##gff-version 3
##sequence-region BBa_J58119 1 1926
BBa_J58119	.	engineered_region	997	1926	.	+	0	ID=BBa_P0451;Name=BBa_P0451
BBa_J58119	.	terminator	1798	1877	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0451
BBa_J58119	.	engineered_region	1798	1877	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J58119	.	stem_loop	1809	1852	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J58119	.	ribosome_entry_site	997	1008	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_P0451
BBa_J58119	.	conserved_region	1001	1004	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J58119	.	CDS	1015	1764	.	+	0	ID=BBa_C0051;Name=BBa_C0051;Parent=BBa_P0451
BBa_J58119	.	tag	1726	1758	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_J58119	.	CDS	1018	1725	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_J58119	.	engineered_tag	1765	1789	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_J58119	.	terminator	1886	1926	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0451
BBa_J58119	.	engineered_region	1886	1926	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J58119	.	polyA_site	1913	1926	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J58119	.	stop_codon	1919	1919	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J58119	.	stem_loop	1893	1912	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J58119	.	engineered_region	1	988	.	+	0	ID=BBa_J58117;Name=BBa_J58117
BBa_J58119	.	engineered_region	87	988	.	+	0	ID=BBa_Q04400;Name=BBa_Q04400;Parent=BBa_J58117
BBa_J58119	.	promoter	935	988	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_Q04400
BBa_J58119	.	non_covalent_binding_site	960	978	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J58119	.	engineered_region	935	988	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J58119	.	promoter	954	959	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J58119	.	non_covalent_binding_site	935	953	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J58119	.	promoter	977	982	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J58119	.	CDS	105	764	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_Q04400
BBa_J58119	.	tag	725	758	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_J58119	.	engineered_tag	765	789	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_J58119	.	CDS	108	724	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_J58119	.	ribosome_entry_site	87	98	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_Q04400
BBa_J58119	.	conserved_region	91	94	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J58119	.	terminator	886	926	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_Q04400
BBa_J58119	.	engineered_region	886	926	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J58119	.	polyA_site	913	926	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J58119	.	stop_codon	919	919	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J58119	.	stem_loop	893	912	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J58119	.	terminator	798	877	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_Q04400
BBa_J58119	.	engineered_region	798	877	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J58119	.	stem_loop	809	852	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J58119	.	promoter	1	78	.	+	0	ID=BBa_R0083;Name=BBa_R0083;Parent=BBa_J58117
BBa_J58119	.	sequence_feature	37	44	.	+	0	ID=annotation301223;Name=linker;Parent=BBa_R0083
BBa_J58119	.	point_mutation	41	41	.	+	0	ID=annotation306881;Name=A;Parent=BBa_R0083
BBa_J58119	.	promoter	68	73	.	+	0	ID=annotation301226;Name=-10;Parent=BBa_R0083
BBa_J58119	.	non_covalent_binding_site	13	30	.	+	0	ID=annotation301221;Name=C1 OmpR;Parent=BBa_R0083
BBa_J58119	.	promoter	45	50	.	+	0	ID=annotation301224;Name=-35;Parent=BBa_R0083
>BBa_J58119
tcccttgcatttacattttgaaacatctatagcgatctcttgagttggattattctgcatttttggggagaatggactt
actagagaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaat
gaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatg
taaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttaga
aggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagca
aaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaag
gtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaaga
gcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaatta
tttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaat
gtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcact
actagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtga
acgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgat
agagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgagcacaaaaa
agaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttgg
cttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgca
ttaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagag
aaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgt
tcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaa
gccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctg
acggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatga
gtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatccca
tgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacg
aaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacga
aaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggct
caccttcgggtgggcctttctgcgtttata