##gff-version 3
##sequence-region BBa_K092700 1 2414
BBa_K092700	.	engineered_region	1	1634	.	+	0	ID=BBa_K092600;Name=BBa_K092600
BBa_K092700	.	engineered_region	849	1634	.	+	0	ID=BBa_K092300;Name=BBa_K092300;Parent=BBa_K092600
BBa_K092700	.	ribosome_entry_site	911	922	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K092300
BBa_K092700	.	conserved_region	915	918	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K092700	.	promoter	849	902	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_K092300
BBa_K092700	.	engineered_region	849	902	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K092700	.	promoter	868	873	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K092700	.	promoter	891	896	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K092700	.	non_covalent_binding_site	849	867	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K092700	.	non_covalent_binding_site	874	892	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K092700	.	CDS	929	1634	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_K092300
BBa_K092700	.	engineered_tag	1610	1634	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K092700	.	CDS	929	1603	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K092700	.	engineered_region	1	840	.	+	0	ID=BBa_K092000;Name=BBa_K092000;Parent=BBa_K092600
BBa_K092700	.	terminator	712	840	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K092000
BBa_K092700	.	terminator	712	791	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K092700	.	engineered_region	712	791	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K092700	.	stem_loop	723	766	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K092700	.	terminator	800	840	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K092700	.	stop_codon	833	833	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K092700	.	polyA_site	827	840	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K092700	.	stem_loop	807	826	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K092700	.	engineered_region	800	840	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K092700	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K092000
BBa_K092700	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K092700	.	CDS	19	703	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_K092000
BBa_K092700	.	engineered_tag	679	703	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K092700	.	CDS	22	638	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K092700	.	tag	639	672	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K092700	.	engineered_region	1643	2414	.	+	0	ID=BBa_K092400;Name=BBa_K092400
BBa_K092700	.	CDS	1668	2249	.	+	0	ID=annotation1982736;Name=LuxI;Parent=BBa_K092400
BBa_K092700	.	engineered_region	2286	2414	.	+	0	ID=annotation1982737;Name=B0015 Terminator;Parent=BBa_K092400
BBa_K092700	.	engineered_tag	2250	2285	.	+	0	ID=annotation1982738;Name=barcode;Parent=BBa_K092400
BBa_K092700	.	engineered_region	1650	1661	.	+	0	ID=annotation1982735;Name=B0034 RBS;Parent=BBa_K092400
>BBa_K092700
aaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcg
gaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaa
taagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaa
agctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtac
atttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttc
actagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaa
gtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatc
accaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaag
tgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagag
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct
ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagatt
gacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatggcttcctccgaagacgt
tatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggt
gaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggaca
tcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtc
cttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctcc
ctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaa
aaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtct
gaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggt
gcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctg
aaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagcttgcataaagaggaga
aatactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtct
tcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataac
tcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtg
attatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaag
tcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctata
tataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgta
ttaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcc
tattaatgaacagtttaaaaaagcagtcttaaattaataacgctgatagtgctagtgtagatcgctactagagccaggc
atcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctacta
gagtcacactggctcaccttcgggtgggcctttctgcgtttata