LOCUS       BBa_K1132016            3216 bp    DNA     linear       11-Dec-2025
DEFINITION  Promoter (OmpR, positive) followed by RFP protein generator and 
            Cph8 (Cph1/EnvZ fusion) under TetR r
ACCESSION   BBa_K1132016
VERSION     BBa_K1132016.1  
FEATURES             Location/Qualifiers
     misc_feature    13..30
                     /label=C1 OmpR
     misc_feature    34..51
                     /label=C2 OmpR
     misc_feature    54..71
                     /label=C3 OmpR
     promoter        75..80
                     /label=-35
     promoter        98..103
                     /label=-10
     promoter        1..108
                     /label=BBa_R0082
     RBS             117..131
                     /label=RBS-1\Strong
     RBS             124..128
                     /label=RBS
     misc_feature    117..131
                     /label=BBa_B0030
     RBS             117..131
                     /label=BBa_B0030
     CDS             138..812
                     /label=mrfp1
     misc_feature    819..843
                     /label=Help:Barcodes
     CDS             138..843
                     /label=BBa_E1010
     misc_feature    852..890
                     /label=B1006
     stem_loop       861..882
                     /label=modified thr terminator
     polyA_site      883..890
                     /label=PolyA
     polyA_site      852..860
                     /label=PolyA
     terminator      852..890
                     /label=BBa_B1006
     misc_feature    117..890
                     /label=BBa_K081014
     misc_feature    899..917
                     /label=TetR 1
     misc_feature    899..952
                     /label=BBa_R0040
     promoter        918..923
                     /label=-35
     misc_feature    924..942
                     /label=TetR 2
     promoter        941..946
                     /label=-10
     promoter        899..952
                     /label=BBa_R0040
     misc_feature    965..968
                     /label=conserved
     RBS             961..972
                     /label=BBa_B0034
     CDS             979..3216
                     /label=cph8
     CDS             979..3216
                     /label=BBa_K592000
     misc_feature    961..3216
                     /label=BBa_K592018
ORIGIN
        1 tcccttgcat ttacattttg aaacatctat agcgataaat gaaacatctt aaaagtttta
       61 gtatcatatt cgtgttggat tattctgcat ttttggggag aatggactta ctagagatta
      121 aagaggagaa atactagatg gcttcctccg aagacgttat caaagagttc atgcgtttca
      181 aagttcgtat ggaaggttcc gttaacggtc acgagttcga aatcgaaggt gaaggtgaag
      241 gtcgtccgta cgaaggtacc cagaccgcta aactgaaagt taccaaaggt ggtccgctgc
      301 cgttcgcttg ggacatcctg tccccgcagt tccagtacgg ttccaaagct tacgttaaac
      361 acccggctga catcccggac tacctgaaac tgtccttccc ggaaggtttc aaatgggaac
      421 gtgttatgaa cttcgaagac ggtggtgttg ttaccgttac ccaggactcc tccctgcaag
      481 acggtgagtt catctacaaa gttaaactgc gtggtaccaa cttcccgtcc gacggtccgg
      541 ttatgcagaa aaaaaccatg ggttgggaag cttccaccga acgtatgtac ccggaagacg
      601 gtgctctgaa aggtgaaatc aaaatgcgtc tgaaactgaa agacggtggt cactacgacg
      661 ctgaagttaa aaccacctac atggctaaaa aaccggttca gctgccgggt gcttacaaaa
      721 ccgacatcaa actggacatc acctcccaca acgaagacta caccatcgtt gaacagtacg
      781 aacgtgctga aggtcgtcac tccaccggtg cttaataacg ctgatagtgc tagtgtagat
      841 cgctactaga gaaaaaaaaa ccccgcccct gacagggcgg ggtttttttt tactagagtc
      901 cctatcagtg atagagattg acatccctat cagtgataga gatactgagc actactagag
      961 aaagaggaga aatactagat ggccaccacc gtacaactca gcgaccaatc cctccgtcag
     1021 ctagaaaccc tcgccatcca caccgcccac ctgattcagc cccacggttt agtggtggtc
     1081 ctgcaagaac cagacctcac catcagccaa attagcgcca actgcaccgg cattttaggg
     1141 cgatcgccag aggatttgtt gggcagaacc ctaggggaag tgtttgatag ctttcagatt
     1201 gatcccatcc agagtcgcct aacggccgga caaatcagca gcctcaaccc cagtaaactt
     1261 tgggcgcggg tcatggggga cgactttgtc atttttgacg gggtttttca tcgcaacagt
     1321 gacggtttat tggtatgtga actcgagcca gcctacactt ccgataatct gcccttcctc
     1381 ggtttttatc acatggccaa cgctgccctg aatcggttgc gccaacaagc taatctacgg
     1441 gatttctacg atgttattgt cgaagaagtc cgccgtatga ctggctttga ccgggtgatg
     1501 ctataccgct ttgatgaaaa taaccacggt gatgtcattg ccgaagataa acgggatgat
     1561 atggaaccct atttgggcct gcactatccc gaatcggata ttccccaacc cgcccgtcgg
     1621 ctatttatcc acaaccccat tcgagtaatt cccgatgttt atggtgtggc ggtgcccctg
     1681 accccagcgg ttaaccccag caccaaccga gcggtggatt taacagaatc cattctgcgc
     1741 agtgcgtacc attgccactt gacctatctg aaaaatatgg gggtaggagc gtctttaacc
     1801 atttccctaa ttaaggacgg ccatctctgg gggctcattg cctgccacca tcaaaccccc
     1861 aaagtaattc cctttgaact gcgtaaagcc tgcgaatttt ttggtcgggt ggtgtttagc
     1921 aacatttccg cccaggaaga tacggaaacc ttcgattacc gggtgcagct ggcggagcat
     1981 gaagcggttt tattggacaa aatgaccacg gcggcggatt ttgtcgaagg attaactaat
     2041 catcccgatc gcctgttggg attaacgggc tcccaggggg cggccatttg ctttggggaa
     2101 aaattgattt tagtagggga aaccccggac gagaaagcag tgcaatattt actgcaatgg
     2161 ttggagaatc gggaagtgca agacgttttc ttcacctctt ccctctcaca aatttatcct
     2221 gatgcagtga attttaaatc cgtggccagt ggcttattgg ccattcccat tgcccgtcac
     2281 aactttttgc tctggtttcg ccctgaagtg ttgcaaacgg ttaattgggg cggtgaccca
     2341 aatcatgctt acgaagctac ccaggaagac ggtaaaatcg agctccatcc ccgccaatcc
     2401 tttgacctct ggaaagaaat tgtccgactc caatctttgc cctggcaatc ggtggaaatc
     2461 caaagtgccc tggccctgaa aaaggcgatc gtcaacctca ttttgcgcca ggcagaagaa
     2521 ttgcatatgg cggctggtgt taagcaactg gcggatgacc gcacgctgct gatggcgggg
     2581 gtaagtcacg acttgcgcac gccgctgacg cgtattcgcc tggcgactga gatgatgagc
     2641 gagcaggatg gctatctggc agaatcgatc aataaagata tcgaagagtg caacgccatc
     2701 attgagcagt ttatcgacta cctgcgcacc gggcaggaga tgccgatgga aatggcggat
     2761 cttaatgcag tactcggtga ggtgattgct gccgaaagtg gctatgagcg ggaaattgaa
     2821 accgcgcttt accccggcag cattgaagtg aaaatgcacc cgctgtcgat caaacgcgcg
     2881 gtggcgaata tggtggtcaa cgccgcccgt tatggcaatg gctgggtcaa agtcagcagc
     2941 ggaacggagc cgaatcgcgc ctggttccag gtggaagatg acggtccggg aattgcgccg
     3001 gaacaacgta agcacctgtt ccagccgttt gtccgcggcg acagtgcgcg caccattagc
     3061 ggcacgggat tagggctggc aattgtgcag cgtatcgtgg ataaccataa cgggatgctg
     3121 gagcttggca ccagcgagcg gggcgggctt tccattcgcg cctggctgcc agtgccggta
     3181 acgcgggcgc agggcatgac aaaagaaggg taataa
//