##gff-version 3
##sequence-region BBa_J47863 1 1126
BBa_J47863	.	engineered_region	228	989	.	+	0	ID=BBa_E0022;Name=BBa_E0022
BBa_J47863	.	stop_codon	984	989	.	+	0	ID=annotation2154;Name=2;Parent=BBa_E0022
BBa_J47863	.	point_mutation	296	296	.	+	0	ID=annotation2155;Name=A;Parent=BBa_E0022
BBa_J47863	.	tag	946	983	.	+	0	ID=annotation2153;Name=SsrA;Parent=BBa_E0022
BBa_J47863	.	CDS	228	989	.	+	0	ID=annotation2150;Name=CFP (LVA);Parent=BBa_E0022
BBa_J47863	.	engineered_region	228	989	.	+	0	ID=annotation7040;Name=BBa_E0022;Parent=BBa_E0022
BBa_J47863	.	terminator	998	1077	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J47863	.	stem_loop	1009	1052	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J47863	.	engineered_region	998	1077	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J47863	.	terminator	1086	1126	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J47863	.	stop_codon	1119	1119	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J47863	.	stem_loop	1093	1112	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J47863	.	polyA_site	1113	1126	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J47863	.	engineered_region	1086	1126	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J47863	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_J47863	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_J47863	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_J47863	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_J47863	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_J47863	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_J47863	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_J47863	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_J47863	.	ribosome_entry_site	209	221	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_J47863	.	engineered_region	209	221	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_J47863	.	ribosome_entry_site	209	221	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_J47863	.	ribosome_entry_site	215	218	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
>BBa_J47863
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagtcacacaggaaagtactagatggtgagca
agggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgt
gtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtg
ccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacg
acttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagac
ccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggc
aacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggca
tcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccc
catcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgag
aagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggc
ctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagt
cgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcggg
tgggcctttctgcgtttata