##gff-version 3
##sequence-region BBa_K1172304 1 3635
BBa_K1172304	.	CDS	39	3635	.	+	0	ID=BBa_K1172303;Name=BBa_K1172303
BBa_K1172304	.	stop_codon	3633	3635	.	+	0	ID=annotation2370724;Name=taa;Parent=BBa_K1172303
BBa_K1172304	.	start_codon	1192	1194	.	+	0	ID=annotation2370718;Name=atg;Parent=BBa_K1172303
BBa_K1172304	.	start_codon	39	41	.	+	0	ID=annotation2370717;Name=atg;Parent=BBa_K1172303
BBa_K1172304	.	stop_codon	1846	1848	.	+	0	ID=annotation2370722;Name=tag;Parent=BBa_K1172303
BBa_K1172304	.	start_codon	3159	3161	.	+	0	ID=annotation2370720;Name=atg;Parent=BBa_K1172303
BBa_K1172304	.	point_mutation	272	277	.	+	0	ID=annotation2370714;Name=Pst1 C->T;Parent=BBa_K1172303
BBa_K1172304	.	CDS	1192	1848	.	+	0	ID=annotation2370311;Name=SO_3468;Parent=BBa_K1172303
BBa_K1172304	.	stop_codon	3016	3018	.	+	0	ID=annotation2370723;Name=taa;Parent=BBa_K1172303
BBa_K1172304	.	point_mutation	1682	1687	.	+	0	ID=annotation2370715;Name=Pst1 C->T;Parent=BBa_K1172303
BBa_K1172304	.	CDS	39	1184	.	+	0	ID=annotation2370310;Name=ribD;Parent=BBa_K1172303
BBa_K1172304	.	stop_codon	1182	1184	.	+	0	ID=annotation2370721;Name=tga;Parent=BBa_K1172303
BBa_K1172304	.	CDS	3159	3635	.	+	0	ID=annotation2370313;Name=ribE;Parent=BBa_K1172303
BBa_K1172304	.	CDS	1915	3018	.	+	0	ID=annotation2370312;Name=ribBA;Parent=BBa_K1172303
BBa_K1172304	.	point_mutation	1878	1883	.	+	0	ID=annotation2370716;Name=EcoR1 C->T;Parent=BBa_K1172303
BBa_K1172304	.	start_codon	1915	1917	.	+	0	ID=annotation2370719;Name=atg;Parent=BBa_K1172303
BBa_K1172304	.	promoter	1	32	.	+	0	ID=BBa_K525998;Name=BBa_K525998
BBa_K1172304	.	promoter	1	20	.	+	0	ID=annotation2129432;Name=T7 promoter;Parent=BBa_K525998
BBa_K1172304	.	ribosome_entry_site	24	27	.	+	0	ID=annotation2129433;Name=RBS;Parent=BBa_K525998
BBa_K1172304	.	engineered_region	21	32	.	+	0	ID=annotation2127788;Name=B0034;Parent=BBa_K525998
>BBa_K1172304
taatacgactcactatagggaaagaggagaaatactagatgaattggtcagtactcgataaccaaatgatgagccgagc
tattcaattggctcgcaaaggattttataccactcgccccaatcccagtgtgggctgcgttatcgttaacgataaccag
attgtcggtgaaggttatcatcaaaaagccggagagccccacgctgaagtgcatgcgctgcgcatggctggcgagcttg
cccgtggcgcgactgcttatgtcaccttagaaccctgtagccattatggtcgtacgccgccctgcgccttggcgctgat
caatattggtgtaaagcgggtggtggtggcggttgaagatcctaatccgcaggtcggcgggcgcggtattcaaatgctg
cgcgatgcgggtattcaagtggatgtgggcttgcatcgtgatgaggcctatgccttaaatcttggttttatgaagcgca
tggagtcgggtctaccttgggtgacggtaaagcttgccgcgagtcttgatggtaaaactgcgttatcaaacggggtgtc
taaatggatcactggccctgaagctcgccgcgatgtgcagcgcttaagattacgatcctgcgcgctgatcacggggatt
gaaacggtactggccgatgatccatcgctcaatgtgcgctactcagagcttggcagtttaagagcgcaattgagtgagt
cgcaggttttacagccgctgcgagtggttttggacagtcgcgcccgcatgcctatcacggcagcattgtttgcgattga
atcacccattttattagtctcgacagtgagctattcgtcagactttatggtgcaattacccgcccatgtgacttgcctc
caattaccagccattgagggtcgaatctccttgtctgcactattaagctatttaggcaaaagctgtaatcaggtgctta
tcgaagcgggcgcgactttagctggcgcctttatcggctcaggattagccgatgaattagtgctttatcaagcgatgaa
aatccttggcggccaaggacgtaatctacttgaattacccgattatcaaatgatggccgatattccgaccctcaaattg
gtcgacgagcgtaaagtgggcgcggatacgcgcttcaccttgaggctcgagtccaatccatctttagcgaataagtgag
ttaaccatgtttactgggattattgaggccgttggtacactgcgaaagcttgagcgtaaaggcgatgatattcgcttaa
cggtcgccagtggcaaactggatttaaccgatgtgcgtttaggcgacagtatcgccaccaacggtgtatgtttgaccgt
ggttcaacagttagccgatggctatgtggctgatgtgtcagctgaaaccgtcagccttacaggttttgcaaactataaa
gtgggtactaaggtcaatcttgaaaaggccgtcaccccaactactcgtttaggcggccatatggtgagtggtcatgtgg
atgggattgctactgttgagcagcgtcttgctcgtggtcaagcaatcgaattttggttagcggctcccacggagcttgc
tcgctatattgcccataaaggttctatcacgattgatggcgtgagcctgacagtaaacgaagtcgatggacaccgtttt
cgattaacgattgtgccccatactgtaggtgaaaccacgttggtggatttaaaagcaggcgataaggtcaatattgaag
tggacttaattgcccgttatttagagcgtttaatgcgttttgacagcaaagaaacccaaggcggaggggtgaccatgga
aatgttagcccgtgctggctttgtgcgttagtacactggcactcagttcacttaggtatagaatttataacaacagtca
cattataaaggtcctacaatggcgctgcacagtatagaagagatcatcgaggatattcgtcaaggcaaaatggttattt
tgatggatgacgaggacagagaaaacgaaggcgatctgattttggcggccgaaatggtgacgccagaagcgattaactt
tatggcgaaatacggccgcgggttaatctgccaaacgatgaccaaggcccgttgccagcagttaaatctgcccttgatg
gtaaccaataacaatgcgcagttctcgaccaactttacggtatcgattgaagctgccgaaggcgtaaccacagggattt
cggcccacgatcgcgctgtaaccgttaaagcggccgtagcaaaagatgctaaagcgtcggacttagttcagcctgggca
tatcttcccgttaatggcgcaggatggtggcgtattaacccgcgcaggtcacactgaagcgggctgtgatttagcccgt
ttagcagggcttgaaccatcgggggtgattgttgaaattttgaacgaagacggcactatggcccgtcgcccagatttag
agattttctctgaaaaacatggcattaaaatcggcactattgcggccctgattgaataccgtaataccaaagaaacgac
cgttgtgcgtgaagccaaatgcaaactgccaacccgtttcggtgagttcgatatggtgacctttagggatactatcgac
aatcaattgcactttgccttagtgaaaggcgaggtgaagcctgactgtttagtacgggtacatttacagaataccttta
acgatctacttcactctgagcgtgatcagcaacgtagctggccgctcgaaaaagcgatggcgcggatttctgccgaagg
tggtgtattagtactgcttggcaatcaggaacattccagtgaaatcctcgccaaagtcaaagcctttgaagcggaagat
caaggtcagaccccagtatcggctaaatggcaaggcacctcacgccgtgtaggtgtgggttcgcaaatcctcgccagtt
taggcgtgacaaaaatgcgactcttaagctcgcccaaacgttatcactcactttcaggctttggccttgaagtgaccga
atgcgtagctgagtaacaggttaagcttgtaataggttgcactaaggtaaaattaaccatttctatcatttgcataagg
ccgcgggcaattgactgtggtatcatgtcgccacttttcgcccagagctgggtgctttagctaaattaggtaagaaaat
gaacgtagttcaaggtaatatcgaagcgaagaatgccaaagttgcgattgtaatttcgcgttttaacagctttttagtc
gaaagcctgcttgaaggtgcacttgacacgctgaaacgttttggccaagtcagtgatgaaaacatcactgttgtccgtg
tacctggtgcggttgagttaccgctggctgcacgtcgtgttgccgcaagtggtaagtttgacggtattattgcacttgg
tgcggtaatccgtggtggtacccctcactttgattttgttgcaggcgaatgtaacaaaggtctggctcaaattgcatta
gagttcgatctgcccgttgctttcggtgtattgactacagataccattgaacaagctattgagcgttcaggtactaaag
cgggtaacaaaggcggcgaagctgctctaagcttgcttgaaatggtcaacgttctgcaacagcttgaacaacagttgtaa
a