##gff-version 3
##sequence-region BBa_J69107 1 2645
BBa_J69107	.	engineered_region	1	1768	.	+	0	ID=BBa_J28032;Name=BBa_J28032
BBa_J69107	.	CDS	1127	1768	.	+	0	ID=annotation1904959;Name=BBa_C0078 (lasI), codons optimised for E. coli;Parent=BBa_J28032
BBa_J69107	.	stem_loop	1	129	.	+	0	ID=annotation1904949;Name=BBa_B0015;Parent=BBa_J28032
BBa_J69107	.	CDS	138	860	.	+	0	ID=annotation1904951;Name=BBa_C0079 (lasR), codons optimised for E. coli;Parent=BBa_J28032
BBa_J69107	.	ribosome_entry_site	1109	1120	.	+	0	ID=annotation1904958;Name=BBa_B0034;Parent=BBa_J28032
BBa_J69107	.	promoter	944	1100	.	+	0	ID=annotation1904956;Name=BBa_R0079 (las pR);Parent=BBa_J28032
BBa_J69107	.	sequence_feature	880	885	.	+	0	ID=annotation1904953;Name=BamHI restriction site;Parent=BBa_J28032
BBa_J69107	.	ribosome_entry_site	867	878	.	+	0	ID=annotation1904952;Name=BBa_B0034;Parent=BBa_J28032
BBa_J69107	.	promoter	887	935	.	+	0	ID=annotation1904954;Name=BBa_R0051 (lamda cI);Parent=BBa_J28032
BBa_J69107	.	sequence_feature	937	942	.	+	0	ID=annotation1904955;Name=BspHI restriction site;Parent=BBa_J28032
BBa_J69107	.	sequence_feature	131	136	.	+	0	ID=annotation1904950;Name=SphI restriction site;Parent=BBa_J28032
BBa_J69107	.	engineered_region	1	1768	.	+	0	ID=annotation1904960;Name=BBa_J28032;Parent=BBa_J28032
BBa_J69107	.	sequence_feature	1102	1107	.	+	0	ID=annotation1904957;Name=SalI restriction site;Parent=BBa_J28032
BBa_J69107	.	engineered_region	1795	2508	.	+	0	ID=BBa_J06504;Name=BBa_J06504
BBa_J69107	.	sequence_alteration	2146	2146	.	+	0	ID=annotation1585833;Name=C->T (removing PstI site);Parent=BBa_J06504
BBa_J69107	.	CDS	1795	2505	.	+	0	ID=annotation1585829;Name=mCherry;Parent=BBa_J06504
BBa_J69107	.	terminator	2605	2645	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J69107	.	engineered_region	2605	2645	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J69107	.	polyA_site	2632	2645	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J69107	.	stem_loop	2612	2631	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J69107	.	stop_codon	2638	2638	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J69107	.	ribosome_entry_site	1777	1788	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J69107	.	conserved_region	1781	1784	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J69107	.	terminator	2517	2596	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J69107	.	engineered_region	2517	2596	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J69107	.	stem_loop	2528	2571	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
>BBa_J69107
tataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaa
acgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggtgcatgcgttattacagagtaatcaggcc
caggttaacggccataatcgctgcaacgcggcgagaagtaacaccgaatttacgacgaatgttacccatgtggaagttg
acgttggcttcagagcagttgcaaattacagaaatctcccaggaggttttgccgatagcgcaccattgcaggacctctt
tttcacggctggtcagaactaccggtttagaaactgggtgttcgaacgccagacccgcgccggattgcagtgcataatc
tttcagcatccacagggtcggcagtacagattcgatgaaacggttagcctccgcgcggttttctgcttctacggacaga
gacagagcgcccagttcgccacgagcaccatgcagcggcatcgtcaggccgtaaaccagaccagccgcggacgcctctt
cgaagaactcgtgctgtttacgggtctggtagatgctcggttcccagaaaatcggcagcacggactgggtgcaatggga
aacggttggatctacacgtgcataaccggcacgatcataatgttcacgccacgcggccgggtaattgcctacgatgaat
gcgttttcgtagtcttgggaatctttcggcagcaggccgaacaggattttagagaaacccaggtcagaggccatttttt
gcaggatagcgctccattccagtttaccagaagaacgttccagttccagaaaaccatccaccagagccatctagtattt
ctcctcttttggatccggcaaccattatcaccgccagaggtaaaatagtcaacacgcacggtgttattcatgaggcccc
tcgctgagcgcgtcccggagctgggggcaacctagctgccacctgcttttctgctagctattccagcgaaaacatacag
atttccggcgaaatcaaggctacctgccagttctggcaggtttggccgcgggttctttttggtacacgaaagctgtcga
cgaaagaggagaaatactagatgattgttcagattggccgtcgtgaggaatttgataaaaagctgctgggcgagatgca
caaactgcgtgcacaggttttcaaggagcgtaaaggttgggacgttagcgttattgacgaaatggagattgatggctac
gacgctctgtctccgtattatatgctgattcaggaggacaccccggaagctcaggtatttggttgctggcgtatcttcg
atactacgggtccgtatatgctgaaaaataccttcccggaactgctgcacggtaaagaagcgccttgtagcccgcacat
ctgggaactgtctcgtttcgctatcaactccggccagaaaggttccctgggcttttccgattgtaccctggaagcaatg
cgtgcgctggcgcgctattccctgcaaaacgacatccagactctggtgaccgttactactgtgggcgttgaaaagatga
tgatccgtgcaggcctggacgttagccgtttcggtccacacctgaagatcggtatcgaacgcgcggtggccctgcgtat
cgaactgaatgcaaaaacccagatcgcactgtatggtggtgttctggtggaacaacgcctggccgtttccgcggctaat
gatgagaactacgcgctggtagcataataatactagagaaagaggagaaatactagatggtgagcaagggcgaggagga
taacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatc
gagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgccct
tcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgacta
cttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacc
caggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccg
taatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagat
caagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtg
cagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagt
acgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtaataatactagagccaggcatcaaa
taaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtca
cactggctcaccttcgggtgggcctttctgcgtttata