##gff-version 3
##sequence-region BBa_K1060012 1 3222
BBa_K1060012	.	terminator	3094	3222	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1060012	.	terminator	3182	3222	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1060012	.	stop_codon	3215	3215	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1060012	.	polyA_site	3209	3222	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1060012	.	stem_loop	3189	3208	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1060012	.	engineered_region	3182	3222	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1060012	.	terminator	3094	3173	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1060012	.	engineered_region	3094	3173	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1060012	.	stem_loop	3105	3148	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1060012	.	promoter	1269	1322	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K1060012	.	non_covalent_binding_site	1294	1312	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K1060012	.	promoter	1311	1316	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K1060012	.	engineered_region	1269	1322	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K1060012	.	non_covalent_binding_site	1269	1287	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K1060012	.	promoter	1288	1293	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K1060012	.	ribosome_entry_site	1331	1343	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_K1060012	.	ribosome_entry_site	1331	1343	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K1060012	.	engineered_region	1331	1343	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K1060012	.	ribosome_entry_site	1337	1340	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K1060012	.	CDS	1350	3085	.	+	0	ID=BBa_J45017;Name=BBa_J45017
BBa_K1060012	.	start_codon	1652	1654	.	+	0	ID=annotation1897454;Name=pchA start codon;Parent=BBa_J45017
BBa_K1060012	.	stop_codon	1653	1655	.	+	0	ID=annotation1897456;Name=pchB stop codon;Parent=BBa_J45017
BBa_K1060012	.	point_mutation	1789	1789	.	+	0	ID=annotation1897453;Name=g -> a to eliminate PstI site;Parent=BBa_J45017
BBa_K1060012	.	CDS	1652	3085	.	+	0	ID=annotation1961297;Name=PchA;Parent=BBa_J45017
BBa_K1060012	.	stop_codon	3080	3085	.	+	0	ID=annotation1897457;Name=pchA double TAA stop codon;Parent=BBa_J45017
BBa_K1060012	.	CDS	1350	1655	.	+	0	ID=annotation1961296;Name=PchB;Parent=BBa_J45017
BBa_K1060012	.	start_codon	1350	1352	.	+	0	ID=annotation1897455;Name=pchB start codon;Parent=BBa_J45017
BBa_K1060012	.	terminator	1166	1260	.	+	0	ID=BBa_K823017;Name=BBa_K823017
BBa_K1060012	.	terminator	1166	1260	.	+	0	ID=BBa_B0014;Name=BBa_B0014;Parent=BBa_K823017
BBa_K1060012	.	terminator	1166	1206	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0014
BBa_K1060012	.	stop_codon	1199	1199	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1060012	.	polyA_site	1193	1206	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1060012	.	stem_loop	1173	1192	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1060012	.	engineered_region	1166	1206	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1060012	.	terminator	1215	1260	.	+	0	ID=BBa_B0011;Name=BBa_B0011;Parent=BBa_B0014
BBa_K1060012	.	engineered_region	1215	1260	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_K1060012	.	stem_loop	1227	1249	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_K1060012	.	CDS	84	1157	.	+	0	ID=BBa_J45004;Name=BBa_J45004
BBa_K1060012	.	start_codon	84	86	.	+	0	ID=annotation1880519;Name=start codon;Parent=BBa_J45004
BBa_K1060012	.	CDS	84	1157	.	+	0	ID=annotation1880517;Name=BMST1;Parent=BBa_J45004
BBa_K1060012	.	stop_codon	1155	1157	.	+	0	ID=annotation1880520;Name=stop codon;Parent=BBa_J45004
BBa_K1060012	.	ribosome_entry_site	63	77	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_K1060012	.	ribosome_entry_site	63	77	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K1060012	.	engineered_region	63	77	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K1060012	.	ribosome_entry_site	70	74	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K1060012	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K1060012	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K1060012	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K1060012	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K1060012	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K1060012	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
>BBa_K1060012
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagattaaagaggagaaata
ctagatggaagttgttgaagttcttcacatgaatggaggaaatggagacagtagctatgcaaacaattctttggttcag
caaaaggtgattctcatgacaaagccaataactgagcaagccatgattgatctctacagcagcctctttccagaaacct
tatgcattgcagatttgggttgttctttgggagctaacactttcttggtggtctcacagcttgttaaaatagtagaaaa
agaacgaaaaaagcatggttttaagtctccagagttttattttcacttcaatgatcttcctggcaatgattttaataca
ctttttcagtcactgggggcatttcaagaagatttgagaaagcatataggggaaagctttggtccatgttttttcagtg
gagtgcctggttcattttatactagacttttcccttccaaaagtttacattttgtttactcctcctacagtctcatgtg
gctatctcaggtgcctaatgggattgaaaataacaagggaaacatttacatggcaagaacaagccctctaagtgttatt
aaagcatactacaagcaatatgaaatagatttttcaaattttctcaagtaccgttcagaggaattgatgaaaggtggaa
agatggtgttaacactcctaggtagagaaagtgaggatcctactagcaaagaatgctgttacatttgggagcttctagc
catggccctcaataagttggttgaagagggattgataaaagaagagaaagtagatgcattcaatattcctcaatacaca
ccatcaccagcagaagtaaagtacatagttgagaaggaaggatcattcaccattaatcgcttggaaacatcaagagttc
attggaatgcttctaataatgagaagaatggtggttacaatgtgtcaaggtgcatgagagctgtggctgagcctttgct
tgtcagccactttgacaaggaattgatggatttagtgttccacaagtacgaagagattgtttctgattgcatgtccaaa
gagaatactgagtttataaatgtcatcatctccttgaccaaaataaattaatactagagtcacactggctcaccttcgg
gtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattttact
agagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagtcacacaggaaag
tactagatgaaaactcccgaagactgcaccggcctggcggacatccgcgaggccatcgaccggatcgacctggatatcg
tccaggccctcggccgccgcatggactacgtcaaggcggcgtcgcgcttcaaggccagcgaggcggcgattccggcgcc
cgagcgggtcgccgcgatgctccccgagcgcgcccgctgggccgaggaaaacggactcgacgcgcccttcgtcgaggga
ctgttcgcgcagatcatccactggtacatcgccgagcagatcaagtactggcgccagacacggggtgccgcatgagccg
gctggcgcccctgagccagtgcctgcacgccttgcgcggcaccttcgagcgcgccatcggccaggcgcaggcgctcgat
cgtccggtgctggtggcggcatcgttcgagatcgacccattggacccgctacaggtattcggtgcctgggacgaccggc
aaacgccctgcctgtactgggaacagcccgagctggcgttcttcgcctggggctgcgccctggagctgcaaggccacgg
cgaacagcgcttcgcccggatcgaggaaaactggcaattgctctgcgccgacgccgtggtcgagggcccgctggcgccg
cgcctgtgcggcggattccgcttcgatccgcgcggcccgcgcgaggaacactggcaagccttcgccgatgccagcctga
tgctcgccggcatcaccgtgctgcgcgagggcgaacgctaccgggtactctgccaacacctggccaagcccggcgaaga
tgccctggccctggccgcctaccactgctcggcgctactgcgcctgaggcagccggccagacgccggccctcggggccg
accgctggcgcgcagggcgacgcttcggcgcaggagcgcaggcaatgggaagccaaggtgagcgacgcggtaagcagtg
tccgccagggacgcttcggcaaggtcgtgctggcccgcacccaggcccggcctctcggcgacatcgagccgtggcaggt
catcgaacacctgcgtctgcaacatgccgacgcccagctgttcgcctgtcgccgcggcaacgcctgcttcctcggcgcc
tccccggaacgcctggtccgcattcgcgccggcgaggcactcacccatgccctggccgggaccatcgcccgcggcggcg
atgcccaggaagatgcgcggctcggacaggccctgctggacagcgccaaggacaggcacgaacaccagttggtggtgga
ggcgatccgtacggccctggaacccttcagcgaggtgctggaaatccccgatgcgcccggcctgaaacgactggcgcga
gtccagcacctgaacacgccgatccgcgcccgcctcgctgacgcaggcggcatcctgcggctgctacaagcgctgcatc
cgacccccgcggtgggcggctacccacgcagcgcggcgctggactacatccgccagcacgaagggatggaccgcggctg
gtacgccgcgccgctgggctggctcgacggcgaaggcaacggcgatttcctggtggcgctgcgctcggccctgctcacg
ccgggccggggctacctgttcgccggctgcggtctggtaggcgattcggaaccggcccacgagtatcgcgaaacctgcc
ttaagctcagtgccatgcgggaagctctatccgccataggcggcctggacgaagtgcccttgcagcgcggcgtcgccta
ataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtc
ggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata