##gff-version 3
##sequence-region BBa_J54150 1 2150
BBa_J54150	.	terminator	2022	2101	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J54150	.	stem_loop	2033	2076	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J54150	.	engineered_region	2022	2101	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J54150	.	terminator	2110	2150	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J54150	.	stem_loop	2117	2136	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J54150	.	stop_codon	2143	2143	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J54150	.	polyA_site	2137	2150	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J54150	.	engineered_region	2110	2150	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J54150	.	ribosome_entry_site	1273	1287	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_J54150	.	ribosome_entry_site	1273	1287	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_J54150	.	engineered_region	1273	1287	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_J54150	.	ribosome_entry_site	1280	1284	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_J54150	.	tag	1246	1264	.	+	0	ID=BBa_J54025;Name=BBa_J54025
BBa_J54150	.	tag	1259	1264	.	+	0	ID=annotation1903322;Name=MulI;Parent=BBa_J54025
BBa_J54150	.	tag	1246	1250	.	+	0	ID=annotation1903321;Name=BglII;Parent=BBa_J54025
BBa_J54150	.	CDS	1294	2013	.	+	0	ID=BBa_E0040;Name=BBa_E0040
BBa_J54150	.	CDS	1294	2013	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_J54150	.	promoter	1	1210	.	+	0	ID=BBa_I0500;Name=BBa_I0500
BBa_J54150	.	sequence_feature	1	1079	.	+	0	ID=annotation1498843;Name=AraC;Parent=BBa_I0500
BBa_J54150	.	promoter	1080	1210	.	+	0	ID=annotation1498841;Name=Pbad;Parent=BBa_I0500
BBa_J54150	.	promoter	1043	1114	.	+	0	ID=annotation1498842;Name=PC;Parent=BBa_I0500
BBa_J54150	.	tag	1219	1237	.	+	0	ID=BBa_J54033;Name=BBa_J54033
BBa_J54150	.	tag	1233	1237	.	+	0	ID=annotation1903324;Name=BamHI;Parent=BBa_J54033
BBa_J54150	.	tag	1219	1223	.	+	0	ID=annotation1903323;Name=SalI;Parent=BBa_J54033
>BBa_J54150
ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcat
tttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtgg
tgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaa
aagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtga
tcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgcc
gcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttg
cccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccag
ttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgac
cgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcac
caccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataa
ccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcg
cttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactg
cgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaa
gccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacac
tttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttc
tccatacccgtttttttgggctagctactagaggtcgactgacgactggatctactagaggatctgtcggataacgcgt
tactagagattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttga
attagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacc
cttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaat
gctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaag
aactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaataga
atcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacaca
atgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaag
cgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctg
tccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctg
ggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcga
aagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgg
gcctttctgcgtttata