##gff-version 3
##sequence-region BBa_K091118 1 1985
BBa_K091118	.	promoter	945	1101	.	+	0	ID=BBa_R0079;Name=BBa_R0079
BBa_K091118	.	non_covalent_binding_site	990	1007	.	+	0	ID=annotation300985;Name=OP2;Parent=BBa_R0079
BBa_K091118	.	promoter	1061	1066	.	+	0	ID=annotation318495;Name=-35;Parent=BBa_R0079
BBa_K091118	.	promoter	1084	1089	.	+	0	ID=annotation318496;Name=-10;Parent=BBa_R0079
BBa_K091118	.	non_covalent_binding_site	1050	1069	.	+	0	ID=annotation300992;Name=OP1;Parent=BBa_R0079
BBa_K091118	.	engineered_region	1110	1985	.	+	0	ID=BBa_E0240;Name=BBa_E0240
BBa_K091118	.	terminator	1857	1936	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0240
BBa_K091118	.	stem_loop	1868	1911	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K091118	.	engineered_region	1857	1936	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K091118	.	ribosome_entry_site	1110	1122	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_E0240
BBa_K091118	.	engineered_region	1110	1122	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K091118	.	ribosome_entry_site	1110	1122	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K091118	.	ribosome_entry_site	1116	1119	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K091118	.	CDS	1129	1848	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_E0240
BBa_K091118	.	CDS	1129	1848	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K091118	.	terminator	1945	1985	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0240
BBa_K091118	.	polyA_site	1972	1985	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K091118	.	engineered_region	1945	1985	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K091118	.	stem_loop	1952	1971	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K091118	.	stop_codon	1978	1978	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K091118	.	terminator	808	936	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K091118	.	terminator	896	936	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K091118	.	polyA_site	923	936	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K091118	.	engineered_region	896	936	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K091118	.	stem_loop	903	922	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K091118	.	stop_codon	929	929	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K091118	.	terminator	808	887	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K091118	.	stem_loop	819	862	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K091118	.	engineered_region	808	887	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K091118	.	engineered_region	1	799	.	+	0	ID=BBa_S03156;Name=BBa_S03156
BBa_K091118	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S03156
BBa_K091118	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K091118	.	CDS	19	774	.	+	0	ID=BBa_C0079;Name=BBa_C0079;Parent=BBa_S03156
BBa_K091118	.	engineered_tag	775	799	.	+	0	ID=annotation2213999;Name=Help:Barcodes;Parent=BBa_C0079
BBa_K091118	.	tag	736	768	.	+	0	ID=annotation307948;Name=LVA;Parent=BBa_C0079
BBa_K091118	.	CDS	19	735	.	+	0	ID=annotation307935;Name=lasR;Parent=BBa_C0079
BBa_K091118	.	sequence_alteration	498	498	.	+	0	ID=annotation308006;Name=G;Parent=BBa_C0079
>BBa_K091118
aaagaggagaaatactagatggccttggttgacggttttcttgagctggaacgctcaagtggaaaattggagtggagcg
ccatcctccagaagatggcgagcgaccttggattctcgaagatcctgttcggcctgttgcctaaggacagccaggacta
cgagaacgccttcatcgtcggcaactacccggccgcctggcgcgagcattacgaccgggctggctacgcgcgggtcgac
ccgacggtcagtcactgtacccagagcgtactgccgattttctgggaaccgtccatctaccagacgcgaaagcagcacg
agttcttcgaggaagcctcggccgccggcctggtgtatgggctgaccatgccgctgcatggtgctcgcggcgaactcgg
cgcgctgagcctcagcgtggaagcggaaaaccgggccgaggccaaccgtttcatagagtcggtcctgccgaccctgtgg
atgctcaaggactacgcactgcaaagcggtgccggactggccttcgaacatccggtcagcaaaccggtggttctgacca
gccgggagaaggaagtgttgcagtggtgcgccatcggcaagaccagttgggagatatcggttatctgcaactgctcgga
agccaatgtgaacttccatatgggaaatattcggcggaagttcggtgtgacctcccgccgcgtagcggccattatggcc
gttaatttgggtcttattactctcgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagt
gtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgt
ttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggccc
ctcgctgagcgcgtcccggagctgggggcaacctagctgccacctgcttttctgctagctattccagcgaaaacataca
gatttccggcgaaatcaaggctacctgccagttctggcaggtttggccgcgggttctttttggtacacgaaagctacta
gagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagat
ggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaat
ttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgc
gagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactata
tttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagt
taaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtata
catcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaa
ctagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacac
aatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattac
acatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactg
ggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttc
tgcgtttata