##gff-version 3
##sequence-region BBa_J47862 1 1127
BBa_J47862	.	engineered_region	229	990	.	+	0	ID=BBa_E0022;Name=BBa_E0022
BBa_J47862	.	point_mutation	297	297	.	+	0	ID=annotation2155;Name=A;Parent=BBa_E0022
BBa_J47862	.	CDS	229	990	.	+	0	ID=annotation2150;Name=CFP (LVA);Parent=BBa_E0022
BBa_J47862	.	stop_codon	985	990	.	+	0	ID=annotation2154;Name=2;Parent=BBa_E0022
BBa_J47862	.	tag	947	984	.	+	0	ID=annotation2153;Name=SsrA;Parent=BBa_E0022
BBa_J47862	.	engineered_region	229	990	.	+	0	ID=annotation7040;Name=BBa_E0022;Parent=BBa_E0022
BBa_J47862	.	ribosome_entry_site	209	222	.	+	0	ID=BBa_B0031;Name=BBa_B0031
BBa_J47862	.	conserved_region	215	218	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
BBa_J47862	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_J47862	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_J47862	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_J47862	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_J47862	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_J47862	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_J47862	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_J47862	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_J47862	.	terminator	1087	1127	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J47862	.	engineered_region	1087	1127	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J47862	.	stem_loop	1094	1113	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J47862	.	polyA_site	1114	1127	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J47862	.	stop_codon	1120	1120	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J47862	.	terminator	999	1078	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J47862	.	engineered_region	999	1078	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J47862	.	stem_loop	1010	1053	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
>BBa_J47862
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagtcacacaggaaacctactagatggtgagc
aagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcg
tgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgt
gccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcac
gacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaaga
cccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacgg
caacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggc
atcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacaccc
ccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacga
gaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagagg
cctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcag
tcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgg
gtgggcctttctgcgtttata