##gff-version 3
##sequence-region BBa_J54153 1 2182
BBa_J54153	.	ribosome_entry_site	1305	1319	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_J54153	.	ribosome_entry_site	1305	1319	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_J54153	.	engineered_region	1305	1319	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_J54153	.	ribosome_entry_site	1312	1316	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_J54153	.	terminator	2054	2133	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J54153	.	stem_loop	2065	2108	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J54153	.	engineered_region	2054	2133	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J54153	.	promoter	1	1210	.	+	0	ID=BBa_I0500;Name=BBa_I0500
BBa_J54153	.	sequence_feature	1	1079	.	+	0	ID=annotation1498843;Name=AraC;Parent=BBa_I0500
BBa_J54153	.	promoter	1080	1210	.	+	0	ID=annotation1498841;Name=Pbad;Parent=BBa_I0500
BBa_J54153	.	promoter	1043	1114	.	+	0	ID=annotation1498842;Name=PC;Parent=BBa_I0500
BBa_J54153	.	tag	1219	1237	.	+	0	ID=BBa_J54033;Name=BBa_J54033
BBa_J54153	.	tag	1233	1237	.	+	0	ID=annotation1903324;Name=BamHI;Parent=BBa_J54033
BBa_J54153	.	tag	1219	1223	.	+	0	ID=annotation1903323;Name=SalI;Parent=BBa_J54033
BBa_J54153	.	tag	1292	1296	.	+	0	ID=BBa_J54034;Name=BBa_J54034
BBa_J54153	.	tag	1292	1296	.	+	0	ID=annotation1903325;Name=MulI;Parent=BBa_J54034
BBa_J54153	.	terminator	2142	2182	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J54153	.	stem_loop	2149	2168	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J54153	.	stop_codon	2175	2175	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J54153	.	polyA_site	2169	2182	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J54153	.	engineered_region	2142	2182	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J54153	.	promoter	1246	1283	.	+	0	ID=BBa_J54230;Name=BBa_J54230
BBa_J54153	.	tag	1271	1277	.	+	0	ID=annotation1904013;Name=BglII;Parent=BBa_J54230
BBa_J54153	.	non_covalent_binding_site	1246	1270	.	+	0	ID=annotation1904014;Name=TetR;Parent=BBa_J54230
BBa_J54153	.	CDS	1326	2045	.	+	0	ID=BBa_E0040;Name=BBa_E0040
BBa_J54153	.	CDS	1326	2045	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
>BBa_J54153
ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcat
tttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtgg
tgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaa
aagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtga
tcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgcc
gcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttg
cccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccag
ttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgac
cgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcac
caccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataa
ccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcg
cttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactg
cgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaa
gccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacac
tttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttc
tccatacccgtttttttgggctagctactagaggtcgactgacgactggatctactagaggatcgtccctatcagtgat
agagatagatctatcgtaatactagagcgcgttactagagattaaagaggagaaatactagatgcgtaaaggagaagaa
cttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagg
gtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaac
acttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaag
agtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaag
tcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttgg
acacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaac
ttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatg
gccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagacca
catggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagc
caggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
tactagagtcacactggctcaccttcgggtgggcctttctgcgtttata