##gff-version 3
##sequence-region BBa_K1036002 1 2886
BBa_K1036002	.	engineered_region	923	2886	.	+	0	ID=BBa_K546000;Name=BBa_K546000
BBa_K1036002	.	engineered_region	2089	2886	.	+	0	ID=BBa_K082014;Name=BBa_K082014;Parent=BBa_K546000
BBa_K1036002	.	engineered_region	2089	2749	.	+	0	ID=BBa_C0261;Name=BBa_C0261;Parent=BBa_K082014
BBa_K1036002	.	CDS	2107	2724	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_C0261
BBa_K1036002	.	CDS	2107	2685	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K1036002	.	engineered_region	2107	2724	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K1036002	.	engineered_tag	2725	2749	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K1036002	.	tag	2686	2717	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K1036002	.	ribosome_entry_site	2089	2100	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_C0261
BBa_K1036002	.	conserved_region	2093	2096	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1036002	.	terminator	2758	2886	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K082014
BBa_K1036002	.	terminator	2846	2886	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1036002	.	polyA_site	2873	2886	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1036002	.	stem_loop	2853	2872	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1036002	.	stop_codon	2879	2879	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1036002	.	engineered_region	2846	2886	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1036002	.	terminator	2758	2837	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1036002	.	stem_loop	2769	2812	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1036002	.	engineered_region	2758	2837	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1036002	.	engineered_region	923	2080	.	+	0	ID=BBa_F2621;Name=BBa_F2621;Parent=BBa_K546000
BBa_K1036002	.	terminator	1977	2017	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_F2621
BBa_K1036002	.	polyA_site	2004	2017	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1036002	.	stem_loop	1984	2003	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1036002	.	stop_codon	2010	2010	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1036002	.	engineered_region	1977	2017	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1036002	.	ribosome_entry_site	1082	1093	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_F2621
BBa_K1036002	.	conserved_region	1086	1089	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1036002	.	promoter	2026	2080	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_F2621
BBa_K1036002	.	start_codon	2078	2078	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K1036002	.	promoter	2045	2050	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K1036002	.	promoter	2067	2072	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K1036002	.	engineered_region	2026	2080	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K1036002	.	non_covalent_binding_site	2026	2045	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K1036002	.	terminator	1889	1968	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_F2621
BBa_K1036002	.	stem_loop	1900	1943	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1036002	.	engineered_region	1889	1968	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1036002	.	CDS	1100	1855	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_F2621
BBa_K1036002	.	CDS	1100	1849	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K1036002	.	point_mutation	1591	1591	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K1036002	.	engineered_tag	1856	1880	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K1036002	.	point_mutation	1273	1273	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K1036002	.	engineered_region	1100	1101	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K1036002	.	engineered_region	1100	1855	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K1036002	.	promoter	923	1073	.	+	0	ID=BBa_R0063;Name=BBa_R0063;Parent=BBa_F2621
BBa_K1036002	.	non_covalent_binding_site	923	942	.	+	0	ID=annotation2051;Name=LuxR/HSL;Parent=BBa_R0063
BBa_K1036002	.	promoter	1037	1044	.	+	0	ID=annotation2052;Name=-10;Parent=BBa_R0063
BBa_K1036002	.	engineered_region	923	1073	.	+	0	ID=annotation7071;Name=BBa_R0063;Parent=BBa_R0063
BBa_K1036002	.	start_codon	1050	1050	.	+	0	ID=annotation2054;Name=start;Parent=BBa_R0063
BBa_K1036002	.	non_covalent_binding_site	1052	1071	.	+	0	ID=annotation2055;Name=Putative LuxR/HSL;Parent=BBa_R0063
BBa_K1036002	.	promoter	1011	1016	.	+	0	ID=annotation2053;Name=-35;Parent=BBa_R0063
BBa_K1036002	.	engineered_region	1	914	.	+	0	ID=BBa_I763020;Name=BBa_I763020
BBa_K1036002	.	terminator	786	865	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I763020
BBa_K1036002	.	stem_loop	797	840	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1036002	.	engineered_region	786	865	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1036002	.	engineered_region	19	777	.	+	0	ID=BBa_J04031;Name=BBa_J04031;Parent=BBa_I763020
BBa_K1036002	.	start_codon	19	21	.	+	0	ID=annotation1507246;Name=Start;Parent=BBa_J04031
BBa_K1036002	.	tag	733	771	.	+	0	ID=annotation1507235;Name=LVA;Parent=BBa_J04031
BBa_K1036002	.	engineered_region	19	738	.	+	0	ID=annotation1507202;Name=BBa_E0040;Parent=BBa_J04031
BBa_K1036002	.	stop_codon	772	777	.	+	0	ID=annotation1507242;Name=Double Stop;Parent=BBa_J04031
BBa_K1036002	.	terminator	874	914	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I763020
BBa_K1036002	.	polyA_site	901	914	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1036002	.	stem_loop	881	900	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1036002	.	stop_codon	907	907	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1036002	.	engineered_region	874	914	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1036002	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I763020
BBa_K1036002	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_K1036002
aaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtg
atgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttat
ttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgaga
tacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttt
tcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaa
aggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatc
atggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactag
cagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatc
tgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacat
ggcatggatgaactatacaaaaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccagg
catcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctact
agagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtacgatcctacaggtgcttat
gttaagtaattgtattcccagcgatacaatagtgtgacaaaaatccaatttattagaatcaaatgtcaatccattaccg
ttttaatgatatataacacgcaaaacttgcgacaaacaataggtaatactagagaaagaggagaaatactagatgaaaa
acataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgctt
atctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgat
atttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatag
tagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaa
tgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcgga
atgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaa
ttgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagaga
aaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtact
gtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaa
caggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcat
caaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactaga
gtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaag
aaaatggtttgttatagtcgaataaatactagagaaagaggagaaatactagatgactataatgataaaaaaatcggat
tttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtggg
acttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatac
tgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgctt
ggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataa
ataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaata
tgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaa
attcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatg
ctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcat
caaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactaga
gtcacactggctcaccttcgggtgggcctttctgcgtttata