##gff-version 3
##sequence-region BBa_J58120 1 3821
BBa_J58120	.	engineered_region	913	2838	.	+	0	ID=BBa_J58119;Name=BBa_J58119
BBa_J58120	.	engineered_region	1909	2838	.	+	0	ID=BBa_P0451;Name=BBa_P0451;Parent=BBa_J58119
BBa_J58120	.	CDS	1927	2676	.	+	0	ID=BBa_C0051;Name=BBa_C0051;Parent=BBa_P0451
BBa_J58120	.	engineered_tag	2677	2701	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_J58120	.	tag	2638	2670	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_J58120	.	CDS	1930	2637	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_J58120	.	terminator	2798	2838	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0451
BBa_J58120	.	polyA_site	2825	2838	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J58120	.	engineered_region	2798	2838	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J58120	.	stem_loop	2805	2824	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J58120	.	stop_codon	2831	2831	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J58120	.	terminator	2710	2789	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0451
BBa_J58120	.	engineered_region	2710	2789	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J58120	.	stem_loop	2721	2764	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J58120	.	ribosome_entry_site	1909	1920	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_P0451
BBa_J58120	.	conserved_region	1913	1916	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J58120	.	engineered_region	913	1900	.	+	0	ID=BBa_J58117;Name=BBa_J58117;Parent=BBa_J58119
BBa_J58120	.	engineered_region	999	1900	.	+	0	ID=BBa_Q04400;Name=BBa_Q04400;Parent=BBa_J58117
BBa_J58120	.	ribosome_entry_site	999	1010	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_Q04400
BBa_J58120	.	conserved_region	1003	1006	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J58120	.	terminator	1710	1789	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_Q04400
BBa_J58120	.	engineered_region	1710	1789	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J58120	.	stem_loop	1721	1764	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J58120	.	promoter	1847	1900	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_Q04400
BBa_J58120	.	engineered_region	1847	1900	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J58120	.	promoter	1866	1871	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J58120	.	promoter	1889	1894	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J58120	.	non_covalent_binding_site	1847	1865	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J58120	.	non_covalent_binding_site	1872	1890	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J58120	.	terminator	1798	1838	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_Q04400
BBa_J58120	.	polyA_site	1825	1838	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J58120	.	engineered_region	1798	1838	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J58120	.	stem_loop	1805	1824	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J58120	.	stop_codon	1831	1831	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J58120	.	CDS	1017	1676	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_Q04400
BBa_J58120	.	CDS	1020	1636	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_J58120	.	tag	1637	1670	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_J58120	.	engineered_tag	1677	1701	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_J58120	.	promoter	913	990	.	+	0	ID=BBa_R0083;Name=BBa_R0083;Parent=BBa_J58117
BBa_J58120	.	sequence_feature	949	956	.	+	0	ID=annotation301223;Name=linker;Parent=BBa_R0083
BBa_J58120	.	non_covalent_binding_site	925	942	.	+	0	ID=annotation301221;Name=C1 OmpR;Parent=BBa_R0083
BBa_J58120	.	promoter	957	962	.	+	0	ID=annotation301224;Name=-35;Parent=BBa_R0083
BBa_J58120	.	promoter	980	985	.	+	0	ID=annotation301226;Name=-10;Parent=BBa_R0083
BBa_J58120	.	point_mutation	953	953	.	+	0	ID=annotation306881;Name=A;Parent=BBa_R0083
BBa_J58120	.	engineered_region	2847	3821	.	+	0	ID=BBa_J58101;Name=BBa_J58101
BBa_J58120	.	promoter	2847	2952	.	+	0	ID=BBa_J58100;Name=BBa_J58100;Parent=BBa_J58101
BBa_J58120	.	sequence_feature	2924	2940	.	+	0	ID=annotation1908181;Name=spacer from I12007 (54-70);Parent=BBa_J58100
BBa_J58120	.	operator	2849	2870	.	+	0	ID=annotation1908168;Name=CRP binding site;Parent=BBa_J58100
BBa_J58120	.	promoter	2941	2946	.	+	0	ID=annotation1908179;Name=-10;Parent=BBa_J58100
BBa_J58120	.	operator	2903	2920	.	+	0	ID=annotation1908169;Name=lam-cI OR2 binding site;Parent=BBa_J58100
BBa_J58120	.	sequence_feature	2947	2952	.	+	0	ID=annotation1908182;Name=spacer from I12007 (77-82);Parent=BBa_J58100
BBa_J58120	.	promoter	2918	2923	.	+	0	ID=annotation1908178;Name=-35;Parent=BBa_J58100
BBa_J58120	.	sequence_feature	2896	2902	.	+	0	ID=annotation1908180;Name=spacer from I12007 (26-32);Parent=BBa_J58100
BBa_J58120	.	sequence_feature	2871	2895	.	+	0	ID=annotation1908171;Name=spacer from I12007 (1-11, 59-72);Parent=BBa_J58100
BBa_J58120	.	engineered_region	2961	3821	.	+	0	ID=BBa_I13507;Name=BBa_I13507;Parent=BBa_J58101
BBa_J58120	.	CDS	2979	3659	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_I13507
BBa_J58120	.	CDS	2979	3653	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_J58120	.	engineered_tag	3660	3684	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_J58120	.	ribosome_entry_site	2961	2972	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13507
BBa_J58120	.	conserved_region	2965	2968	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J58120	.	terminator	3781	3821	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13507
BBa_J58120	.	polyA_site	3808	3821	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J58120	.	engineered_region	3781	3821	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J58120	.	stem_loop	3788	3807	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J58120	.	stop_codon	3814	3814	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J58120	.	terminator	3693	3772	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13507
BBa_J58120	.	engineered_region	3693	3772	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J58120	.	stem_loop	3704	3747	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J58120	.	engineered_region	1	904	.	+	0	ID=BBa_J58116;Name=BBa_J58116
BBa_J58120	.	ribosome_entry_site	117	128	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J58116
BBa_J58120	.	conserved_region	121	124	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J58120	.	terminator	864	904	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J58116
BBa_J58120	.	polyA_site	891	904	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J58120	.	engineered_region	864	904	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J58120	.	stem_loop	871	890	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J58120	.	stop_codon	897	897	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J58120	.	terminator	776	855	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J58116
BBa_J58120	.	engineered_region	776	855	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J58120	.	stem_loop	787	830	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J58120	.	promoter	1	108	.	+	0	ID=BBa_R0082;Name=BBa_R0082;Parent=BBa_J58116
BBa_J58120	.	promoter	75	80	.	+	0	ID=annotation301166;Name=-35;Parent=BBa_R0082
BBa_J58120	.	non_covalent_binding_site	54	71	.	+	0	ID=annotation301156;Name=C3 OmpR;Parent=BBa_R0082
BBa_J58120	.	non_covalent_binding_site	13	30	.	+	0	ID=annotation301154;Name=C1 OmpR;Parent=BBa_R0082
BBa_J58120	.	promoter	98	103	.	+	0	ID=annotation301167;Name=-10;Parent=BBa_R0082
BBa_J58120	.	non_covalent_binding_site	34	51	.	+	0	ID=annotation301155;Name=C2 OmpR;Parent=BBa_R0082
BBa_J58120	.	CDS	135	767	.	+	0	ID=BBa_J58112;Name=BBa_J58112;Parent=BBa_J58116
>BBa_J58120
tcccttgcatttacattttgaaacatctatagcgataaatgaaacatcttaaaagttttagtatcatattcgtgttgga
ttattctgcatttttggggagaatggacttactagagaaagaggagaaatactagatggtgcttggcaaaccgcaaaca
gacccgactctcgaatggttcttgtctcattgccacattcataagtacccatccaagagcacgcttattcaccagggtg
aaaaagcggaaacgctgtactacatcgttaaaggctctgtggcagtgctgatcaaagacgaagagggtaaagaaatgat
cctctcctatctgaatcagggtgattttattggcgaactgggcctgtttgaagagggccaggaacgtagcgcatgggta
cgtgcgaaaaccgcctgtgaagtggctgaaatttcgtacaaaaaatttcgccaattgattcaggtaaacccggacattc
tgatgcgtttgtctgcacagatggcgcgtcgtctgcaagtcacttcagagaaagtgggcaacctggcgttcctcgacgt
gacgggccgcattgcacagactctgctgaatctggcaaaacaaccagacgctatgactcacccggacggtatgcaaatc
aaaattacccgtcaggaaattggtcagattgtcggctgttctcgtgaaaccgtgggacgcattctgaagatgctggaag
atcagaacctgatctccgcacacggtaaaaccatcgtcgtttacggcactcgttaatactagagccaggcatcaaataa
aacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacac
tggctcaccttcgggtgggcctttctgcgtttatatactagagtcccttgcatttacattttgaaacatctatagcgat
ctcttgagttggattattctgcatttttggggagaatggacttactagagaaagaggagaaatactagatgtccagatt
agataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactc
gcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagcca
ttgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgc
taaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacag
tatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcg
ctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctac
tactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattc
ggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacg
ctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcag
tcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgg
gtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactga
gcactactagagaaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcac
gtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggat
ggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaatt
ctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagc
cgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctt
taccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggt
aattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctg
ttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtca
ggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatc
gctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagt
gctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatc
tgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactag
agagaaatgtgatctagatcacatttgcaaccattataaatagtggtgataaaatagtcaacacgcacggtgttagata
tttataaatagtggtgatagatttaacgttactagagaaagaggagaaatactagatggcttcctccgaagacgttatc
aaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaag
gtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcct
gtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttc
ccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgc
aagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaac
catgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaa
ctgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgctt
acaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaagg
tcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaa
aggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctc
accttcgggtgggcctttctgcgtttata