##gff-version 3
##sequence-region BBa_K1172305 1 3659
BBa_K1172305	.	engineered_region	1	56	.	+	0	ID=BBa_K608006;Name=BBa_K608006
BBa_K1172305	.	promoter	1	35	.	+	0	ID=BBa_J23110;Name=BBa_J23110;Parent=BBa_K608006
BBa_K1172305	.	ribosome_entry_site	44	56	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K608006
BBa_K1172305	.	engineered_region	44	56	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K1172305	.	ribosome_entry_site	44	56	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K1172305	.	ribosome_entry_site	50	53	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K1172305	.	CDS	63	3659	.	+	0	ID=BBa_K1172303;Name=BBa_K1172303
BBa_K1172305	.	stop_codon	1870	1872	.	+	0	ID=annotation2370722;Name=tag;Parent=BBa_K1172303
BBa_K1172305	.	point_mutation	296	301	.	+	0	ID=annotation2370714;Name=Pst1 C->T;Parent=BBa_K1172303
BBa_K1172305	.	start_codon	3183	3185	.	+	0	ID=annotation2370720;Name=atg;Parent=BBa_K1172303
BBa_K1172305	.	stop_codon	3040	3042	.	+	0	ID=annotation2370723;Name=taa;Parent=BBa_K1172303
BBa_K1172305	.	CDS	1216	1872	.	+	0	ID=annotation2370311;Name=SO_3468;Parent=BBa_K1172303
BBa_K1172305	.	point_mutation	1706	1711	.	+	0	ID=annotation2370715;Name=Pst1 C->T;Parent=BBa_K1172303
BBa_K1172305	.	stop_codon	1206	1208	.	+	0	ID=annotation2370721;Name=tga;Parent=BBa_K1172303
BBa_K1172305	.	CDS	1939	3042	.	+	0	ID=annotation2370312;Name=ribBA;Parent=BBa_K1172303
BBa_K1172305	.	CDS	3183	3659	.	+	0	ID=annotation2370313;Name=ribE;Parent=BBa_K1172303
BBa_K1172305	.	point_mutation	1902	1907	.	+	0	ID=annotation2370716;Name=EcoR1 C->T;Parent=BBa_K1172303
BBa_K1172305	.	stop_codon	3657	3659	.	+	0	ID=annotation2370724;Name=taa;Parent=BBa_K1172303
BBa_K1172305	.	start_codon	1216	1218	.	+	0	ID=annotation2370718;Name=atg;Parent=BBa_K1172303
BBa_K1172305	.	start_codon	63	65	.	+	0	ID=annotation2370717;Name=atg;Parent=BBa_K1172303
BBa_K1172305	.	CDS	63	1208	.	+	0	ID=annotation2370310;Name=ribD;Parent=BBa_K1172303
BBa_K1172305	.	start_codon	1939	1941	.	+	0	ID=annotation2370719;Name=atg;Parent=BBa_K1172303
>BBa_K1172305
tttacggctagctcagtcctaggtacaatgctagctactagagtcacacaggaaagtactagatgaattggtcagtact
cgataaccaaatgatgagccgagctattcaattggctcgcaaaggattttataccactcgccccaatcccagtgtgggc
tgcgttatcgttaacgataaccagattgtcggtgaaggttatcatcaaaaagccggagagccccacgctgaagtgcatg
cgctgcgcatggctggcgagcttgcccgtggcgcgactgcttatgtcaccttagaaccctgtagccattatggtcgtac
gccgccctgcgccttggcgctgatcaatattggtgtaaagcgggtggtggtggcggttgaagatcctaatccgcaggtc
ggcgggcgcggtattcaaatgctgcgcgatgcgggtattcaagtggatgtgggcttgcatcgtgatgaggcctatgcct
taaatcttggttttatgaagcgcatggagtcgggtctaccttgggtgacggtaaagcttgccgcgagtcttgatggtaa
aactgcgttatcaaacggggtgtctaaatggatcactggccctgaagctcgccgcgatgtgcagcgcttaagattacga
tcctgcgcgctgatcacggggattgaaacggtactggccgatgatccatcgctcaatgtgcgctactcagagcttggca
gtttaagagcgcaattgagtgagtcgcaggttttacagccgctgcgagtggttttggacagtcgcgcccgcatgcctat
cacggcagcattgtttgcgattgaatcacccattttattagtctcgacagtgagctattcgtcagactttatggtgcaa
ttacccgcccatgtgacttgcctccaattaccagccattgagggtcgaatctccttgtctgcactattaagctatttag
gcaaaagctgtaatcaggtgcttatcgaagcgggcgcgactttagctggcgcctttatcggctcaggattagccgatga
attagtgctttatcaagcgatgaaaatccttggcggccaaggacgtaatctacttgaattacccgattatcaaatgatg
gccgatattccgaccctcaaattggtcgacgagcgtaaagtgggcgcggatacgcgcttcaccttgaggctcgagtcca
atccatctttagcgaataagtgagttaaccatgtttactgggattattgaggccgttggtacactgcgaaagcttgagc
gtaaaggcgatgatattcgcttaacggtcgccagtggcaaactggatttaaccgatgtgcgtttaggcgacagtatcgc
caccaacggtgtatgtttgaccgtggttcaacagttagccgatggctatgtggctgatgtgtcagctgaaaccgtcagc
cttacaggttttgcaaactataaagtgggtactaaggtcaatcttgaaaaggccgtcaccccaactactcgtttaggcg
gccatatggtgagtggtcatgtggatgggattgctactgttgagcagcgtcttgctcgtggtcaagcaatcgaattttg
gttagcggctcccacggagcttgctcgctatattgcccataaaggttctatcacgattgatggcgtgagcctgacagta
aacgaagtcgatggacaccgttttcgattaacgattgtgccccatactgtaggtgaaaccacgttggtggatttaaaag
caggcgataaggtcaatattgaagtggacttaattgcccgttatttagagcgtttaatgcgttttgacagcaaagaaac
ccaaggcggaggggtgaccatggaaatgttagcccgtgctggctttgtgcgttagtacactggcactcagttcacttag
gtatagaatttataacaacagtcacattataaaggtcctacaatggcgctgcacagtatagaagagatcatcgaggata
ttcgtcaaggcaaaatggttattttgatggatgacgaggacagagaaaacgaaggcgatctgattttggcggccgaaat
ggtgacgccagaagcgattaactttatggcgaaatacggccgcgggttaatctgccaaacgatgaccaaggcccgttgc
cagcagttaaatctgcccttgatggtaaccaataacaatgcgcagttctcgaccaactttacggtatcgattgaagctg
ccgaaggcgtaaccacagggatttcggcccacgatcgcgctgtaaccgttaaagcggccgtagcaaaagatgctaaagc
gtcggacttagttcagcctgggcatatcttcccgttaatggcgcaggatggtggcgtattaacccgcgcaggtcacact
gaagcgggctgtgatttagcccgtttagcagggcttgaaccatcgggggtgattgttgaaattttgaacgaagacggca
ctatggcccgtcgcccagatttagagattttctctgaaaaacatggcattaaaatcggcactattgcggccctgattga
ataccgtaataccaaagaaacgaccgttgtgcgtgaagccaaatgcaaactgccaacccgtttcggtgagttcgatatg
gtgacctttagggatactatcgacaatcaattgcactttgccttagtgaaaggcgaggtgaagcctgactgtttagtac
gggtacatttacagaatacctttaacgatctacttcactctgagcgtgatcagcaacgtagctggccgctcgaaaaagc
gatggcgcggatttctgccgaaggtggtgtattagtactgcttggcaatcaggaacattccagtgaaatcctcgccaaa
gtcaaagcctttgaagcggaagatcaaggtcagaccccagtatcggctaaatggcaaggcacctcacgccgtgtaggtg
tgggttcgcaaatcctcgccagtttaggcgtgacaaaaatgcgactcttaagctcgcccaaacgttatcactcactttc
aggctttggccttgaagtgaccgaatgcgtagctgagtaacaggttaagcttgtaataggttgcactaaggtaaaatta
accatttctatcatttgcataaggccgcgggcaattgactgtggtatcatgtcgccacttttcgcccagagctgggtgc
tttagctaaattaggtaagaaaatgaacgtagttcaaggtaatatcgaagcgaagaatgccaaagttgcgattgtaatt
tcgcgttttaacagctttttagtcgaaagcctgcttgaaggtgcacttgacacgctgaaacgttttggccaagtcagtg
atgaaaacatcactgttgtccgtgtacctggtgcggttgagttaccgctggctgcacgtcgtgttgccgcaagtggtaa
gtttgacggtattattgcacttggtgcggtaatccgtggtggtacccctcactttgattttgttgcaggcgaatgtaac
aaaggtctggctcaaattgcattagagttcgatctgcccgttgctttcggtgtattgactacagataccattgaacaag
ctattgagcgttcaggtactaaagcgggtaacaaaggcggcgaagctgctctaagcttgcttgaaatggtcaacgttct
gcaacagcttgaacaacagttgtaa