##gff-version 3
##sequence-region BBa_J45100 1 1294
BBa_J45100	.	CDS	84	1157	.	+	0	ID=BBa_J45004;Name=BBa_J45004
BBa_J45100	.	stop_codon	1155	1157	.	+	0	ID=annotation1880520;Name=stop codon;Parent=BBa_J45004
BBa_J45100	.	start_codon	84	86	.	+	0	ID=annotation1880519;Name=start codon;Parent=BBa_J45004
BBa_J45100	.	CDS	84	1157	.	+	0	ID=annotation1880517;Name=BMST1;Parent=BBa_J45004
BBa_J45100	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_J45100	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J45100	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J45100	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J45100	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J45100	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J45100	.	terminator	1166	1245	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J45100	.	stem_loop	1177	1220	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J45100	.	engineered_region	1166	1245	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J45100	.	ribosome_entry_site	63	77	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_J45100	.	ribosome_entry_site	63	77	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_J45100	.	engineered_region	63	77	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_J45100	.	ribosome_entry_site	70	74	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_J45100	.	terminator	1254	1294	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J45100	.	stop_codon	1287	1287	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J45100	.	polyA_site	1281	1294	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J45100	.	stem_loop	1261	1280	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J45100	.	engineered_region	1254	1294	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
>BBa_J45100
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagattaaagaggagaaata
ctagatggaagttgttgaagttcttcacatgaatggaggaaatggagacagtagctatgcaaacaattctttggttcag
caaaaggtgattctcatgacaaagccaataactgagcaagccatgattgatctctacagcagcctctttccagaaacct
tatgcattgcagatttgggttgttctttgggagctaacactttcttggtggtctcacagcttgttaaaatagtagaaaa
agaacgaaaaaagcatggttttaagtctccagagttttattttcacttcaatgatcttcctggcaatgattttaataca
ctttttcagtcactgggggcatttcaagaagatttgagaaagcatataggggaaagctttggtccatgttttttcagtg
gagtgcctggttcattttatactagacttttcccttccaaaagtttacattttgtttactcctcctacagtctcatgtg
gctatctcaggtgcctaatgggattgaaaataacaagggaaacatttacatggcaagaacaagccctctaagtgttatt
aaagcatactacaagcaatatgaaatagatttttcaaattttctcaagtaccgttcagaggaattgatgaaaggtggaa
agatggtgttaacactcctaggtagagaaagtgaggatcctactagcaaagaatgctgttacatttgggagcttctagc
catggccctcaataagttggttgaagagggattgataaaagaagagaaagtagatgcattcaatattcctcaatacaca
ccatcaccagcagaagtaaagtacatagttgagaaggaaggatcattcaccattaatcgcttggaaacatcaagagttc
attggaatgcttctaataatgagaagaatggtggttacaatgtgtcaaggtgcatgagagctgtggctgagcctttgct
tgtcagccactttgacaaggaattgatggatttagtgttccacaagtacgaagagattgtttctgattgcatgtccaaa
gagaatactgagtttataaatgtcatcatctccttgaccaaaataaattaatactagagccaggcatcaaataaaacga
aaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggct
caccttcgggtgggcctttctgcgtttata