##gff-version 3
##sequence-region BBa_K1087023 1 1869
BBa_K1087023	.	engineered_region	407	1869	.	+	0	ID=BBa_K1087022;Name=BBa_K1087022
BBa_K1087023	.	engineered_region	909	1869	.	+	0	ID=BBa_K1087018;Name=BBa_K1087018;Parent=BBa_K1087022
BBa_K1087023	.	engineered_region	1009	1869	.	+	0	ID=BBa_I13507;Name=BBa_I13507;Parent=BBa_K1087018
BBa_K1087023	.	CDS	1027	1707	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_I13507
BBa_K1087023	.	engineered_tag	1708	1732	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K1087023	.	CDS	1027	1701	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K1087023	.	terminator	1741	1820	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13507
BBa_K1087023	.	engineered_region	1741	1820	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1087023	.	stem_loop	1752	1795	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1087023	.	ribosome_entry_site	1009	1020	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13507
BBa_K1087023	.	conserved_region	1013	1016	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1087023	.	terminator	1829	1869	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13507
BBa_K1087023	.	stop_codon	1862	1862	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1087023	.	polyA_site	1856	1869	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1087023	.	stem_loop	1836	1855	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1087023	.	engineered_region	1829	1869	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1087023	.	promoter	909	1000	.	+	0	ID=BBa_I746361;Name=BBa_I746361;Parent=BBa_K1087018
BBa_K1087023	.	promoter	909	1000	.	+	0	ID=annotation1943784;Name=PO;Parent=BBa_I746361
BBa_K1087023	.	promoter	407	900	.	+	0	ID=BBa_K1087007;Name=BBa_K1087007;Parent=BBa_K1087022
BBa_K1087023	.	engineered_region	518	900	.	+	0	ID=BBa_K1087006;Name=BBa_K1087006;Parent=BBa_K1087007
BBa_K1087023	.	promoter	407	511	.	+	0	ID=BBa_K1087004;Name=BBa_K1087004;Parent=BBa_K1087007
BBa_K1087023	.	mature_transcript_region	469	511	.	+	0	ID=BBa_J23032;Name=BBa_J23032;Parent=BBa_K1087004
BBa_K1087023	.	promoter	407	460	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_K1087004
BBa_K1087023	.	engineered_region	407	460	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K1087023	.	promoter	426	431	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K1087023	.	promoter	449	454	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K1087023	.	non_covalent_binding_site	407	425	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K1087023	.	non_covalent_binding_site	432	450	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K1087023	.	engineered_region	1	398	.	+	0	ID=BBa_K145215;Name=BBa_K145215
BBa_K1087023	.	terminator	358	398	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K145215
BBa_K1087023	.	stop_codon	391	391	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1087023	.	polyA_site	385	398	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1087023	.	stem_loop	365	384	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1087023	.	engineered_region	358	398	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1087023	.	mature_transcript_region	165	261	.	+	0	ID=BBa_J23009;Name=BBa_J23009;Parent=BBa_K145215
BBa_K1087023	.	mature_transcript_region	63	156	.	+	0	ID=BBa_J23008;Name=BBa_J23008;Parent=BBa_K145215
BBa_K1087023	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_K145215
BBa_K1087023	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K1087023	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K1087023	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K1087023	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K1087023	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K1087023	.	terminator	270	349	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K145215
BBa_K1087023	.	engineered_region	270	349	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1087023	.	stem_loop	281	324	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
>BBa_K1087023
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagacccaaaagcaagaggt
gattctagttggtggttaatgaaaattaacttacttactagaaatatctctaaaaagccagattattaatccggcttta
ctagagcgggcgggcgggcgggcgggatccataaaaaaaaaacccaaaagcaagaggtgattctagttaaaaaaaaaaa
gcttcccgcccgcccgcccgcccgtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggccttt
cgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgttt
atatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaactag
aatcacctcttgcttttgggtaagacagaagaggagatactagatgatgcgctgccctttctgtcgtcattcagcgcat
acccgcaccagccggtatgtgagtgacaatgtcaaagaaagttatctccagtgccagaatatttactgttcggcgacat
ttaaaacgcatgagtcaatttgtgccgtgattcgttctccggtcacggaggaaaaaccagcaccggcaagcacagcacc
ggctgttgtccgaaaagttaaaggctgttacagctcaccattcaaccattaatactagagccaggcatcaaataaaacg
aaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggc
tcaccttcgggtgggcctttctgcgtttatatactagagcgcgccccgcgattcgctaaggtgctgttgtgtcagtgat
aagccatccgggactgatggcggaggatgcgcatcgtcgggaaactgatgcctactagagaaagaggagaaatactaga
tggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagtt
cgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccg
ctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcc
cggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttac
cgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgac
ggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaag
gtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaa
accggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgtt
gaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactag
agccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgct
ctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata