##gff-version 3
##sequence-region BBa_J69007 1 2558
BBa_J69007	.	engineered_region	1	1681	.	+	0	ID=BBa_J28031;Name=BBa_J28031
BBa_J69007	.	ribosome_entry_site	1046	1057	.	+	0	ID=annotation1904935;Name=BBa_B0034;Parent=BBa_J28031
BBa_J69007	.	promoter	983	1037	.	+	0	ID=annotation1904933;Name=BBa_R0062 (lux pR);Parent=BBa_J28031
BBa_J69007	.	sequence_feature	131	136	.	+	0	ID=annotation1904927;Name=SphI restriction site;Parent=BBa_J28031
BBa_J69007	.	sequence_feature	1039	1044	.	+	0	ID=annotation1904934;Name=SalI restriction site;Parent=BBa_J28031
BBa_J69007	.	sequence_feature	913	918	.	+	0	ID=annotation1904930;Name=BamHI restriction site;Parent=BBa_J28031
BBa_J69007	.	CDS	138	893	.	+	0	ID=annotation1904928;Name=BBa_C0062 (luxR), codons optimised for E. coli;Parent=BBa_J28031
BBa_J69007	.	engineered_region	1	1681	.	+	0	ID=annotation1904937;Name=BBa_J28031;Parent=BBa_J28031
BBa_J69007	.	promoter	920	974	.	+	0	ID=annotation1904931;Name=BBa_R0011 (lac, lambda cI hybrid);Parent=BBa_J28031
BBa_J69007	.	sequence_feature	976	981	.	+	0	ID=annotation1904932;Name=BspHI restriction site;Parent=BBa_J28031
BBa_J69007	.	CDS	1064	1681	.	+	0	ID=annotation1904936;Name=BBa_C0061 (luxI), codons optimised for E. coli;Parent=BBa_J28031
BBa_J69007	.	stem_loop	1	129	.	+	0	ID=annotation1904926;Name=BBa_B0015;Parent=BBa_J28031
BBa_J69007	.	ribosome_entry_site	900	911	.	+	0	ID=annotation1904929;Name=BBa_B0034;Parent=BBa_J28031
BBa_J69007	.	terminator	2518	2558	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J69007	.	stop_codon	2551	2551	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J69007	.	stem_loop	2525	2544	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J69007	.	engineered_region	2518	2558	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J69007	.	polyA_site	2545	2558	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J69007	.	terminator	2430	2509	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J69007	.	stem_loop	2441	2484	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J69007	.	engineered_region	2430	2509	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J69007	.	ribosome_entry_site	1690	1701	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J69007	.	conserved_region	1694	1697	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J69007	.	engineered_region	1708	2421	.	+	0	ID=BBa_J06504;Name=BBa_J06504
BBa_J69007	.	sequence_alteration	2059	2059	.	+	0	ID=annotation1585833;Name=C->T (removing PstI site);Parent=BBa_J06504
BBa_J69007	.	CDS	1708	2418	.	+	0	ID=annotation1585829;Name=mCherry;Parent=BBa_J06504
>BBa_J69007
tataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaa
acgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggtgcatgcgttattagtttttgaaatacgg
gcaatcgatcgcaccggtcaggatagctttgctgatagattgacaacgattcgtggtgttcagtttcatctgcgcgttg
gtcagatggaaagttacggtgcgttcagagcaacccagaattttggagatgtcccaagaggacttaccttcgcacgccc
atgccaggcattctttctcgcgtttggtcaggtcgttgttgcttttgttattggcaatgttaatcttgcgatagttatc
aaccagggacggaacgatcagaggaatgttcatgcacgcgtgcaggaacagagaatcgatgtagttgtctttttcggag
tgcgcaaaagacagcataccgaagccgttgttcgcggtatgaatcgggaagctgaaaccggtgatcagaccgctggttt
ttgcttctttgataacgtttggggactttttgttaaccgcgttgttctcaaagatattccagttaatcggggaatggtt
agagttagagtaatctacgatcggatcgtacttgatcaggttggcgtcatcatagtactgacgccatttttttgggtag
ttatccagaatagaaatatcggattttaccatgctgtgcggataaatgatggccagcagataatattcacagtgaacca
ttttggtcatatcagacaggcattggttaatatcgttgttagaacgacacgctttgattttgttaatgatacggtaggt
gtcgtccgcgttgatgtttttcatctagtatttctcctcttttggatccgtgtgctcagtatcttgttatccgctcaca
atgtcaattgttatccgctcacaattttcatgagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttata
gtcgaataaatgtcgacgaaagaggagaaatactagatgaccattatgatcaagaaatctgacttcctggccatcccgt
ccgaagaatacaaaggcatcctgtctctgcgctaccaggttttcaaacagcgtctggaatgggacctggtggttgaaaa
caacctggaatctgacgagtatgataattccaacgcagaatacatctatgcgtgtgatgatactgaaaacgtttctggt
tgttggcgtctgctgcctaccacgggcgattatatgctgaagtccgtatttccggagctgctgggtcagcagtccgcgc
cgaaagacccgaacattgtagaactgtctcgcttcgcggttggcaagaacagctctaaaatcaacaactccgcaagcga
aattaccatgaaactgttcgaagctatttataaacacgcggtttcccagggtatcaccgaatacgtgaccgtcactagc
accgctatcgagcgcttcctgaaacgtatcaaggttccgtgccaccgtatcggtgacaaagaaatccacgtactgggtg
ataccaagagcgtcgtgctgtccatgccgattaacgagcagtttaagaaagctgtgctgaacgccgctaacgacgaaaa
ctacgcgctggtggcgtaataatactagagaaagaggagaaatactagatggtgagcaagggcgaggaggataacatgg
ccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcga
gggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgg
gacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagc
tgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactc
ctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcag
aagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcaga
ggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcc
cggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgc
gccgagggccgccactccaccggcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataaaacga
aaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggct
caccttcgggtgggcctttctgcgtttata