##gff-version 3
##sequence-region BBa_K101004 1 587
BBa_K101004	.	ribosome_entry_site	209	221	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_K101004	.	engineered_region	209	221	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K101004	.	ribosome_entry_site	209	221	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K101004	.	ribosome_entry_site	215	218	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K101004	.	CDS	228	540	.	+	0	ID=BBa_C0072;Name=BBa_C0072
BBa_K101004	.	CDS	228	476	.	+	0	ID=annotation308499;Name=mnt;Parent=BBa_C0072
BBa_K101004	.	engineered_tag	516	540	.	+	0	ID=annotation2214010;Name=Help:Barcodes;Parent=BBa_C0072
BBa_K101004	.	point_mutation	322	324	.	+	0	ID=annotation308502;Name=T;Parent=BBa_C0072
BBa_K101004	.	tag	477	509	.	+	0	ID=annotation308503;Name=LVA;Parent=BBa_C0072
BBa_K101004	.	terminator	549	587	.	+	0	ID=BBa_B1006;Name=BBa_B1006
BBa_K101004	.	polyA_site	549	557	.	+	0	ID=annotation1898431;Name=PolyA;Parent=BBa_B1006
BBa_K101004	.	stem_loop	558	579	.	+	0	ID=annotation1898429;Name=modified thr terminator;Parent=BBa_B1006
BBa_K101004	.	polyA_site	580	587	.	+	0	ID=annotation1898430;Name=PolyA;Parent=BBa_B1006
BBa_K101004	.	engineered_region	549	587	.	+	0	ID=annotation1898428;Name=B1006;Parent=BBa_B1006
BBa_K101004	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_K101004	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K101004	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K101004	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K101004	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K101004	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K101004	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K101004	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
>BBa_K101004
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagtcacacaggaaagtactagatggcccggg
atgatcctcacttcaattttcgtatgccaatggaagtaagagagaaattgaaatttagagcagaggcaaacggacggag
catgaactctgagcttttgcaaatcgtacaagatgccctaagcaaaccgtcaccagtcactgggtaccgcaatgatgcg
gaacgactcgccgatgagcagagcgagttagtgaagaagatggtcttcgatacactgaaggatctttataaaaaaacca
ccgctgcaaacgacgaaaactacgctttagtagcttaataaccctgatagtgctagtgtagatccctactagagaaaaa
aaaaccccgcccctgacagggcggggtttttttt