##gff-version 3
##sequence-region BBa_J45900 1 4886
BBa_J45900	.	terminator	4758	4837	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J45900	.	stem_loop	4769	4812	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J45900	.	engineered_region	4758	4837	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J45900	.	ribosome_entry_site	64	78	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_J45900	.	engineered_region	64	78	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_J45900	.	ribosome_entry_site	71	75	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_J45900	.	ribosome_entry_site	64	78	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_J45900	.	CDS	1248	3077	.	+	0	ID=BBa_J45009;Name=BBa_J45009
BBa_J45900	.	start_codon	1248	1250	.	+	0	ID=annotation1961303;Name=start codon;Parent=BBa_J45009
BBa_J45900	.	point_mutation	1253	1253	.	+	0	ID=annotation1891628;Name=t->c to eliminate EcoRI, to do;Parent=BBa_J45009
BBa_J45900	.	point_mutation	2153	2153	.	+	0	ID=annotation1891629;Name=a->g to eliminate EcoRI, to do;Parent=BBa_J45009
BBa_J45900	.	point_mutation	3038	3038	.	+	0	ID=annotation1891631;Name=a->g to eliminate EcoRI, to do;Parent=BBa_J45009
BBa_J45900	.	CDS	1248	3077	.	+	0	ID=annotation1961298;Name=THI3;Parent=BBa_J45009
BBa_J45900	.	point_mutation	2852	2852	.	+	0	ID=annotation1891630;Name=a->g to eliminate EcoRI, to do;Parent=BBa_J45009
BBa_J45900	.	stop_codon	3075	3077	.	+	0	ID=annotation1961304;Name=stop codon;Parent=BBa_J45009
BBa_J45900	.	point_mutation	1946	1946	.	+	0	ID=annotation1891632;Name=t->c to eliminate SpeI, to do;Parent=BBa_J45009
BBa_J45900	.	CDS	85	1218	.	+	0	ID=BBa_J45008;Name=BBa_J45008
BBa_J45900	.	point_mutation	855	855	.	+	0	ID=annotation1894553;Name=a->g to eliminate SpeI;Parent=BBa_J45008
BBa_J45900	.	start_codon	85	87	.	+	0	ID=annotation1883624;Name=start codon;Parent=BBa_J45008
BBa_J45900	.	stop_codon	1213	1218	.	+	0	ID=annotation1883625;Name=double TAA stop codon;Parent=BBa_J45008
BBa_J45900	.	CDS	85	1218	.	+	0	ID=annotation1961299;Name=BAT2;Parent=BBa_J45008
BBa_J45900	.	CDS	3169	4749	.	+	0	ID=BBa_J45014;Name=BBa_J45014
BBa_J45900	.	CDS	3169	4749	.	+	0	ID=annotation1961300;Name=ATF1;Parent=BBa_J45014
BBa_J45900	.	stop_codon	4744	4749	.	+	0	ID=annotation1961302;Name=double TAA stop codon;Parent=BBa_J45014
BBa_J45900	.	sequence_alteration	4316	4316	.	+	0	ID=annotation1961301;Name=t -> c;Parent=BBa_J45014
BBa_J45900	.	point_mutation	3582	3582	.	+	0	ID=annotation1961305;Name=a->g to eliminate EcoRI site;Parent=BBa_J45014
BBa_J45900	.	promoter	1	54	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_J45900	.	operator	26	42	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_J45900	.	promoter	20	25	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_J45900	.	promoter	43	48	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_J45900	.	operator	3	19	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_J45900	.	engineered_region	1	54	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_J45900	.	ribosome_entry_site	3148	3162	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_J45900	.	engineered_region	3148	3162	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_J45900	.	ribosome_entry_site	3155	3159	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_J45900	.	ribosome_entry_site	3148	3162	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_J45900	.	promoter	3086	3139	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_J45900	.	engineered_region	3086	3139	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J45900	.	promoter	3105	3110	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J45900	.	non_covalent_binding_site	3111	3129	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J45900	.	promoter	3128	3133	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J45900	.	non_covalent_binding_site	3086	3104	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J45900	.	ribosome_entry_site	1227	1241	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_J45900	.	engineered_region	1227	1241	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_J45900	.	ribosome_entry_site	1234	1238	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_J45900	.	ribosome_entry_site	1227	1241	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_J45900	.	terminator	4846	4886	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J45900	.	stem_loop	4853	4872	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J45900	.	polyA_site	4873	4886	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J45900	.	stop_codon	4879	4879	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J45900	.	engineered_region	4846	4886	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
>BBa_J45900
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagattaaagaggagaaat
actagatgaccttggcacccctagacgcctccaaagttaagataactaccacacaacatgcatctaagccaaaaccgaa
cagtgagttagtgtttggcaagagcttcacggaccacatgttaactgcggaatggacagctgaaaaagggtggggtacc
ccagagattaaaccttatcaaaatctgtctttagacccttccgcggtggttttccattatgcttttgagctattcgaag
ggatgaaggcttacagaacggtggacaacaaaattacaatgtttcgtccagatatgaatatgaagcgcatgaataagtc
tgctcagagaatctgtttgccaacgttcgacccagaagagttgattaccctaattgggaaactgatccagcaagataag
tgcttagttcctgaaggaaaaggttactctttatatatcaggcctacattaatcggcactacggccggtttaggggttt
ccacgcctgatagagccttgctatatgtcatttgctgccctgtgggtccttattacaaaactggatttaaggcggtcag
actggaagccactgattatgccacaagagcttggccaggaggctgtggtgacaagaaactaggtgcaaactacgccccc
tgcgtcctgccacaattgcaagctgcttcaaggggttaccaacaaaatttatggctatttggtccaaataacaacatta
ctgaagtcggcaccatgaatgcttttttcgtgtttaaagatagtaaaacgggcaagaaggaactggttactgctccact
agacggtaccattttggaaggtgttactagggattccattttaaatcttgctaaagaaagactcgaaccaagtgaatgg
accattagtgaacgctacttcactataggcgaagttactgagagatccaagaacggtgaactacttgaagcctttggtt
ctggtactgctgcgattgtttctcccattaaggaaatcggctggaaaggcgaacaaattaatattccgttgttgcccgg
cgaacaaaccggtccattggccaaagaagttgcacaatggattaatggaatccaatatggcgagactgagcatggcaat
tggtcaagggttgttactgatttgaactaataatactagagattaaagaggagaaatactagatgaattctagctatac
acagagatatgcactgccgaagtgtatagcaatatcagattatcttttccatcggctcaaccagctgaacatacatacc
atatttggactctccggagaatttagcatgccgttgctggataaactatacaacattccgaacttacgatgggccggta
attctaatgagttaaatgctgcctacgcagcagatggatactcacgactaaaaggcttgggatgtctcataacaacctt
tggtgtaggcgaattatcggcaatcaatggcgtggccggatcttacgctgaacatgtaggaatacttcacatagtgggt
atgccgccaacaagtgcacaaacgaaacaactactactgcatcatactctgggcaatggtgatttcacggtatttcata
gaatagccagtgatgtagcatgctatacaacattgattattgactctgaattatgtgccgacgaagtcgataagtgcat
caaaaaggcttggatagaacagaggccagtatacatgggcatgcctgtcaaccaggtaaatctcccgattgaatcagca
aggcttaatacacctctggatttacaattgcataaaaacgacccagacgtagagaaagaagttatttctcgaatattga
gttttatatacaaaagccagaatccggcaatcatcgtagatgcatgtactagtcgacagaatttaatcgaggagactaa
agagctttgtaataggcttaaatttccagtttttgttacacctatgggtaagggtacagtaaacgaaacagacccgcaa
tttgggggcgtattcacgggctcgatatcagccccagaagtaagagaagtagttgattttgccgattttatcatcgtca
ttggttgcatgctctccgaattcagcacgtcaactttccacttccaatataaaactaagaattgtgcgctactatattc
tacatctgtgaaattgaaaaatgccacatatcctgacttgagcattaaattactactacagaaaatattagcaaatctt
gatgaatctaaactgtcttaccaaccaagcgaacaacccagtatgatggttccaagaccttacccagcaggaaatgtcc
tcttgagacaagaatgggtctggaatgaaatatcccattggttccaaccaggtgacataatcataacagaaactggtgc
ttctgcatttggagttaaccagaccagatttccggtaaatacactaggtatttcgcaagctctttggggatctgtcgga
tatacaatgggggcgtgtcttggggcagaatttgctgttcaagagataaacaaggataaattccccgcaactaaacata
gagttattctgtttatgggtgacggtgctttccaattgacagttcaagaattatccacaattgttaagtggggattgac
accttatatttttgtgatgaataaccaaggttactctgtggacaggtttttgcatcacaggtcagatgctagttattac
gatatccaaccttggaactacttgggattattgcgagtatttggttgcacgaactacgaaacgaaaaaaattattactg
ttggagaattcagatccatgatcagtgacccaaactttgcgaccaatgacaaaattcggatgatagagattatgctacc
accaagggatgttccacaggctctgcttgacaggtgggtggtagaaaaagaacagagcaaacaagtgcaagaggagaac
gaaaattctagcgcagtaaatacgccaactccagaattccaaccacttctaaaaaaaaatcaagttggatactgatact
agagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagattaaagaggaga
aatactagatgaatgaaatcgatgagaaaaatcaggcccccgtgcaacaagaatgcctgaaagagatgattcagaatgg
gcatgctcggcgtatgggatctgttgaagatctgtatgttgctctcaacagacaaaacttatatcgaaacttctgcaca
tatggagaattgagtgattactgtactagggatcagctcacattagctttgagggaaatctgcctgaaaaatccaactc
ttttacatattgttctaccaacaagatggccaaatcatgaaaattattatcgcagttccgaatactattcacggccaca
tccagtgcatgattatatttcagtattacaagaattgaaactgagtggtgtggttctcaatgaacaacctgagtacagt
gcagtaatgaagcaaatattagaagagttcaaaaatagtaagggttcctatactgcaaaaatttttaaacttactacca
ctttgactattccttactttggaccaacaggaccgagttggcggctaatttgtcttccagaagagcacacagaaaagtg
gaaaaaatttatctttgtatctaatcattgcatgtctgatggtcggtcttcgatccacttttttcatgatttaagagac
gaattaaataatattaaaactccaccaaaaaaattagattacattttcaagtacgaggaggattaccaattattgagga
aacttccagaaccgatcgaaaaggtgatagactttagaccaccgtacttgtttattccgaagtcacttctttcgggttt
catctacaatcatttgagattttcttcaaaaggtgtctgtatgagaatggatgatgtggaaaaaaccgatgatgttgtc
accgagatcatcaatatttcaccaacagaatttcaagcgattaaagcaaatattaaatcaaatatccaaggtaagtgta
ctatcactccgtttttacatgtttgttggtttgtatctcttcataaatggggtaaatttttcaaaccattgaacttcga
atggcttacggatatttttatccccgcagattgccgctcacaactaccagatgatgatgaaatgagacagatgtacaga
tatggcgctaacgttggatttattgacttcaccccctggataagcgaatctgacatgaatgataacaaagaaaattttt
ggccacttattgagcactaccatgaagtaatttcggaagctttaagaaataaaaagcatctccatggcttagggttcaa
tatacaaggcttcgttcaaaaatatgtgaacattgacaaggtaatgtgcgatcgtgccatcgggaaaagacgcggaggt
acattgttaagcaatgtaggtctgtttaatcagttagaggagcccgatgccaaatattctatatgcgatttggcatttg
gccaatttcaaggatcctggcaccaagcattttccttgggtgtttgttcgactaatgtaaaggggatgaatattgttgt
tgcttcaacaaagaatgttgttggtagtcaagaatctctcgaagagctttgctccatttacaaagctctccttttaggc
ccttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgt
ttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata