##gff-version 3
##sequence-region BBa_K1172403 1 5174
BBa_K1172403	.	engineered_region	1	56	.	+	0	ID=BBa_K608006;Name=BBa_K608006
BBa_K1172403	.	promoter	1	35	.	+	0	ID=BBa_J23110;Name=BBa_J23110;Parent=BBa_K608006
BBa_K1172403	.	ribosome_entry_site	44	56	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K608006
BBa_K1172403	.	engineered_region	44	56	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K1172403	.	ribosome_entry_site	44	56	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K1172403	.	ribosome_entry_site	50	53	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K1172403	.	CDS	63	5174	.	+	0	ID=BBa_K1172401;Name=BBa_K1172401
BBa_K1172403	.	point_mutation	1860	1860	.	+	0	ID=annotation2370539;Name=PstI site G->A;Parent=BBa_K1172401
BBa_K1172403	.	CDS	3081	5174	.	+	0	ID=annotation2370542;Name=mtrB;Parent=BBa_K1172401
BBa_K1172403	.	CDS	63	2078	.	+	0	ID=annotation2370537;Name=mtrC;Parent=BBa_K1172401
BBa_K1172403	.	start_codon	2079	2081	.	+	0	ID=annotation2370543;Name=start;Parent=BBa_K1172401
BBa_K1172403	.	CDS	2079	3080	.	+	0	ID=annotation2370540;Name=mtrA;Parent=BBa_K1172401
BBa_K1172403	.	point_mutation	2145	2145	.	+	0	ID=annotation2370541;Name=pstI site G->A;Parent=BBa_K1172401
BBa_K1172403	.	start_codon	3081	3083	.	+	0	ID=annotation2370544;Name=start;Parent=BBa_K1172401
BBa_K1172403	.	start_codon	63	68	.	+	0	ID=annotation2370538;Name=start;Parent=BBa_K1172401
>BBa_K1172403
tttacggctagctcagtcctaggtacaatgctagctactagagtcacacaggaaagtactagatgatgaacgcacaaaa
atcaaaaatcgcactgctgctcgcagcaagtgccgtcacaatggccttaaccggctgtggtggaagcgatggtaataac
ggcaatgatggtagtgatggtggtgagccagcaggtagcatccagacgttaaacctagatatcactaaagtaagctatg
aaaatggtgcacctatggtcactgttttcgccactaacgaagccgacatgccagtgattggtctcgcaaatttagaaat
caaaaaagcactgcaattaataccggaaggggcgacaggcccaggtaatagcgctaactggcaaggcttaggctcatca
aagagctatgtcgataataaaaacggtagctatacctttaaattcgacgccttcgatagtaataaggtctttaatgctc
aattaacgcaacgctttaacgttgtttctgctgcgggtaaattagcagacggaacgaccgttcccgttgccgaaatggt
tgaagatttcgacggccaaggtaatgcgccgcaatatacaaaaaatatcgttagccacgaagtatgtgcttcttgccac
gtagaaggtgaaaagatttatcaccaagctactgaagtcgaaacttgtatttcttgccacactcaagagtttgcggatg
gtcgcggcaaaccccatgtcgcctttagtcacttaattcacaatgtgcataatgccaacaaagcttggggcaaagacaa
taaaatccctacagttgcacaaaatattgtccaagataattgccaagtttgtcacgttgaatccgacatgctcaccgag
gcaaaaaactggtcacgtattccaacaatggaagtctgttctagctgtcacgtagacatcgattttgctgcgggtaaag
gccactctcaacaactcgataactccaactgtatcgcctgccataacagcgactggactgctgagttacacacagccaa
aaccaccgcaactaagaacttgattaatcaatacggtatcgagactacctcgacaattaataccgaaactaaagcagcc
acaattagtgttcaagttgtagatgcgaacggtactgctgttgatctcaagaccatcctgcctaaagtgcaacgcttag
agatcatcaccaacgttggtcctaataatgcaaccttaggttatagtggcaaagattcaatatttgcaatcaaaaatgg
agctcttgatccaaaagctactatcaatgatgctggcaaactggtttataccactactaaagacctcaaacttggccaa
aacggcgcagacagcgacacagcatttagctttgtaggttggtcaatgtgttctagcgaaggtaagtttgtagactgtg
cagaccctgcatttgatggtgttgatgtaactaagtataccggcatgaaagcggatttagcctttgctactttgtcagg
taaagcaccaagtactcgccacgttgattctgttaacatgacagcctgtgccaattgccacactgctgagttcgaaatt
cacaaaggcaaacaacatgcaggctttgtgatgacagagcaactatcacacacccaagatgctaacggtaaagcgattg
taggccttgacgcatgtgtgacttgtcatactcctgatggcacctatagctttgccaaccgtggtgcgctagagctaaa
actacacaaaaaacacgttgaagatgcctacggcctcattggtggcaattgtgcctcttgtcactcagacttcaacctt
gagtctttcaagaagaaaggcgcattgaatactgccgctgcaacagataaaacaggtctatattctacgccgatcactg
caacttgtactacctgtcacacagttggcagccagtacatggtccatacgaaagaaaccctggagtctttcggtgcagt
tgttgatggcacaaaagatgatgctaccagtgcggcacagtcagaaacctgtttctactgccataccccaacagttgca
gatcacactaaagtgaaaatgtaaatgaagaactgcctaaaaatgaaaaacctactgccggcacttaccatcacaatgg
caatgtctgcaattatggcattagtcgtcacaccaaacgcttatgcgtcgaagtgggatgagaaaatgacgccagagca
agtcgaagccaccttagataagaagtttgccgaaggcaactactcccctaaaggcgccgattcttgcttgatgtgccat
aagaaatccgaaaaagtcatggaccttttcaaaggtgtccacggtgcgattgactcctctaagagtccaatggctggcc
tgcaatgtgaggcatgccacggcccactgggtcagcacaacaaaggcggcaacgagccgatgatcacttttggtaagca
atcaaccttaagtgccgacaagcaaaacagcgtatgtatgagctgtcaccaagacgataagcgtatgtcttggaatggc
ggtcaccatgacaatgccgatgttgcttgtgcttcttgtcaccaagtacacgtcgcaaaagatcctgtgttatctaaaa
acacggaaatggaagtctgtactagctgccatacaaagcaaaaagcggatatgaataaacgctcaagtcacccactcaa
atgggcacaaatgacctgtagcgactgtcacaatccccatgggagcatgacagattccgatcttaacaagcctagcgtg
aatgatacctgttattcctgtcacgccgaaaaacgcggcccaaaactttgggagcatgcacccgtcactgagaattgtg
tcacttgccacaatcctcacggtagtgtgaatgacggtatgctgaaaacccgtgcgccacagctatgtcagcaatgtca
cgccagcgatggccacgccagcaacgcctacttaggtaacactggattaggttcaaatgtcggtgacaatgcctttact
ggtggaagaagctgcttaaattgccatagtcaggttcatggttctaaccatccatctggcaagctattacagcgctaaa
tgaaatttaaactcaatttgatcactctagcgttattagccaacacaggcttggccgtcgctgctgatggttatggtct
agcgaatgccaatactgaaaaagtgaaattatccgcatggagctgtaaaggctgcgtcgttgaaacgggcacatcaggc
actgtgggtgtcggtgtcggttataacagcgaagaggatattcgctctgccaatgcctttggtacatccaatgaagtgg
cgggtaaatttgatgccgatttaaactttaaaggtgaaaagggttatcgtgccagtgttgatgcttatcaactcggtat
ggatggcggtcgcttagatgtcaatgcgggcaaacaaggccagtacaacgtcaatgtgaactatcgccaaattgctacc
tacgacagcaatagcgccctatcgccctacgcgggtattggtggcaataacctcacgttaccggataactggataacag
caggttcaagcaaccaaatgccactcttgatggacagcctcaatgccctcgaactctcacttaaacgtgagcgcacggg
gttgggatttgaatatcaaggtgaatccctgtggagcacctatgttaactacatgcgtgaagagaaaaccggcttaaaa
caagcctctggtagcttcttcaaccaatcgatgatgttagcagagccggtggattacaccactgacaccattgaagcgg
gtgtcaaactcaagggtgatcgttggtttaccgcactcagttacaatgggtcaatattcaaaaacgaatacaaccaatt
ggactttgaaaatgcttttaaccccacctttggtgctcaaacccaaggtacgatggcactcgatccggataaccagtca
cacaccgtgtcgctgatgggacagtacaacgatggcagcaacgcactgtcgggtcgtattctgaccggacaaatgagcc
aagatcaggcgttagtgacggataactaccgttatgctaatcagctcaataccgatgccgtcgatgccaaagtcgatct
actgggtatgaacctgaaagtcgttagcaaagtgagcaatgatcttcgcttaacaggtagttacgattattacgaccgt
gacaataatacccaagtagaagaatggactcagatcagcatcaacaatgtcaacggtaaggtggcttataacacccctt
acgataatcgtacgcaacgctttaaagttgccgcagattatcgcattacccgcgatatcaaactcgatggtggttatga
cttcaaacgtgaccaacgtgattatcaagaccgtgaaaccacggatgaaaataccgtttgggcccgtttacgtgtaaac
agcttcgatacttgggacatgtgggtaaaaggcagttacggtaaccgtgacggctcacaataccaagcgtctgaatgga
cctcttctgaaaccaacagcctgttacgtaagtacaatctggctgaccgtgacagaactcaagtcgaagcacggatcac
ccattcgccattagaaagcctgactatcgatgttggtgcccgttacgcgttagatgattataccgatactgtgattgga
ttaactgagtcaaaagacaccagttatgatgccaacatcagttatatgatcaccgctgacttactggcaaccgccttct
acaattaccaaaccattgagtctgaacaggcgggtagcagcaattacagcaccccaacgtggacaggctttatagaaga
tcaggtagatgtggtcggtgcaggtatcagctacaacaatctgctggagaacaagttacgcctaggactggactacacc
tattccaactccgacagtaacactcaagtcagacaaggtatcactggcgactatggtgattattttgccaaagtgcata
acattaacttatacgctcaatatcaagccaccgagaaactcgcgctgcgcttcgattacaaaattgagaactataagga
caatgacgccgcaaatgatatcgccgttgatggcatttggaacgtcgtaggttttggtagtaacagccatgactacacc
gcacaaatgctgatgctgagcatgagttacaaactctaa