##gff-version 3
##sequence-region BBa_K1036006 1 4046
BBa_K1036006	.	engineered_region	1167	4046	.	+	0	ID=BBa_K1036005;Name=BBa_K1036005
BBa_K1036006	.	terminator	2034	2074	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K1036005
BBa_K1036006	.	polyA_site	2061	2074	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1036006	.	stop_codon	2067	2067	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1036006	.	stem_loop	2041	2060	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1036006	.	engineered_region	2034	2074	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1036006	.	ribosome_entry_site	1167	1178	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K1036005
BBa_K1036006	.	conserved_region	1171	1174	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1036006	.	engineered_region	1185	1937	.	+	0	ID=BBa_K1036004;Name=BBa_K1036004;Parent=BBa_K1036005
BBa_K1036006	.	CDS	1185	1898	.	+	0	ID=annotation2336904;Name=sfGFP;Parent=BBa_K1036004
BBa_K1036006	.	stop_codon	1932	1937	.	+	0	ID=annotation2336906;Name=double stop codon;Parent=BBa_K1036004
BBa_K1036006	.	tag	1899	1931	.	+	0	ID=annotation2336905;Name=LVA;Parent=BBa_K1036004
BBa_K1036006	.	engineered_region	2083	4046	.	+	0	ID=BBa_K546000;Name=BBa_K546000;Parent=BBa_K1036005
BBa_K1036006	.	engineered_region	3249	4046	.	+	0	ID=BBa_K082014;Name=BBa_K082014;Parent=BBa_K546000
BBa_K1036006	.	terminator	3918	4046	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K082014
BBa_K1036006	.	terminator	4006	4046	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1036006	.	polyA_site	4033	4046	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1036006	.	stop_codon	4039	4039	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1036006	.	stem_loop	4013	4032	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1036006	.	engineered_region	4006	4046	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1036006	.	terminator	3918	3997	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1036006	.	stem_loop	3929	3972	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1036006	.	engineered_region	3918	3997	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1036006	.	engineered_region	3249	3909	.	+	0	ID=BBa_C0261;Name=BBa_C0261;Parent=BBa_K082014
BBa_K1036006	.	ribosome_entry_site	3249	3260	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_C0261
BBa_K1036006	.	conserved_region	3253	3256	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1036006	.	CDS	3267	3884	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_C0261
BBa_K1036006	.	tag	3846	3877	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K1036006	.	engineered_region	3267	3884	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K1036006	.	engineered_tag	3885	3909	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K1036006	.	CDS	3267	3845	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K1036006	.	engineered_region	2083	3240	.	+	0	ID=BBa_F2621;Name=BBa_F2621;Parent=BBa_K546000
BBa_K1036006	.	terminator	3049	3128	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_F2621
BBa_K1036006	.	stem_loop	3060	3103	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1036006	.	engineered_region	3049	3128	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1036006	.	terminator	3137	3177	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_F2621
BBa_K1036006	.	polyA_site	3164	3177	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1036006	.	stop_codon	3170	3170	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1036006	.	stem_loop	3144	3163	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1036006	.	engineered_region	3137	3177	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1036006	.	promoter	3186	3240	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_F2621
BBa_K1036006	.	non_covalent_binding_site	3186	3205	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K1036006	.	start_codon	3238	3238	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K1036006	.	promoter	3205	3210	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K1036006	.	promoter	3227	3232	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K1036006	.	engineered_region	3186	3240	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K1036006	.	ribosome_entry_site	2242	2253	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_F2621
BBa_K1036006	.	conserved_region	2246	2249	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1036006	.	CDS	2260	3015	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_F2621
BBa_K1036006	.	engineered_region	2260	3015	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K1036006	.	engineered_tag	3016	3040	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K1036006	.	engineered_region	2260	2261	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K1036006	.	point_mutation	2433	2433	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K1036006	.	point_mutation	2751	2751	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K1036006	.	CDS	2260	3009	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K1036006	.	promoter	2083	2233	.	+	0	ID=BBa_R0063;Name=BBa_R0063;Parent=BBa_F2621
BBa_K1036006	.	promoter	2171	2176	.	+	0	ID=annotation2053;Name=-35;Parent=BBa_R0063
BBa_K1036006	.	promoter	2197	2204	.	+	0	ID=annotation2052;Name=-10;Parent=BBa_R0063
BBa_K1036006	.	non_covalent_binding_site	2212	2231	.	+	0	ID=annotation2055;Name=Putative LuxR/HSL;Parent=BBa_R0063
BBa_K1036006	.	engineered_region	2083	2233	.	+	0	ID=annotation7071;Name=BBa_R0063;Parent=BBa_R0063
BBa_K1036006	.	non_covalent_binding_site	2083	2102	.	+	0	ID=annotation2051;Name=LuxR/HSL;Parent=BBa_R0063
BBa_K1036006	.	start_codon	2210	2210	.	+	0	ID=annotation2054;Name=start;Parent=BBa_R0063
BBa_K1036006	.	terminator	1946	2025	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K1036005
BBa_K1036006	.	stem_loop	1957	2000	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1036006	.	engineered_region	1946	2025	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1036006	.	engineered_region	1	1158	.	+	0	ID=BBa_F2621;Name=BBa_F2621
BBa_K1036006	.	terminator	967	1046	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_F2621
BBa_K1036006	.	stem_loop	978	1021	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1036006	.	engineered_region	967	1046	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1036006	.	terminator	1055	1095	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_F2621
BBa_K1036006	.	polyA_site	1082	1095	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1036006	.	stop_codon	1088	1088	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1036006	.	stem_loop	1062	1081	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1036006	.	engineered_region	1055	1095	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1036006	.	promoter	1104	1158	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_F2621
BBa_K1036006	.	non_covalent_binding_site	1104	1123	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K1036006	.	start_codon	1156	1156	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K1036006	.	promoter	1123	1128	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K1036006	.	promoter	1145	1150	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K1036006	.	engineered_region	1104	1158	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K1036006	.	ribosome_entry_site	160	171	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_F2621
BBa_K1036006	.	conserved_region	164	167	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1036006	.	CDS	178	933	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_F2621
BBa_K1036006	.	engineered_region	178	933	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K1036006	.	engineered_tag	934	958	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K1036006	.	engineered_region	178	179	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K1036006	.	point_mutation	351	351	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K1036006	.	point_mutation	669	669	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K1036006	.	CDS	178	927	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K1036006	.	promoter	1	151	.	+	0	ID=BBa_R0063;Name=BBa_R0063;Parent=BBa_F2621
BBa_K1036006	.	promoter	89	94	.	+	0	ID=annotation2053;Name=-35;Parent=BBa_R0063
BBa_K1036006	.	promoter	115	122	.	+	0	ID=annotation2052;Name=-10;Parent=BBa_R0063
BBa_K1036006	.	non_covalent_binding_site	130	149	.	+	0	ID=annotation2055;Name=Putative LuxR/HSL;Parent=BBa_R0063
BBa_K1036006	.	engineered_region	1	151	.	+	0	ID=annotation7071;Name=BBa_R0063;Parent=BBa_R0063
BBa_K1036006	.	non_covalent_binding_site	1	20	.	+	0	ID=annotation2051;Name=LuxR/HSL;Parent=BBa_R0063
BBa_K1036006	.	start_codon	128	128	.	+	0	ID=annotation2054;Name=start;Parent=BBa_R0063
BBa_K1036006	.	engineered_region	2083	3240	.	+	0	ID=BBa_F2621;Name=BBa_F2621
BBa_K1036006	.	terminator	3049	3128	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_F2621
BBa_K1036006	.	stem_loop	3060	3103	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1036006	.	engineered_region	3049	3128	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1036006	.	terminator	3137	3177	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_F2621
BBa_K1036006	.	polyA_site	3164	3177	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1036006	.	stop_codon	3170	3170	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1036006	.	stem_loop	3144	3163	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1036006	.	engineered_region	3137	3177	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1036006	.	promoter	3186	3240	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_F2621
BBa_K1036006	.	non_covalent_binding_site	3186	3205	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K1036006	.	start_codon	3238	3238	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K1036006	.	promoter	3205	3210	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K1036006	.	promoter	3227	3232	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K1036006	.	engineered_region	3186	3240	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K1036006	.	ribosome_entry_site	2242	2253	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_F2621
BBa_K1036006	.	conserved_region	2246	2249	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1036006	.	CDS	2260	3015	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_F2621
BBa_K1036006	.	engineered_region	2260	3015	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K1036006	.	engineered_tag	3016	3040	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K1036006	.	engineered_region	2260	2261	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K1036006	.	point_mutation	2433	2433	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K1036006	.	point_mutation	2751	2751	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K1036006	.	CDS	2260	3009	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K1036006	.	promoter	2083	2233	.	+	0	ID=BBa_R0063;Name=BBa_R0063;Parent=BBa_F2621
BBa_K1036006	.	promoter	2171	2176	.	+	0	ID=annotation2053;Name=-35;Parent=BBa_R0063
BBa_K1036006	.	promoter	2197	2204	.	+	0	ID=annotation2052;Name=-10;Parent=BBa_R0063
BBa_K1036006	.	non_covalent_binding_site	2212	2231	.	+	0	ID=annotation2055;Name=Putative LuxR/HSL;Parent=BBa_R0063
BBa_K1036006	.	engineered_region	2083	2233	.	+	0	ID=annotation7071;Name=BBa_R0063;Parent=BBa_R0063
BBa_K1036006	.	non_covalent_binding_site	2083	2102	.	+	0	ID=annotation2051;Name=LuxR/HSL;Parent=BBa_R0063
BBa_K1036006	.	start_codon	2210	2210	.	+	0	ID=annotation2054;Name=start;Parent=BBa_R0063
>BBa_K1036006
acctgtacgatcctacaggtgcttatgttaagtaattgtattcccagcgatacaatagtgtgacaaaaatccaatttat
tagaatcaaatgtcaatccattaccgttttaatgatatataacacgcaaaacttgcgacaaacaataggtaatactaga
gaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtaga
agcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcattt
atcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgc
taatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaac
aatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttcccta
ttcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttattttt
acatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaa
tcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaa
aaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctg
ccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctag
tgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttg
tttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacc
tgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatactaga
tgcgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagtt
ttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactg
ccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagc
agcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgta
caaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaa
gacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtttacatcaccgccgataaacaaaaaa
atggcattaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaa
cactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccg
aacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtaca
aagctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagt
cgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcggg
tgggcctttctgcgtttatatactagagacctgtacgatcctacaggtgcttatgttaagtaattgtattcccagcgat
acaatagtgtgacaaaaatccaatttattagaatcaaatgtcaatccattaccgttttaatgatatataacacgcaaaa
cttgcgacaaacaataggtaatactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacag
aataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacat
tgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattacccta
aaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattc
accaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatca
ggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaa
aagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataatta
tcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgc
gaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgc
aaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactt
taaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcga
aagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgg
gcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataa
atactagagaaagaggagaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagt
ataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttga
atcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgt
ttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatc
ctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaat
gaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaata
gagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaat
cggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgcttt
agtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcga
aagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgg
gcctttctgcgtttata