##gff-version 3
##sequence-region BBa_K1132017 1 5689
BBa_K1132017	.	CDS	2474	5689	.	+	0	ID=BBa_K1132016;Name=BBa_K1132016
BBa_K1132017	.	engineered_region	2590	3363	.	+	0	ID=BBa_K081014;Name=BBa_K081014;Parent=BBa_K1132016
BBa_K1132017	.	CDS	2611	3316	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_K081014
BBa_K1132017	.	CDS	2611	3285	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K1132017	.	engineered_tag	3292	3316	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K1132017	.	ribosome_entry_site	2590	2604	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K081014
BBa_K1132017	.	ribosome_entry_site	2590	2604	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K1132017	.	ribosome_entry_site	2597	2601	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K1132017	.	engineered_region	2590	2604	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K1132017	.	terminator	3325	3363	.	+	0	ID=BBa_B1006;Name=BBa_B1006;Parent=BBa_K081014
BBa_K1132017	.	stem_loop	3334	3355	.	+	0	ID=annotation1898429;Name=modified thr terminator;Parent=BBa_B1006
BBa_K1132017	.	polyA_site	3325	3333	.	+	0	ID=annotation1898431;Name=PolyA;Parent=BBa_B1006
BBa_K1132017	.	polyA_site	3356	3363	.	+	0	ID=annotation1898430;Name=PolyA;Parent=BBa_B1006
BBa_K1132017	.	engineered_region	3325	3363	.	+	0	ID=annotation1898428;Name=B1006;Parent=BBa_B1006
BBa_K1132017	.	promoter	3372	3425	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_K1132016
BBa_K1132017	.	promoter	3391	3396	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K1132017	.	engineered_region	3372	3425	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K1132017	.	promoter	3414	3419	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K1132017	.	non_covalent_binding_site	3372	3390	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K1132017	.	non_covalent_binding_site	3397	3415	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K1132017	.	promoter	2474	2581	.	+	0	ID=BBa_R0082;Name=BBa_R0082;Parent=BBa_K1132016
BBa_K1132017	.	non_covalent_binding_site	2486	2503	.	+	0	ID=annotation301154;Name=C1 OmpR;Parent=BBa_R0082
BBa_K1132017	.	promoter	2548	2553	.	+	0	ID=annotation301166;Name=-35;Parent=BBa_R0082
BBa_K1132017	.	non_covalent_binding_site	2527	2544	.	+	0	ID=annotation301156;Name=C3 OmpR;Parent=BBa_R0082
BBa_K1132017	.	promoter	2571	2576	.	+	0	ID=annotation301167;Name=-10;Parent=BBa_R0082
BBa_K1132017	.	non_covalent_binding_site	2507	2524	.	+	0	ID=annotation301155;Name=C2 OmpR;Parent=BBa_R0082
BBa_K1132017	.	engineered_region	3434	5689	.	+	0	ID=BBa_K592018;Name=BBa_K592018;Parent=BBa_K1132016
BBa_K1132017	.	CDS	3452	5689	.	+	0	ID=BBa_K592000;Name=BBa_K592000;Parent=BBa_K592018
BBa_K1132017	.	CDS	3452	5689	.	+	0	ID=annotation2132074;Name=cph8;Parent=BBa_K592000
BBa_K1132017	.	ribosome_entry_site	3434	3445	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K592018
BBa_K1132017	.	conserved_region	3438	3441	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1132017	.	CDS	1	2465	.	+	0	ID=BBa_K1132013;Name=BBa_K1132013
BBa_K1132017	.	CDS	63	2465	.	+	0	ID=BBa_K1132012;Name=BBa_K1132012;Parent=BBa_K1132013
BBa_K1132017	.	CDS	63	1617	.	+	0	ID=BBa_K1132011;Name=BBa_K1132011;Parent=BBa_K1132012
BBa_K1132017	.	engineered_region	822	1617	.	+	0	ID=BBa_K081017;Name=BBa_K081017;Parent=BBa_K1132011
BBa_K1132017	.	ribosome_entry_site	822	836	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K081017
BBa_K1132017	.	ribosome_entry_site	822	836	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K1132017	.	ribosome_entry_site	829	833	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K1132017	.	engineered_region	822	836	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K1132017	.	CDS	843	1617	.	+	0	ID=BBa_I15009;Name=BBa_I15009;Parent=BBa_K081017
BBa_K1132017	.	engineered_tag	1593	1617	.	+	0	ID=annotation2214032;Name=Help:Barcodes;Parent=BBa_I15009
BBa_K1132017	.	CDS	843	1592	.	+	0	ID=annotation1891612;Name=PcyA;Parent=BBa_I15009
BBa_K1132017	.	CDS	63	813	.	+	0	ID=BBa_I15008;Name=BBa_I15008;Parent=BBa_K1132011
BBa_K1132017	.	engineered_tag	789	813	.	+	0	ID=annotation2214026;Name=Help:Barcodes;Parent=BBa_I15008
BBa_K1132017	.	engineered_region	1626	2465	.	+	0	ID=BBa_P0440;Name=BBa_P0440;Parent=BBa_K1132012
BBa_K1132017	.	terminator	2337	2416	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0440
BBa_K1132017	.	engineered_region	2337	2416	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1132017	.	stem_loop	2348	2391	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1132017	.	CDS	1644	2303	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_P0440
BBa_K1132017	.	CDS	1647	2263	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K1132017	.	tag	2264	2297	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K1132017	.	engineered_tag	2304	2328	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K1132017	.	ribosome_entry_site	1626	1637	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_P0440
BBa_K1132017	.	conserved_region	1630	1633	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1132017	.	terminator	2425	2465	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0440
BBa_K1132017	.	engineered_region	2425	2465	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1132017	.	polyA_site	2452	2465	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1132017	.	stem_loop	2432	2451	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1132017	.	stop_codon	2458	2458	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1132017	.	engineered_region	1	56	.	+	0	ID=BBa_K608003;Name=BBa_K608003;Parent=BBa_K1132013
BBa_K1132017	.	promoter	1	35	.	+	0	ID=BBa_J23104;Name=BBa_J23104;Parent=BBa_K608003
BBa_K1132017	.	ribosome_entry_site	44	56	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K608003
BBa_K1132017	.	engineered_region	44	56	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K1132017	.	ribosome_entry_site	44	56	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K1132017	.	ribosome_entry_site	50	53	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
>BBa_K1132017
ttgacagctagctcagtcctaggtattgtgctagctactagagtcacacaggaaagtactagatgagtgtcaacttagc
ttcccagttgcgggaagggacgaaaaaatcccactccatggcggagaacgtcggctttgtcaaatgcttcctcaagggc
gttgtcgagaaaaattcctaccgtaagctggttggcaatctctactttgtctacagtgccatggaagaggaaatggcaa
aatttaaggaccatcccatcctcagccacatttacttccccgaactcaaccgcaaacaaagcctagagcaagacctgca
attctattacggctccaactggcggcaagaagtgaaaatttctgccgctggccaagcctatgtggaccgagtccggcaa
gtggccgctacggcccctgaattgttggtggcccattcctacacccgttacctgggggatctttccggcggtcaaattc
tcaagaaaattgcccaaaatgccatgaatctccacgatggtggcacagctttctatgaatttgccgacattgatgacga
aaaggcttttaaaaatacctaccgtcaagctatgaatgatctgcccattgaccaagccaccgccgaacggattgtggat
gaagccaatgacgcctttgccatgaacatgaaaatgttcaacgaacttgaaggcaacctgatcaaggcgatcggcatta
tggtgttcaacagcctcacccgtcgccgcagtcaaggcagcaccgaagttggcctcgccacctccgaaggctaataacg
ctgatagtgctagtgtagatcgctactagagattaaagaggagaaatactagatggccgtcactgatttaagtttgacc
aattcttccctgatgcctacgttgaacccgatgattcaacagttggccctggcgatcgccgctagttggcaaagtttac
ccctcaagccctatcaattgccggaggatttgggctacgtagaaggccgcctggaaggggaaaagttagtgattgaaaa
tcggtgctaccaaacgccccagtttcgcaaaatgcatttggagttggccaaggtgggcaaagggttggatattctccac
tgtgtaatgtttcctgagcctttatacggtctacctttgtttggctgtgacattgtggccggccccggtggagtaagtg
cggctattgcggatctatcccccacccaaagcgatcgccaattgcccgcagcgtaccaaaaatcattggcagagctagg
ccagccagaatttgagcaacaacgggaattgcccccctggggagaaatattttctgaatattgtttattcatccgtccc
agcaatgtcactgaagaagaaagatttgtacaaagggtagtggactttttgcaaattcattgtcaccaatccatcgttg
ccgaacccttgtctgaagctcaaactttggagcaccgtcaggggcaaattcattactgccaacaacaacagaaaaatga
taaaacccgtcgggtactggaaaaagcttttggggaagcttgggcggaacggtatatgagccaagtcttatttgatgtt
atccaataataactctgatagtgctagtgtagatctctactagagaaagaggagaaatactagatgtccagattagata
aaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgccca
gaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgag
atgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaa
gttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatga
aactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtg
gggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactg
atagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggcct
tgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgcttta
gtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaa
agactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtggg
cctttctgcgtttatatactagagtcccttgcatttacattttgaaacatctatagcgataaatgaaacatcttaaaag
ttttagtatcatattcgtgttggattattctgcatttttggggagaatggacttactagagattaaagaggagaaatac
tagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacg
agttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtgg
tccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgac
atcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttg
ttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtc
cgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctg
aaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggcta
aaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccat
cgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgcta
ctagagaaaaaaaaaccccgcccctgacagggcggggtttttttttactagagtccctatcagtgatagagattgacat
ccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatggccaccaccgtacaactcagcg
accaatccctccgtcagctagaaaccctcgccatccacaccgcccacctgattcagccccacggtttagtggtggtcct
gcaagaaccagacctcaccatcagccaaattagcgccaactgcaccggcattttagggcgatcgccagaggatttgttg
ggcagaaccctaggggaagtgtttgatagctttcagattgatcccatccagagtcgcctaacggccggacaaatcagca
gcctcaaccccagtaaactttgggcgcgggtcatgggggacgactttgtcatttttgacggggtttttcatcgcaacag
tgacggtttattggtatgtgaactcgagccagcctacacttccgataatctgcccttcctcggtttttatcacatggcc
aacgctgccctgaatcggttgcgccaacaagctaatctacgggatttctacgatgttattgtcgaagaagtccgccgta
tgactggctttgaccgggtgatgctataccgctttgatgaaaataaccacggtgatgtcattgccgaagataaacggga
tgatatggaaccctatttgggcctgcactatcccgaatcggatattccccaacccgcccgtcggctatttatccacaac
cccattcgagtaattcccgatgtttatggtgtggcggtgcccctgaccccagcggttaaccccagcaccaaccgagcgg
tggatttaacagaatccattctgcgcagtgcgtaccattgccacttgacctatctgaaaaatatgggggtaggagcgtc
tttaaccatttccctaattaaggacggccatctctgggggctcattgcctgccaccatcaaacccccaaagtaattccc
tttgaactgcgtaaagcctgcgaattttttggtcgggtggtgtttagcaacatttccgcccaggaagatacggaaacct
tcgattaccgggtgcagctggcggagcatgaagcggttttattggacaaaatgaccacggcggcggattttgtcgaagg
attaactaatcatcccgatcgcctgttgggattaacgggctcccagggggcggccatttgctttggggaaaaattgatt
ttagtaggggaaaccccggacgagaaagcagtgcaatatttactgcaatggttggagaatcgggaagtgcaagacgttt
tcttcacctcttccctctcacaaatttatcctgatgcagtgaattttaaatccgtggccagtggcttattggccattcc
cattgcccgtcacaactttttgctctggtttcgccctgaagtgttgcaaacggttaattggggcggtgacccaaatcat
gcttacgaagctacccaggaagacggtaaaatcgagctccatccccgccaatcctttgacctctggaaagaaattgtcc
gactccaatctttgccctggcaatcggtggaaatccaaagtgccctggccctgaaaaaggcgatcgtcaacctcatttt
gcgccaggcagaagaattgcatatggcggctggtgttaagcaactggcggatgaccgcacgctgctgatggcgggggta
agtcacgacttgcgcacgccgctgacgcgtattcgcctggcgactgagatgatgagcgagcaggatggctatctggcag
aatcgatcaataaagatatcgaagagtgcaacgccatcattgagcagtttatcgactacctgcgcaccgggcaggagat
gccgatggaaatggcggatcttaatgcagtactcggtgaggtgattgctgccgaaagtggctatgagcgggaaattgaa
accgcgctttaccccggcagcattgaagtgaaaatgcacccgctgtcgatcaaacgcgcggtggcgaatatggtggtca
acgccgcccgttatggcaatggctgggtcaaagtcagcagcggaacggagccgaatcgcgcctggttccaggtggaaga
tgacggtccgggaattgcgccggaacaacgtaagcacctgttccagccgtttgtccgcggcgacagtgcgcgcaccatt
agcggcacgggattagggctggcaattgtgcagcgtatcgtggataaccataacgggatgctggagcttggcaccagcg
agcggggcgggctttccattcgcgcctggctgccagtgccggtaacgcgggcgcagggcatgacaaaagaagggtaataa
a