##gff-version 3
##sequence-region BBa_J331028 1 7029
BBa_J331028	.	ribosome_entry_site	4748	4759	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J331028	.	conserved_region	4752	4755	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J331028	.	terminator	6991	7029	.	+	0	ID=BBa_B1006;Name=BBa_B1006
BBa_J331028	.	polyA_site	7022	7029	.	+	0	ID=annotation1898430;Name=PolyA;Parent=BBa_B1006
BBa_J331028	.	polyA_site	6991	6999	.	+	0	ID=annotation1898431;Name=PolyA;Parent=BBa_B1006
BBa_J331028	.	engineered_region	6991	7029	.	+	0	ID=annotation1898428;Name=B1006;Parent=BBa_B1006
BBa_J331028	.	stem_loop	7000	7021	.	+	0	ID=annotation1898429;Name=modified thr terminator;Parent=BBa_B1006
BBa_J331028	.	ribosome_entry_site	2613	2624	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J331028	.	conserved_region	2617	2620	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J331028	.	ribosome_entry_site	209	220	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J331028	.	conserved_region	213	216	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J331028	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_J331028	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_J331028	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_J331028	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_J331028	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_J331028	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_J331028	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_J331028	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_J331028	.	ribosome_entry_site	1333	1344	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J331028	.	conserved_region	1337	1340	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J331028	.	CDS	4766	6982	.	+	0	ID=BBa_K137068;Name=BBa_K137068
BBa_J331028	.	sequence_alteration	4963	4963	.	+	0	ID=annotation1969499;Name=C->T;Parent=BBa_K137068
BBa_J331028	.	promoter	4001	4200	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_J331028	.	non_covalent_binding_site	4166	4200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_J331028	.	non_covalent_binding_site	4089	4126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_J331028	.	engineered_region	4001	4200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_J331028	.	promoter	4137	4142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_J331028	.	CDS	4001	4088	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_J331028	.	start_codon	4173	4173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_J331028	.	promoter	4161	4166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_J331028	.	ribosome_entry_site	4209	4220	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J331028	.	conserved_region	4213	4216	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J331028	.	CDS	227	1324	.	+	0	ID=BBa_K902052;Name=BBa_K902052
BBa_J331028	.	start_codon	227	229	.	+	0	ID=annotation2205133;Name=start;Parent=BBa_K902052
BBa_J331028	.	sequence_alteration	565	565	.	+	0	ID=annotation2205135;Name=G->A;Parent=BBa_K902052
BBa_J331028	.	CDS	227	1324	.	+	0	ID=annotation2205132;Name=DszB;Parent=BBa_K902052
BBa_J331028	.	stop_codon	1322	1324	.	+	0	ID=annotation2211179;Name=stop;Parent=BBa_K902052
BBa_J331028	.	sequence_alteration	413	415	.	+	0	ID=annotation2205134;Name=Y63F;Parent=BBa_K902052
BBa_J331028	.	sequence_alteration	1252	1252	.	+	0	ID=annotation2205136;Name=G->A;Parent=BBa_K902052
BBa_J331028	.	CDS	2631	3992	.	+	0	ID=BBa_K902050;Name=BBa_K902050
BBa_J331028	.	sequence_alteration	3641	3641	.	+	0	ID=annotation2205054;Name=G->A;Parent=BBa_K902050
BBa_J331028	.	start_codon	2631	2633	.	+	0	ID=annotation2205051;Name=start;Parent=BBa_K902050
BBa_J331028	.	CDS	2631	3992	.	+	0	ID=annotation2205093;Name=DszA;Parent=BBa_K902050
BBa_J331028	.	stop_codon	3990	3992	.	+	0	ID=annotation2211180;Name=stop;Parent=BBa_K902050
BBa_J331028	.	sequence_alteration	3308	3308	.	+	0	ID=annotation2205053;Name=G->A;Parent=BBa_K902050
BBa_J331028	.	sequence_alteration	3896	3896	.	+	0	ID=annotation2205055;Name=G->A;Parent=BBa_K902050
BBa_J331028	.	sequence_alteration	3269	3269	.	+	0	ID=annotation2205052;Name=G->A;Parent=BBa_K902050
BBa_J331028	.	CDS	4227	4739	.	+	0	ID=BBa_K902057;Name=BBa_K902057
BBa_J331028	.	stop_codon	4737	4739	.	+	0	ID=annotation2211176;Name=stop;Parent=BBa_K902057
BBa_J331028	.	CDS	4227	4739	.	+	0	ID=annotation2205092;Name=HpaC;Parent=BBa_K902057
BBa_J331028	.	start_codon	4227	4229	.	+	0	ID=annotation2205091;Name=start;Parent=BBa_K902057
BBa_J331028	.	CDS	1351	2604	.	+	0	ID=BBa_K804005;Name=BBa_K804005
>BBa_J331028
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgacaagccg
cgtcgaccccgcaaaccccggttcagaactcgattccgccatccgcgacacactgacctacagcaactgcccggtaccc
aacgctctgctcacggcatcggaatcgggcttcctcgacgccgccggcatcgaactcgacgtcctcagcggccagcagg
gcacggttcatttcaccttcgaccagcctgcctacacccgttttgggggtgagatcccgccactgctcagcgaggggtt
gcgggcacctgggcgcacgcgtctactcggcatcaccccgctcttggggcgccagggcttctttgtccgcgacgacagc
ccgatcacagcagccgccgaccttgccggacgtcgaatcggcgtctcggcctcggcaattcgcatcctgcgcggccagc
tgggcgactacctcgagttggatccctggcggcaaacgctggtagcgctgggctcgtgggaggcgcgcgccttgttgca
cacccttgagcacggtgaactgggtgtggacgacgtcgagctggtgccgatcagcagtcctggtgtcgatgttcccgct
gagcagctcgaagaatcggcgaccgtcaagggtgcggacctctttcccgatgtcgcccgcggtcaggccgcggtgttgg
ccagcggagacgttgacgccctgtacagttggctgccctgggccggggagttgcaagccaccggggcccgcccagtggt
ggatctcggcctcgatgagcgcaatgcctacgccagtgtgtggacggtcagcagcgggctggttcgccagcgacctggc
cttgttcaacgactggtcgacgcggccgtcgacgccgggctgtgggcacgcgatcattccgacgcggtgaccagcctgc
acgccgcgaacctgggcgtatcgaccggagcagtaggccagggcttcggcgccgacttccagcagcgtctggttccacg
cctggatcacgacgccctcgccctcctggagcgcacacagcaattcctgctcaccaacaacttgctacaggaacccgtc
gccctcgatcagtgggcggctccggaatttctgaacaacagcctcaatcgccaccgatagtactagagaaagaggagaa
atactagatgacactgtcacctgaaaagcagcacgttcgaccacgcgacgccgccgacaacgatcccgtcgcggttgcc
cgtgggctagccgaaaagtggcgagccaccgccgtcgagcgtgatcgcgccgggggttcggcaacagccgagcgcgaag
acctgcgcgcgagcgcgctgctgtcgctcctcgtcccgcgcgaatacggcggctggggcgcagactggcccaccgccat
cgaggtcgtccgcgaaatcgcggcagccgatggatctttgggacacctgttcggataccacctcaccaacgccccgatg
atcgaactgatcggctcgcaggaacaagaagaacacctgtacacccagatcgcgcagaacaactggtggaccggaaatg
cctccagcgagaacaacagccacgtgctggactggaaggtcagcgccaccccgaccgaagacggcggctacgtgctcaa
tggcacgaagcacttctacagcggcgccaaggggtcggacctgctgttcgtgttcggcgtcgtccaggatgattctccg
cagcagggtgcgatcattgctgccgctatcccgacatcgcgggctggcgttacgcccaacgacgactgggccgccatcg
gcatgcggcagaccgacagcggttccacggacttccacaacgtcaaggtcgagcctgacgaagtgctgggcgcgcccaa
cgccttcgttctcgccttcatacaatccgagcgcggcagcctcttccggcccatagcgcaattgatcttcgccaacgtc
tatctggggatcgcgcacggcgcactcgatgccgccagggagtacacccgtacccaggcgaggccctggacaccggccg
gtattcaacaggcaaccgaggatccctacaccatccgctcctacggtgagttcaccatcgcattgcagggagctgacgc
cgccgcccgtgaagcggcccacctgctacagacggtgtgggacaagggcgacgcgctcacccccgaggaccgcggcgaa
ctgatggtgaaggtctcgggagtcaaagcgttggccaccaacgccgccctcaacatcagcagcggcgtcttcgaggtga
tcggcgcgcgcggaacacatcccaggtacggtttcgaccgcttctggcgcaacgtgcgcacccactccctgcacgaccc
ggtgtcctacaagatcgccgacgtcggcaagcacaccttgaacggtcaatacccgattcccggcttcacctcctgatac
tagagaaagaggagaaatactagatgactcaacaacgacaaatgcatctggccggtttcttctcggccggcaatgtgac
tcatgcacatggggcgtggcggcacacggacgcgtcgaatgactttctgtcggggaagtactaccaacacatcgcccgt
actctggagcgcggcaagttcgatctgttgtttctgcctgacgggttggccgtcgaggacagctacggggacaacctgg
acaccggtgtcggcctgggcgggcagggtgcagtcgccttggagccggccagtgtggtcgcaaccatggccgcggtgac
cgagcacctgggtcttggggcaaccatttcggcgacctactatcccccgtatcacgttgctcgggtgttcgcgacgctc
gatcagttgtcagggggtcgggtgtcctggaacgtcgtcacctcgctcaacgacgctgaagcgcgcaacttcggcatta
atcagcatctggaacacgacgcccgctatgaccgcgccgatgagttcttggaagcggtcaagaaactctggaacagctg
ggacgaggacgccctcgtgctggacaaggcggccggcgtgttcgccgatcccgcgaaggtgcactacgtcgatcaccac
ggggagtggctgaatgtgcgcggacctctacaggtaccgcgttcacctcagggtgagccggtgatcctacaggccggcc
tgtcgccccggggtcggcgcttcgccgggaagtgggccgaggccgtcttcagtcttgcacccaacctcgaggtgatgca
ggccacctaccagggcatcaaagccgaggtcgacgctgcggggcgcgatcccgatcagacgaaaatcttcaccgccgtg
atgccggtactcggcgaaagccaggcggtggcacaggaacgactggaatatctcaacagtctggtccatccggaagtgg
gactgtcgacgctatccagtcacaccggcatcaacctggcggcgtaccctctcgacactccgatcaaggacatcctgcg
ggatctacaggatcggaatgtcccgacgcaactgcacatgttcgccgccgcaacgcacagcgaagagctcacgctggcg
gaaatgggtcggcgctatggaaccaacgtggggttcgttcctcagtgggccggtaccggggagcagatcgctgacgagc
tgatccgccacttcgagggcggcgccgcggatggtttcatcatctctccggccttcctgccgggctcctacgacgagtt
cgtcgaccaggtggttccggttctacaggatcgcggctacttccgcaccgagtaccagggcaacactctgcgcgaccac
ttgggtctgcgcgtaccacaactgcaaggacaaccttcatgatactagagcaatacgcaaaccgcctctccccgcgcgt
tggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtga
gttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggata
acaatttcacacatactagagaaagaggagaaatactagatgcaattagatgaacaacgcctgcgctttcgtgacgcaa
tggccagcctgtcggcagcggtaaatattatcaccaccgagggcgacgccggacaatgcgggattacggcaacggccgt
ctgctcggtcacggatacaccaccatcgctgatggtgtgcattaacgccaacagtgcgatgaacccggtttttcagggc
aacggtaagttgtgcgtcaacgtcctcaaccatgagcaggaactgatggcacgccacttcgcgggcatgacaggcatgg
cgatggaagagcgttttagcctctcatgctggcaaaaaggtccgctggcgcagccggtgctaaaaggttcgctggccag
tcttgaaggtgagatccgcgatgtgcaggcaattggcacacatctggtgtatctggtggagattaaaaacatcatcctc
agtgcagaaggtcacggacttatctactttaaacgccgtttccatccggtgatgctggaaatggaagctgcgatttaat
actagagaaagaggagaaatactagatgagcacgtcagacgatatccataacaccacagccactggcaaatgcccgttc
catcagggcggtcacgaccagagtgcgggggcgggcacaaccactcgcgactggtggccaaatcaacttcgtgttgacc
tgttaaaccaacattctaatcgttctaacccactgggtgaggactttgactaccgcaaagaatttagcaaattagatta
ctacggcctgaaaaaagatctgaaagccctgttgacagaatctcaaccgtggtggccagccgactggggcagttacgcc
ggtctgtttattcgtatggcctggcacggcgcggggacttaccgttcaatcgatggacgcggtggcgcgggtcgtggtc
agcaacgttttgcaccgctgaactcctggccggataacgtaagcctcgataaagcgcgtcgcctgttgtggccaatcaa
acagaaatatggtcagaaaatctcctgggccgacctgtttatcctcgcgggtaacgtggcgctagaaaactccggcttc
cgtaccttcggttttggtgccggtcgtgaagacgtctgggaaccggatctggatgttaactggggtgatgaaaaagcct
ggctgactcaccgtcatccggaagcgctggcgaaagcaccgctgggtgcaaccgagatgggtctgatttacgttaaccc
ggaaggcccggatcacagcggcgaaccgctttctgcggcagcagctatccgcgcgaccttcggcaacatgggcatgaac
gacgaagaaaccgtggcgctgattgcgggtggtcatacgctgggtaaaacccacggtgccggtccgacatcaaatgtag
gtcctgatccagaagctgcaccgattgaagaacaaggtttaggttgggcgagcacttacggcagcggcgttggcgcaga
tgccattacctctggtctggaagtagtctggacccagacgccgacccagtggagcaactatttcttcgagaacctgttc
aagtatgagtgggtacagacccgcagcccggctggcgcaatccagttcgaagcggtagacgcaccggaaattatcccgg
atccgtttgatccgtcgaagaaacgtaaaccgacaatgctggtgaccgacctgacgctgcgttttgatcctgagttcga
gaagatctctcgtcgtttcctcaacgatccgcaggcgttcaacgaagcctttgcccgtgcctggttcaaactgacgcac
agggatatggggccgaaatctcgctacatcgggccggaagtgccgaaagaagatctgatctggcaagatccgctgccgc
agccgatctacaacccgaccgagcaggacattatcgatctgaaattcgcgattgcggattctggtctgtctgttagtga
gctggtatcggtggcctgggcatctgcttctaccttccgtggtggcgacaaacgcggtggtgccaacggtgcgcgtctg
gcattaatgccgcagcgcgactgggatgtgaacgccgcagccgttcgtgctctgcctgttctggagaaaatccagaaag
agtctggtaaagcctcgctggcggatatcatagtgctggctggtgtggttggtgttgagaaagccgcaagcgccgcagg
tttgagcattcatgtaccgtttgcgccgggtcgcgttgatgcgcgtcaggatcagactgacattgagatgtttgagctg
ctggagccaattgctgacggtttccgtaactatcgcgctcgtctggacgtttccaccaccgagtcactgctgatcgaca
aagcacagcaactgacgctgaccgcgccggaaatgactgcgctggtgggcggcatgcgtgtactgggtgccaacttcga
tggcagcaaaaacggcgtcttcactgaccgcgttggcgtattgagcaatgacttcttcgtgaacttgctggatatgcgt
tacgagtggaaagcgaccgacgaatcgaaagagctgttcgaaggccgtgaccgtgaaaccggcgaagtgaaatttacgg
ccagccgtgcggatctggtgtttggttctaactccgtcctgcgtgcggtggcggaagtttacgccagtagcgatgccca
cgagaagtttgttaaagacttcgtggcggcatgggtgaaagtgatgaacctcgaccgtttcgacctgctggctgctaac
gacgaaaactacgctctggctgcttaataatactagagaaaaaaaaaccccgcccctgacagggcggggtttttttt