##gff-version 3 ##sequence-region BBa_J45219 1 1737 BBa_J45219 . ribosome_entry_site 1 13 . + 0 ID=BBa_B0032;Name=BBa_B0032 BBa_J45219 . engineered_region 1 13 . + 0 ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032 BBa_J45219 . ribosome_entry_site 7 10 . + 0 ID=annotation1710;Name=RBS;Parent=BBa_B0032 BBa_J45219 . ribosome_entry_site 1 13 . + 0 ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032 BBa_J45219 . terminator 1697 1737 . + 0 ID=BBa_B0012;Name=BBa_B0012 BBa_J45219 . polyA_site 1724 1737 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_J45219 . engineered_region 1697 1737 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_J45219 . stop_codon 1730 1730 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_J45219 . stem_loop 1704 1723 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_J45219 . CDS 20 1600 . + 0 ID=BBa_J45014;Name=BBa_J45014 BBa_J45219 . CDS 20 1600 . + 0 ID=annotation1961300;Name=ATF1;Parent=BBa_J45014 BBa_J45219 . sequence_alteration 1167 1167 . + 0 ID=annotation1961301;Name=t -> c;Parent=BBa_J45014 BBa_J45219 . stop_codon 1595 1600 . + 0 ID=annotation1961302;Name=double TAA stop codon;Parent=BBa_J45014 BBa_J45219 . point_mutation 433 433 . + 0 ID=annotation1961305;Name=a->g to eliminate EcoRI site;Parent=BBa_J45014 BBa_J45219 . terminator 1609 1688 . + 0 ID=BBa_B0010;Name=BBa_B0010 BBa_J45219 . engineered_region 1609 1688 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 BBa_J45219 . stem_loop 1620 1663 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 >BBa_J45219 tcacacaggaaagtactagatgaatgaaatcgatgagaaaaatcaggcccccgtgcaacaagaatgcctgaaagagatg attcagaatgggcatgctcggcgtatgggatctgttgaagatctgtatgttgctctcaacagacaaaacttatatcgaa acttctgcacatatggagaattgagtgattactgtactagggatcagctcacattagctttgagggaaatctgcctgaa aaatccaactcttttacatattgttctaccaacaagatggccaaatcatgaaaattattatcgcagttccgaatactat tcacggccacatccagtgcatgattatatttcagtattacaagaattgaaactgagtggtgtggttctcaatgaacaac ctgagtacagtgcagtaatgaagcaaatattagaagagttcaaaaatagtaagggttcctatactgcaaaaatttttaa acttactaccactttgactattccttactttggaccaacaggaccgagttggcggctaatttgtcttccagaagagcac acagaaaagtggaaaaaatttatctttgtatctaatcattgcatgtctgatggtcggtcttcgatccacttttttcatg atttaagagacgaattaaataatattaaaactccaccaaaaaaattagattacattttcaagtacgaggaggattacca attattgaggaaacttccagaaccgatcgaaaaggtgatagactttagaccaccgtacttgtttattccgaagtcactt ctttcgggtttcatctacaatcatttgagattttcttcaaaaggtgtctgtatgagaatggatgatgtggaaaaaaccg atgatgttgtcaccgagatcatcaatatttcaccaacagaatttcaagcgattaaagcaaatattaaatcaaatatcca aggtaagtgtactatcactccgtttttacatgtttgttggtttgtatctcttcataaatggggtaaatttttcaaacca ttgaacttcgaatggcttacggatatttttatccccgcagattgccgctcacaactaccagatgatgatgaaatgagac agatgtacagatatggcgctaacgttggatttattgacttcaccccctggataagcgaatctgacatgaatgataacaa agaaaatttttggccacttattgagcactaccatgaagtaatttcggaagctttaagaaataaaaagcatctccatggc ttagggttcaatatacaaggcttcgttcaaaaatatgtgaacattgacaaggtaatgtgcgatcgtgccatcgggaaaa gacgcggaggtacattgttaagcaatgtaggtctgtttaatcagttagaggagcccgatgccaaatattctatatgcga tttggcatttggccaatttcaaggatcctggcaccaagcattttccttgggtgtttgttcgactaatgtaaaggggatg aatattgttgttgcttcaacaaagaatgttgttggtagtcaagaatctctcgaagagctttgctccatttacaaagctc tccttttaggcccttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtt ttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata