Sir3

BBa_K106695 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K106695

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Andrew Horwitz

Date created: 2008-10-28 12:00:00

Date modified: 2015-05-08 01:09:00

Sir3 under a strong constitutive promoter

Details

• Types: DnaRegion
• Roles: engineered_region; Generator
• Sequences: BBa_K106695_sequence (Version 1)

Description

Adh1P!-Sir3-Adh1t

Sir3 is expressed from the high constitutive promoter Adh1. This was cloned by AarI, using the A! fusion (no amino acids added to the N-terminus). This is important, as Sir3 does not tolerate N-terminal extensions.

Notes

Sir3 was cloned as an A!D ended part.

Source

Sir3 from genomic DNA.

Components

Access	Instance	Definition
public	BBa_K106694	BBa_K106694
public	BBa_K106692	BBa_K106692
public	BBa_K106017	BBa_K106017
public	BBa_K106018	BBa_K106018

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K106694	1,1511	BBa_K106694
BBa_K106692	1512,4448	BBa_K106692
BBa_K106017	4449,4477	BBa_K106017
BBa_K106018	4478,4657	BBa_K106018

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K106695/1

Member of these Collections

• iGEM Parts Registry