##gff-version 3
##sequence-region BBa_K1075024 1 1657
BBa_K1075024	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K1075024	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1075024	.	engineered_region	19	732	.	+	0	ID=BBa_J06504;Name=BBa_J06504
BBa_K1075024	.	CDS	19	729	.	+	0	ID=annotation1585829;Name=mCherry;Parent=BBa_J06504
BBa_K1075024	.	sequence_alteration	370	370	.	+	0	ID=annotation1585833;Name=C->T (removing PstI site);Parent=BBa_J06504
BBa_K1075024	.	engineered_region	739	1657	.	+	0	ID=BBa_K1075023;Name=BBa_K1075023
BBa_K1075024	.	engineered_region	739	1461	.	+	0	ID=BBa_J06505;Name=BBa_J06505;Parent=BBa_K1075023
BBa_K1075024	.	CDS	739	1446	.	+	0	ID=annotation1585879;Name=mCherry;Parent=BBa_J06505
BBa_K1075024	.	sequence_alteration	1090	1090	.	+	0	ID=annotation1585880;Name=C->T (removing PstI site);Parent=BBa_J06505
BBa_K1075024	.	tag	1447	1455	.	+	0	ID=annotation1585887;Name=LVA;Parent=BBa_J06505
BBa_K1075024	.	tag	1470	1520	.	+	0	ID=BBa_K1075020;Name=BBa_K1075020;Parent=BBa_K1075023
BBa_K1075024	.	terminator	1529	1657	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1075023
BBa_K1075024	.	terminator	1529	1608	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1075024	.	stem_loop	1540	1583	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1075024	.	engineered_region	1529	1608	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1075024	.	terminator	1617	1657	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1075024	.	stop_codon	1650	1650	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1075024	.	engineered_region	1617	1657	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1075024	.	stem_loop	1624	1643	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1075024	.	polyA_site	1644	1657	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
>BBa_K1075024
aaagaggagaaatactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaagg
tgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcaccca
gaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggc
tccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagc
gcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaa
ggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctcc
gagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacg
acgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttgga
catcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatg
gacgagctgtacaagtaataatactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgc
gcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacga
gggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttc
atgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttca
agtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagtt
catctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggag
gcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcg
gccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacat
caagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccacc
ggcggcatggacgagctgtacaagttagtagcttaataatactagaggcagctaacgatgaaaactacagcgaaaacta
tgctgacgctagctaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttt
tatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata