##gff-version 3
##sequence-region BBa_J54111 1 1002
BBa_J54111	.	stem_loop	969	988	.	+	0	ID=annotation1903829;Name=T7 TE
BBa_J54111	.	tag	111	116	.	+	0	ID=annotation1903820;Name=MulI
BBa_J54111	.	stop_codon	995	995	.	+	0	ID=annotation1903831;Name=stop
BBa_J54111	.	engineered_region	125	139	.	+	0	ID=annotation1903823;Name=BBa_B0030
BBa_J54111	.	engineered_region	98	116	.	+	0	ID=annotation1903821;Name=BBa_J54025
BBa_J54111	.	stem_loop	885	928	.	+	0	ID=annotation1903827;Name=stem_loop
BBa_J54111	.	tag	98	102	.	+	0	ID=annotation1903819;Name=BglII
BBa_J54111	.	tag	14	18	.	+	0	ID=annotation1903816;Name=BamHI
BBa_J54111	.	engineered_region	1	5	.	+	0	ID=annotation1903815;Name=BBa_J54023
BBa_J54111	.	operator	19	87	.	+	0	ID=annotation1903817;Name=MelR_regulated promoter
BBa_J54111	.	engineered_region	146	865	.	+	0	ID=annotation1903825;Name=BBa_E0040
BBa_J54111	.	engineered_region	962	1002	.	+	0	ID=annotation1903828;Name=BBa_B0012
BBa_J54111	.	engineered_region	14	89	.	+	0	ID=annotation1903818;Name=BBa_J54110
BBa_J54111	.	tag	1	5	.	+	0	ID=annotation1903814;Name=Sal
BBa_J54111	.	polyA_site	989	1002	.	+	0	ID=annotation1903830;Name=polya
BBa_J54111	.	ribosome_entry_site	132	136	.	+	0	ID=annotation1903824;Name=RBS
BBa_J54111	.	ribosome_entry_site	125	139	.	+	0	ID=annotation1903822;Name=RBS-1\Strong
BBa_J54111	.	engineered_region	874	953	.	+	0	ID=annotation1903826;Name=BBa_B0010
>BBa_J54111
gtcgacatgcgcggatccatctgagtttatggaatgccattacctgaaaagcagaggttttctgcggattcccctgcca
tgttgaagttgatcctgtcggagatctgtcggaaaacgcgtactagagattaaagaggagaaatactagatgcgtaaag
gagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcag
tggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttcca
tggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgact
ttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacg
tgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaac
attcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatca
aagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaat
tggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaag
agagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataat
actagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtga
acgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata