##gff-version 3
##sequence-region BBa_J40003 1 5443
BBa_J40003	.	engineered_region	2168	5443	.	+	0	ID=BBa_I5612;Name=BBa_I5612
BBa_J40003	.	promoter	2168	2221	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_I5612
BBa_J40003	.	engineered_region	2168	2221	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J40003	.	promoter	2210	2215	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J40003	.	non_covalent_binding_site	2193	2211	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J40003	.	promoter	2187	2192	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J40003	.	non_covalent_binding_site	2168	2186	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J40003	.	engineered_region	3225	4595	.	+	0	ID=BBa_Q04121;Name=BBa_Q04121;Parent=BBa_I5612
BBa_J40003	.	terminator	4404	4532	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_Q04121
BBa_J40003	.	terminator	4492	4532	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_J40003	.	stop_codon	4525	4525	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J40003	.	polyA_site	4519	4532	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J40003	.	stem_loop	4499	4518	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J40003	.	engineered_region	4492	4532	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J40003	.	terminator	4404	4483	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_J40003	.	stem_loop	4415	4458	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J40003	.	engineered_region	4404	4483	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J40003	.	promoter	4541	4594	.	+	0	ID=BBa_R0011;Name=BBa_R0011;Parent=BBa_Q04121
BBa_J40003	.	promoter	4560	4565	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_J40003	.	promoter	4583	4588	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_J40003	.	operator	4566	4582	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_J40003	.	engineered_region	4541	4594	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_J40003	.	operator	4543	4559	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_J40003	.	CDS	3243	4370	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_Q04121
BBa_J40003	.	engineered_region	3243	4370	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_J40003	.	CDS	3243	4370	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_J40003	.	engineered_tag	4371	4395	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_J40003	.	tag	4332	4370	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_J40003	.	ribosome_entry_site	3225	3236	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_Q04121
BBa_J40003	.	conserved_region	3229	3232	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J40003	.	engineered_region	2230	3216	.	+	0	ID=BBa_Q04510;Name=BBa_Q04510;Parent=BBa_I5612
BBa_J40003	.	ribosome_entry_site	2230	2241	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_Q04510
BBa_J40003	.	conserved_region	2234	2237	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J40003	.	CDS	2248	2997	.	+	0	ID=BBa_C0051;Name=BBa_C0051;Parent=BBa_Q04510
BBa_J40003	.	engineered_tag	2998	3022	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_J40003	.	CDS	2251	2958	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_J40003	.	tag	2959	2991	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_J40003	.	terminator	3119	3159	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_Q04510
BBa_J40003	.	stop_codon	3152	3152	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J40003	.	polyA_site	3146	3159	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J40003	.	stem_loop	3126	3145	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J40003	.	engineered_region	3119	3159	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J40003	.	terminator	3031	3110	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_Q04510
BBa_J40003	.	stem_loop	3042	3085	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J40003	.	engineered_region	3031	3110	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J40003	.	promoter	3168	3216	.	+	0	ID=BBa_R0051;Name=BBa_R0051;Parent=BBa_Q04510
BBa_J40003	.	engineered_region	3168	3216	.	+	0	ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051
BBa_J40003	.	non_covalent_binding_site	3192	3208	.	+	0	ID=annotation2024;Name=OR1;Parent=BBa_R0051
BBa_J40003	.	promoter	3182	3187	.	+	0	ID=annotation2023;Name=-35;Parent=BBa_R0051
BBa_J40003	.	promoter	3205	3210	.	+	0	ID=annotation2022;Name=-10;Parent=BBa_R0051
BBa_J40003	.	non_covalent_binding_site	3168	3184	.	+	0	ID=annotation2025;Name=OR2;Parent=BBa_R0051
BBa_J40003	.	engineered_region	4604	5443	.	+	0	ID=BBa_P0440;Name=BBa_P0440;Parent=BBa_I5612
BBa_J40003	.	ribosome_entry_site	4604	4615	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_P0440
BBa_J40003	.	conserved_region	4608	4611	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J40003	.	CDS	4622	5281	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_P0440
BBa_J40003	.	CDS	4625	5241	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_J40003	.	engineered_tag	5282	5306	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_J40003	.	tag	5242	5275	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_J40003	.	terminator	5315	5394	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0440
BBa_J40003	.	stem_loop	5326	5369	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J40003	.	engineered_region	5315	5394	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J40003	.	terminator	5403	5443	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0440
BBa_J40003	.	stop_codon	5436	5436	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J40003	.	polyA_site	5430	5443	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J40003	.	stem_loop	5410	5429	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J40003	.	engineered_region	5403	5443	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J40003	.	engineered_region	1	2159	.	+	0	ID=BBa_J40001;Name=BBa_J40001
BBa_J40003	.	engineered_region	1243	2159	.	+	0	ID=BBa_E0422;Name=BBa_E0422;Parent=BBa_J40001
BBa_J40003	.	engineered_region	1261	2022	.	+	0	ID=BBa_E0022;Name=BBa_E0022;Parent=BBa_E0422
BBa_J40003	.	point_mutation	1329	1329	.	+	0	ID=annotation2155;Name=A;Parent=BBa_E0022
BBa_J40003	.	tag	1979	2016	.	+	0	ID=annotation2153;Name=SsrA;Parent=BBa_E0022
BBa_J40003	.	CDS	1261	2022	.	+	0	ID=annotation2150;Name=CFP (LVA);Parent=BBa_E0022
BBa_J40003	.	engineered_region	1261	2022	.	+	0	ID=annotation7040;Name=BBa_E0022;Parent=BBa_E0022
BBa_J40003	.	stop_codon	2017	2022	.	+	0	ID=annotation2154;Name=2;Parent=BBa_E0022
BBa_J40003	.	terminator	2119	2159	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0422
BBa_J40003	.	stop_codon	2152	2152	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J40003	.	polyA_site	2146	2159	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J40003	.	stem_loop	2126	2145	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J40003	.	engineered_region	2119	2159	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J40003	.	ribosome_entry_site	1243	1254	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_E0422
BBa_J40003	.	conserved_region	1247	1250	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J40003	.	terminator	2031	2110	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0422
BBa_J40003	.	stem_loop	2042	2085	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J40003	.	engineered_region	2031	2110	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J40003	.	engineered_region	64	1234	.	+	0	ID=BBa_S0100;Name=BBa_S0100;Parent=BBa_J40001
BBa_J40003	.	ribosome_entry_site	64	75	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S0100
BBa_J40003	.	conserved_region	68	71	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J40003	.	CDS	82	1209	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_S0100
BBa_J40003	.	engineered_region	82	1209	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_J40003	.	CDS	82	1209	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_J40003	.	engineered_tag	1210	1234	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_J40003	.	tag	1171	1209	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_J40003	.	promoter	1	55	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_J40001
BBa_J40003	.	engineered_region	1	55	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_J40003	.	non_covalent_binding_site	1	20	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_J40003	.	start_codon	53	53	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_J40003	.	promoter	42	47	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_J40003	.	promoter	20	25	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
>BBa_J40003
acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatact
agatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgt
ggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattccc
aaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgc
cgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaag
cggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctg
gatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacac
ccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagca
aatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgc
aatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctga
atgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtc
cgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgtta
accaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcgg
tgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctcc
ccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaa
aactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagaaagaggagaaatactagatgg
tgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagtt
cagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctg
cccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagc
agcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaacta
caagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggag
gacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaaga
acggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaa
cacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagacccc
aacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtaca
agaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaagg
ctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcacc
ttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagat
actgagcactactagagaaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgagga
cgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatg
gggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaa
aaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtat
gcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttaga
acctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttg
aaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagca
ggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagc
ggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaag
ttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctg
atagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtt
ttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatat
actagagtaacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagaaagaggagaaatac
tagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcg
tggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcc
caaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcg
ccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaa
gcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgct
ggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagaca
cccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagc
aaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcg
caatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctg
aatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagt
ccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgtt
aaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcg
gtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctc
cccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacga
aaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaa
aggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctc
accttcgggtgggcctttctgcgtttatatactagagaattgtgagcggataacaattgacattgtgagcggataacaa
gatactgagcacatactagagaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcat
tagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctac
attgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcac
ttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtc
atcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctt
tttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgta
ttggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgac
aagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattaga
aaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgc
tagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctg
ttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata