##gff-version 3
##sequence-region BBa_K1172405 1 5150
BBa_K1172405	.	promoter	1	32	.	+	0	ID=BBa_K525998;Name=BBa_K525998
BBa_K1172405	.	ribosome_entry_site	24	27	.	+	0	ID=annotation2129433;Name=RBS;Parent=BBa_K525998
BBa_K1172405	.	promoter	1	20	.	+	0	ID=annotation2129432;Name=T7 promoter;Parent=BBa_K525998
BBa_K1172405	.	engineered_region	21	32	.	+	0	ID=annotation2127788;Name=B0034;Parent=BBa_K525998
BBa_K1172405	.	CDS	39	5150	.	+	0	ID=BBa_K1172401;Name=BBa_K1172401
BBa_K1172405	.	point_mutation	1836	1836	.	+	0	ID=annotation2370539;Name=PstI site G->A;Parent=BBa_K1172401
BBa_K1172405	.	CDS	39	2054	.	+	0	ID=annotation2370537;Name=mtrC;Parent=BBa_K1172401
BBa_K1172405	.	start_codon	2055	2057	.	+	0	ID=annotation2370543;Name=start;Parent=BBa_K1172401
BBa_K1172405	.	CDS	3057	5150	.	+	0	ID=annotation2370542;Name=mtrB;Parent=BBa_K1172401
BBa_K1172405	.	point_mutation	2121	2121	.	+	0	ID=annotation2370541;Name=pstI site G->A;Parent=BBa_K1172401
BBa_K1172405	.	start_codon	3057	3059	.	+	0	ID=annotation2370544;Name=start;Parent=BBa_K1172401
BBa_K1172405	.	CDS	2055	3056	.	+	0	ID=annotation2370540;Name=mtrA;Parent=BBa_K1172401
BBa_K1172405	.	start_codon	39	44	.	+	0	ID=annotation2370538;Name=start;Parent=BBa_K1172401
>BBa_K1172405
taatacgactcactatagggaaagaggagaaatactagatgatgaacgcacaaaaatcaaaaatcgcactgctgctcgc
agcaagtgccgtcacaatggccttaaccggctgtggtggaagcgatggtaataacggcaatgatggtagtgatggtggt
gagccagcaggtagcatccagacgttaaacctagatatcactaaagtaagctatgaaaatggtgcacctatggtcactg
ttttcgccactaacgaagccgacatgccagtgattggtctcgcaaatttagaaatcaaaaaagcactgcaattaatacc
ggaaggggcgacaggcccaggtaatagcgctaactggcaaggcttaggctcatcaaagagctatgtcgataataaaaac
ggtagctatacctttaaattcgacgccttcgatagtaataaggtctttaatgctcaattaacgcaacgctttaacgttg
tttctgctgcgggtaaattagcagacggaacgaccgttcccgttgccgaaatggttgaagatttcgacggccaaggtaa
tgcgccgcaatatacaaaaaatatcgttagccacgaagtatgtgcttcttgccacgtagaaggtgaaaagatttatcac
caagctactgaagtcgaaacttgtatttcttgccacactcaagagtttgcggatggtcgcggcaaaccccatgtcgcct
ttagtcacttaattcacaatgtgcataatgccaacaaagcttggggcaaagacaataaaatccctacagttgcacaaaa
tattgtccaagataattgccaagtttgtcacgttgaatccgacatgctcaccgaggcaaaaaactggtcacgtattcca
acaatggaagtctgttctagctgtcacgtagacatcgattttgctgcgggtaaaggccactctcaacaactcgataact
ccaactgtatcgcctgccataacagcgactggactgctgagttacacacagccaaaaccaccgcaactaagaacttgat
taatcaatacggtatcgagactacctcgacaattaataccgaaactaaagcagccacaattagtgttcaagttgtagat
gcgaacggtactgctgttgatctcaagaccatcctgcctaaagtgcaacgcttagagatcatcaccaacgttggtccta
ataatgcaaccttaggttatagtggcaaagattcaatatttgcaatcaaaaatggagctcttgatccaaaagctactat
caatgatgctggcaaactggtttataccactactaaagacctcaaacttggccaaaacggcgcagacagcgacacagca
tttagctttgtaggttggtcaatgtgttctagcgaaggtaagtttgtagactgtgcagaccctgcatttgatggtgttg
atgtaactaagtataccggcatgaaagcggatttagcctttgctactttgtcaggtaaagcaccaagtactcgccacgt
tgattctgttaacatgacagcctgtgccaattgccacactgctgagttcgaaattcacaaaggcaaacaacatgcaggc
tttgtgatgacagagcaactatcacacacccaagatgctaacggtaaagcgattgtaggccttgacgcatgtgtgactt
gtcatactcctgatggcacctatagctttgccaaccgtggtgcgctagagctaaaactacacaaaaaacacgttgaaga
tgcctacggcctcattggtggcaattgtgcctcttgtcactcagacttcaaccttgagtctttcaagaagaaaggcgca
ttgaatactgccgctgcaacagataaaacaggtctatattctacgccgatcactgcaacttgtactacctgtcacacag
ttggcagccagtacatggtccatacgaaagaaaccctggagtctttcggtgcagttgttgatggcacaaaagatgatgc
taccagtgcggcacagtcagaaacctgtttctactgccataccccaacagttgcagatcacactaaagtgaaaatgtaa
atgaagaactgcctaaaaatgaaaaacctactgccggcacttaccatcacaatggcaatgtctgcaattatggcattag
tcgtcacaccaaacgcttatgcgtcgaagtgggatgagaaaatgacgccagagcaagtcgaagccaccttagataagaa
gtttgccgaaggcaactactcccctaaaggcgccgattcttgcttgatgtgccataagaaatccgaaaaagtcatggac
cttttcaaaggtgtccacggtgcgattgactcctctaagagtccaatggctggcctgcaatgtgaggcatgccacggcc
cactgggtcagcacaacaaaggcggcaacgagccgatgatcacttttggtaagcaatcaaccttaagtgccgacaagca
aaacagcgtatgtatgagctgtcaccaagacgataagcgtatgtcttggaatggcggtcaccatgacaatgccgatgtt
gcttgtgcttcttgtcaccaagtacacgtcgcaaaagatcctgtgttatctaaaaacacggaaatggaagtctgtacta
gctgccatacaaagcaaaaagcggatatgaataaacgctcaagtcacccactcaaatgggcacaaatgacctgtagcga
ctgtcacaatccccatgggagcatgacagattccgatcttaacaagcctagcgtgaatgatacctgttattcctgtcac
gccgaaaaacgcggcccaaaactttgggagcatgcacccgtcactgagaattgtgtcacttgccacaatcctcacggta
gtgtgaatgacggtatgctgaaaacccgtgcgccacagctatgtcagcaatgtcacgccagcgatggccacgccagcaa
cgcctacttaggtaacactggattaggttcaaatgtcggtgacaatgcctttactggtggaagaagctgcttaaattgc
catagtcaggttcatggttctaaccatccatctggcaagctattacagcgctaaatgaaatttaaactcaatttgatca
ctctagcgttattagccaacacaggcttggccgtcgctgctgatggttatggtctagcgaatgccaatactgaaaaagt
gaaattatccgcatggagctgtaaaggctgcgtcgttgaaacgggcacatcaggcactgtgggtgtcggtgtcggttat
aacagcgaagaggatattcgctctgccaatgcctttggtacatccaatgaagtggcgggtaaatttgatgccgatttaa
actttaaaggtgaaaagggttatcgtgccagtgttgatgcttatcaactcggtatggatggcggtcgcttagatgtcaa
tgcgggcaaacaaggccagtacaacgtcaatgtgaactatcgccaaattgctacctacgacagcaatagcgccctatcg
ccctacgcgggtattggtggcaataacctcacgttaccggataactggataacagcaggttcaagcaaccaaatgccac
tcttgatggacagcctcaatgccctcgaactctcacttaaacgtgagcgcacggggttgggatttgaatatcaaggtga
atccctgtggagcacctatgttaactacatgcgtgaagagaaaaccggcttaaaacaagcctctggtagcttcttcaac
caatcgatgatgttagcagagccggtggattacaccactgacaccattgaagcgggtgtcaaactcaagggtgatcgtt
ggtttaccgcactcagttacaatgggtcaatattcaaaaacgaatacaaccaattggactttgaaaatgcttttaaccc
cacctttggtgctcaaacccaaggtacgatggcactcgatccggataaccagtcacacaccgtgtcgctgatgggacag
tacaacgatggcagcaacgcactgtcgggtcgtattctgaccggacaaatgagccaagatcaggcgttagtgacggata
actaccgttatgctaatcagctcaataccgatgccgtcgatgccaaagtcgatctactgggtatgaacctgaaagtcgt
tagcaaagtgagcaatgatcttcgcttaacaggtagttacgattattacgaccgtgacaataatacccaagtagaagaa
tggactcagatcagcatcaacaatgtcaacggtaaggtggcttataacaccccttacgataatcgtacgcaacgcttta
aagttgccgcagattatcgcattacccgcgatatcaaactcgatggtggttatgacttcaaacgtgaccaacgtgatta
tcaagaccgtgaaaccacggatgaaaataccgtttgggcccgtttacgtgtaaacagcttcgatacttgggacatgtgg
gtaaaaggcagttacggtaaccgtgacggctcacaataccaagcgtctgaatggacctcttctgaaaccaacagcctgt
tacgtaagtacaatctggctgaccgtgacagaactcaagtcgaagcacggatcacccattcgccattagaaagcctgac
tatcgatgttggtgcccgttacgcgttagatgattataccgatactgtgattggattaactgagtcaaaagacaccagt
tatgatgccaacatcagttatatgatcaccgctgacttactggcaaccgccttctacaattaccaaaccattgagtctg
aacaggcgggtagcagcaattacagcaccccaacgtggacaggctttatagaagatcaggtagatgtggtcggtgcagg
tatcagctacaacaatctgctggagaacaagttacgcctaggactggactacacctattccaactccgacagtaacact
caagtcagacaaggtatcactggcgactatggtgattattttgccaaagtgcataacattaacttatacgctcaatatc
aagccaccgagaaactcgcgctgcgcttcgattacaaaattgagaactataaggacaatgacgccgcaaatgatatcgc
cgttgatggcatttggaacgtcgtaggttttggtagtaacagccatgactacaccgcacaaatgctgatgctgagcatg
agttacaaactctaa