##gff-version 3
##sequence-region BBa_J43000 1 2189
BBa_J43000	.	engineered_region	1312	2189	.	+	0	ID=BBa_E0430;Name=BBa_E0430
BBa_J43000	.	CDS	1330	2052	.	+	0	ID=BBa_E0030;Name=BBa_E0030;Parent=BBa_E0430
BBa_J43000	.	terminator	2149	2189	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0430
BBa_J43000	.	engineered_region	2149	2189	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J43000	.	stop_codon	2182	2182	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J43000	.	stem_loop	2156	2175	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J43000	.	polyA_site	2176	2189	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J43000	.	terminator	2061	2140	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0430
BBa_J43000	.	stem_loop	2072	2115	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J43000	.	engineered_region	2061	2140	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J43000	.	ribosome_entry_site	1312	1323	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_E0430
BBa_J43000	.	conserved_region	1316	1319	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J43000	.	promoter	1	393	.	+	0	ID=BBa_J45503;Name=BBa_J45503
BBa_J43000	.	engineered_region	402	1303	.	+	0	ID=BBa_Q04400;Name=BBa_Q04400
BBa_J43000	.	ribosome_entry_site	402	413	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_Q04400
BBa_J43000	.	conserved_region	406	409	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J43000	.	CDS	420	1079	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_Q04400
BBa_J43000	.	engineered_tag	1080	1104	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_J43000	.	tag	1040	1073	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_J43000	.	CDS	423	1039	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_J43000	.	terminator	1201	1241	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_Q04400
BBa_J43000	.	engineered_region	1201	1241	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J43000	.	stop_codon	1234	1234	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J43000	.	stem_loop	1208	1227	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J43000	.	polyA_site	1228	1241	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J43000	.	promoter	1250	1303	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_Q04400
BBa_J43000	.	promoter	1269	1274	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J43000	.	non_covalent_binding_site	1275	1293	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J43000	.	engineered_region	1250	1303	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J43000	.	non_covalent_binding_site	1250	1268	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J43000	.	promoter	1292	1297	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J43000	.	terminator	1113	1192	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_Q04400
BBa_J43000	.	stem_loop	1124	1167	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J43000	.	engineered_region	1113	1192	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
>BBa_J43000
cgccgctatggactggataaagagatggtaatggatttctttcgtgagaataattcctgttctacgttgcgctttttta
tggccggttatcgcctcgaaaattgatcaaacatacgtattatcttgctttaattaattacactaatgcttcttccctt
cgttttagcgccccgccgcagtatcatgatatcgataaccataataaatgtgtggtaaatggcgcatcgatcgcattat
tgattttgcgattgaggcaaaatatatgccaggtcttcgcaacggaataactataaatgactggagataacaccctcat
ccattctcacggcattaaccgtcgtgatttcatgaagctttgtgcagcattagccgccaccatggggttaagtagcata
ctagagaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatg
aggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgt
aaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaa
ggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaa
aagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaagg
tttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagag
catcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattat
ttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatg
tgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcacta
ctagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaa
cgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgata
gagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatggtgagcaaggg
cgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtcc
ggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccct
ggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgactt
cttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgc
gccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaaca
tcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaa
ggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatc
ggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagc
gcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataata
ctagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaa
cgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata