##gff-version 3 ##sequence-region BBa_J69211 1 243 BBa_J69211 . CDS 84 89 . + 0 ID=BBa_C0099;Name=BBa_C0099 BBa_J69211 . ribosome_entry_site 63 77 . + 0 ID=BBa_B0030;Name=BBa_B0030 BBa_J69211 . ribosome_entry_site 63 77 . + 0 ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030 BBa_J69211 . engineered_region 63 77 . + 0 ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030 BBa_J69211 . ribosome_entry_site 70 74 . + 0 ID=annotation1702;Name=RBS;Parent=BBa_B0030 BBa_J69211 . promoter 235 243 . + 0 ID=BBa_R0099;Name=BBa_R0099 BBa_J69211 . terminator 186 226 . + 0 ID=BBa_B0012;Name=BBa_B0012 BBa_J69211 . stop_codon 219 219 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_J69211 . polyA_site 213 226 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_J69211 . stem_loop 193 212 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_J69211 . engineered_region 186 226 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_J69211 . terminator 98 177 . + 0 ID=BBa_B0010;Name=BBa_B0010 BBa_J69211 . stem_loop 109 152 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 BBa_J69211 . engineered_region 98 177 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 BBa_J69211 . promoter 1 54 . + 0 ID=BBa_R0040;Name=BBa_R0040 BBa_J69211 . non_covalent_binding_site 1 19 . + 0 ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040 BBa_J69211 . non_covalent_binding_site 26 44 . + 0 ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040 BBa_J69211 . engineered_region 1 54 . + 0 ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040 BBa_J69211 . promoter 20 25 . + 0 ID=annotation1986785;Name=-35;Parent=BBa_R0040 BBa_J69211 . promoter 43 48 . + 0 ID=annotation1986787;Name=-10;Parent=BBa_R0040 >BBa_J69211 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagattaaagaggagaaata ctagatgatgtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttg tttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagcgt cgtcgt