
cggcctgagctataactgctcccttccgctggcgtttgacgggtcttgctctggcatccagcacttctccgcgatgctc
cgagatgaggtaggtggtcgcgcggttaacttgcttcctagtgaaaccgttcaggacatctacgggattgttgctaaga
aagtcaacgagattctacaagcagacgcaatcaatgggaccgataacgaagtagttaccgtgaccgatgagaacactgg
tgaaatctctgagaaagtcaagctgggcactaaggcactggctggtcaatggctggcttacggtgttactcgcagtgtg
actaagcgttcagtcatgacgctggcttacgggtccaaagagttcggcttccgtcaacaagtgctggaagataccattc
agccagctattgattccggcaagggtctgatgttcactcagccgaatcaggctgctggatacatggctaagctgatttg
ggaatctgtgagcgtgacggtggtagctgcggttgaagcaatgaactggcttaagtctgctgctaagctgctggctgct
gaggtcaaagataagaagactggagagattcttcgcaagcgttgcgctgtgcattgggtaactcctgatggtttccctg
tgtggcaggaatacaagaagcctattcagacgcgcttgaacctgatgttcctcggtcagttccgcttacagcctaccat
taacaccaacaaagatagcgagattgatgcacacaaacaggagtctggtatcgctcctaactttgtacacagccaagac
ggtagccaccttcgtaagactgtagtgtgggcacacgagaagtacggaatcgaatcttttgcactgattcacgactcct
tcggtaccattccggctgacgctgcgaacctgttcaaagcagtgcgcgaaactatggttgacacatatgagtcttgtga
tgtactggctgatttctacgaccagttcgctgaccagttgcacgagtctcaattggacaaaatgccagcacttccggct
aaaggtaacttgaacctccgtgacatcttagagtcggacttcgcgttcgcgtaataatactagagccaggcatcaaata
aaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcaca
ctggctcaccttcgggtgggcctttctgcgtttatatactagagggaattggggcccttgtggcctaaatccaaccgcg
gatcagtcagggcgcggatcccctgcaacgtcgcgacgctagagatactctatccagcggtagctcgtgtggttcaaag
ggtaagccgtcacgctggcttcggaggtctatctgctactagagtccctatcagtgatagagattgacatccctatcag
tgatagagatactgagcactactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttca
tgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgta
cgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcag
ttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtt
tcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtga
gttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgg
gaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacg
gtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccga
catcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactcc
accggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtc
gaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggt
gggcctttctgcgtttata