##gff-version 3
##sequence-region BBa_J331027 1 6331
BBa_J331027	.	terminator	6293	6331	.	+	0	ID=BBa_B1006;Name=BBa_B1006
BBa_J331027	.	polyA_site	6324	6331	.	+	0	ID=annotation1898430;Name=PolyA;Parent=BBa_B1006
BBa_J331027	.	stem_loop	6302	6323	.	+	0	ID=annotation1898429;Name=modified thr terminator;Parent=BBa_B1006
BBa_J331027	.	polyA_site	6293	6301	.	+	0	ID=annotation1898431;Name=PolyA;Parent=BBa_B1006
BBa_J331027	.	engineered_region	6293	6331	.	+	0	ID=annotation1898428;Name=B1006;Parent=BBa_B1006
BBa_J331027	.	CDS	1612	2601	.	+	0	ID=BBa_K902034;Name=BBa_K902034
BBa_J331027	.	start_codon	1612	1614	.	+	0	ID=annotation2205695;Name=ATG Start Codon;Parent=BBa_K902034
BBa_J331027	.	sequence_alteration	2268	2268	.	+	0	ID=annotation2205697;Name=NotI mutation (G --> A);Parent=BBa_K902034
BBa_J331027	.	stop_codon	2599	2601	.	+	0	ID=annotation2205696;Name=TAA Stop Codon;Parent=BBa_K902034
BBa_J331027	.	CDS	1262	1585	.	+	0	ID=BBa_K902033;Name=BBa_K902033
BBa_J331027	.	start_codon	1262	1264	.	+	0	ID=annotation2205029;Name=ATG start codon;Parent=BBa_K902033
BBa_J331027	.	stop_codon	1583	1585	.	+	0	ID=annotation2205036;Name=TAG stop codon;Parent=BBa_K902033
BBa_J331027	.	ribosome_entry_site	2610	2621	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J331027	.	conserved_region	2614	2617	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J331027	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J331027	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J331027	.	ribosome_entry_site	1244	1255	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J331027	.	conserved_region	1248	1251	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J331027	.	sequence_feature	4896	6284	.	+	0	ID=BBa_J331024;Name=BBa_J331024
BBa_J331027	.	sequence_feature	2628	4889	.	+	0	ID=BBa_J331025;Name=BBa_J331025
BBa_J331027	.	ribosome_entry_site	1594	1605	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J331027	.	conserved_region	1598	1601	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J331027	.	CDS	81	1235	.	+	0	ID=BBa_K902032;Name=BBa_K902032
BBa_J331027	.	stop_codon	1233	1235	.	+	0	ID=annotation2207749;Name=TGA Stop Codon;Parent=BBa_K902032
BBa_J331027	.	sequence_alteration	81	81	.	+	0	ID=annotation2207750;Name=G --> A;Parent=BBa_K902032
BBa_J331027	.	start_codon	81	83	.	+	0	ID=annotation2207748;Name=ATG Start Codon;Parent=BBa_K902032
BBa_J331027	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_J331027	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J331027	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J331027	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J331027	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J331027	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
>BBa_J331027
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatggcgaacgttgatgaggcaattttaaaaagagtaaaaggctgggcgccctacgtggatgcgaagctaggctttcgc
aatcattggtacccggtgatgttttcgaaagagatcgacgagggcgagccgaagacactaaaactgctcggtgagaact
tgctcgtcaatcgtatcgatgggaagctgtattgcctcaaggaccgctgcctgcatcgcggcgtccagttgtcggtcaa
agtcgagtgcaaaacgaagtcgacgatcacatgctggtaccacgcgtggacctatcgctgggaagacggcgttctgtgc
gacatcttgacgaatccgacaagcgcacagatcggtcgacaaaagctgaaaacttacccagtgcaggaagccaagggct
gcgtcttcatttatcttggcgatggcgaccctcctcccttggcccgcgatacgccacccaatttccttgacgatgacat
ggaaatcctcgggaagaaccaaatcatcaagtctaactggcgcctcgctgtggaaaacggtttcgatccgagccacatt
tatattcacaaagactcaattctggtcaaggacaacgatcttgccttgccactaggtttcgcgccaggaggggatcgaa
agcaacaaactcgtgtggttgacgatgacgtcgtcggacgcaagggtgtttacgatcttattggcgaacatggggtccc
agtgtttgagggaactatcgggggcgaagtggtccgcgaaggtgcctacggcgaaaaaattgtagcgaacgatatctcc
atttggctcccgggtgttctcaaggtcaatccgttccccaatccggacatgatgcagttcgagtggtacgtgccgattg
acgaaaacacacactattacttccaaactcttggcaaaccatgtgccaatgacgaggaacggaagaattacgaacaaga
gttcgaaagcaagtggaaaccgatggcgctcgaaggattcaacaacgatgacatctgggctcgcgaagctatggtggat
ttctacgccgatgataaaggctgggtcaacgagattttgttcgaggtggacgaggctatcgtggcatggcgcaagctgg
cgagcgaacacaatcagggtattcagacccaagcgcacgtttcgggctgatactagagaaagaggagaaatactagatg
aaccaaatttggttgaaagtatgtgctgcatctgacatgcaacctggcacgatacgtcgcgtcaaccgcgtaggtgctg
cacctctcgcagtctatcgtgttggcgatcagttctacgccactgaagatacgtgcacgcatggtattgcttcgctttc
ggaagggacactcgatggtgacgtgattgaatgtccctttcacggcggcgccttcaatgtttgtaccggcatgccggca
tcaagtccatgtacagtgccgctaggagtgttcgaggtagaagtcaaagagggcgaagtttatgtcgccggagaaaaga
agtagtactagagaaagaggagaaatactagatgtaccaactcaaaattgaagggcaagcgccagggacctgcggctca
gggaagagcctgttggtctcagcacttgctaatggtatcggatttccgtacgagtgtgcatcgggaggttgcggagtat
gcaaattcgagttactcgaagggaatgtccaatcaatgtggccggatgctccaggactttcttcgcgagatcgtgagaa
gggcaaccgccatcttgcatgccagtgcgttgcgctctcagacctgcggatcaaagtcgcagtgcaggacaagtacgtc
ccaacgattccaatctcaagaatggaagcggaagttgttgaggtccgggcgctaactcatgacctgctgtccgtgcgat
tacgcactgatgggccagcaaatttcctccccggccagttctgcctagtagaggcagagcagttgccaggcgtggttcg
cgcatattcaatggcgaatttaaagaaccccgaaggcatatgggagttctatattaagagggtacccacaggacgattt
agtccttggcttttcgaaaatagaaaagaaggcgctcgtctatttttgacgggaccaatgggcacatctttcttccgtc
cagggaccggccgaaagagtctttgcattggcggcggtgccgggctctcgtatgcagccgctattgcacgcgcctcgat
gcgcgaaacagacaagccggtaaagttgttctacggctcaagaactccgcgcgacgctgttcggtggatcgatatcgac
atcgatgaggacaagcttgaggtcgtccaggcagttacggaagacacggatagcctttggcaagggcccactggtttta
ttcatcaggttgtcgacgcagcgctgcttgaaaccctaccggaatacgaaatttatcttgccggtccaccgcctatggt
cgacgctactgtccgtatgctgctcggcaagggtgttccacgcgatcaaattcattttgacgcatttttctaatactag
agaaagaggagaaatactagatgaccgacaagtcgaatgtggaattttccgagcgcgaggcgctgttcttccactcgac
gggacgccccggcaagatcgagatcatcgcctcgaagccgatggcgacccagcgcgacctcagcctcgcctactcgccg
ggcgtcgcggtgccggtgcgcgccatcgcgaaagacccggcgaccgcctatgactataccgccaagggcaatctggtcg
cggtgatctccaacggcacggcgatcctggggctgggcaatctgggcgcgctggcgtccaagccggtgatggagggcaa
gtcggtcctcttcaagcgcttcgccgacgtcgacagcatcgacatcgaactcaagaccgaggatgtcgacaagttcatc
gacgcggtcgagctgatggagccgaccttcggcggcatcaacctggaggacatcaaggccccggaatgcttcatcatcg
aacagacgctgaaggagcggatgaacattccggtcttccatgacgaccagcacggcaccgcgatcatcgcggcggcggg
cgtcatcaacgcggcgatgctgaccggccgcgagatgaaggacatgaaggtggtggtgaacggcgcgggcgcggcgtcc
atctcctgcaccgaactgatcaaggcgctgggcgtgcagcatgacaatgtgatcatgtgcgacagcaagggcgtgatct
accagggccgcaccgagggcatgaaccagtggaagtcggcccatgcggtgaagaccgacgcccgttcgctggccgaagc
ggtgaagggcgcggacgtgttcctgggcctctccgtcgccggtgcgatgacgcaggacatggtgaagtccatggcggac
aagccgatcatcttcgccatggcgaaccccgacccggaaatcgcgccgcccgacgccaaggcggtgcggcccgacgcca
tcgtcgccacgggccggtcggactatcccaaccaggtcaacaatgtgctgggcttccccttcatcttccgcggcgcgct
ggacgtgcgggccaccgggatcaacgaggcgatgaagctggccgccgccaaggccatcgccgaactggcgcgggaacag
gtgccggaggaagtcgccaaggcctatggccgctcgcacagcttcggcccggactatatcatccccgcgcccttcgacc
cgcgcctgatggaggtcgtgccctccgccgtcgcgcaggcggcgatggagaccggcgtcgcgcagcggcccatcgcgga
catggcggcctatcgccaatccttgaaggggcggctcaacccgaccacctccgtcctcaccaccgcctatgaggtggcg
aaggccaatcccaagcgcgtcgtcttcgccgaggcggaagaggaagtagtgctgcgcgccgccatccagttccgcaccc
ttggctatggcatccccgtgctggtcgggcgcgcccatgtctatgacaagctcaaggaactgggcgtggaggatccgga
aagctacgaactgcacaacagcgtcaactcgccgctggtgccggaaatggtggacacgctctacaagcggctccagcgc
cggggctatctgcgccgcgattgcgagcggatggtcaatcgcgaccgtaacatcttcggctcgctgctggtgaagatgg
gccatgccgacgcgatgctgaccggcatgacccgtcccttcggacagacgctgcgcgaagtgaagcgggtgctggaccc
ggcggcgggccgcacccccttcggcatccatgtgctggtgacgaaggacaagaccgtgttcctggccgacaccacggtg
aacgagcgtcccacggcccaggaactggccgacatcgcggaaggtacggtggcggtcgcccggcgcatgggccatgatc
cgcgcgtcgcattcctgtcctattccaatttcggcaacccgccgggcggcttcctggaaaatgtccgcgacgcggtgaa
ggtgctggaagaccgcgaggtcgacttcgaattcgaaggggagatgaccgccgatgcggcgctcaacccgacggtgatg
aagaattatcccttcagccgcctgtccggcccggccaatatattggtgatgccggggcttcagtcggccaatatctctg
ccaagctgctgcgcgaactgggcggcgcgaccatgatcgggccgatgctggtcggcatggacaagtcggtgcagatcgc
caccatggcgtccaacgcatcggaattgctgacgctggcggtgctggcttcggccggcgtggcggtctgatactagatg
agtggtgcaagaaacgtcgagcagtggcaagacttcatcggcagctgcctggactttcgtcccgcggacggggtgttcc
gtatcgcccgggacatgttcaccgaacccgagctgttcgacctggagatggagctgatcttcgagaagaattggatcta
cgcctgtcacgaaagcgagctgcccaacaaccacgacttcatcacgatgcgcgccgggcgccagccgatgatcatcacc
cgcgacggcgagggccggctcaatgccctggtcaacgcctgccagcaccgcggcaccaccctgacccgggttggcaagg
gcaaccagtccaccttcacctgccccttccatgcctggtgctacaagagcgacgggcgcctggtgaaggtcaaggcgcc
aggggaatacgacgagggcttcgacaaggccacccgcggcctgaagaaggcccgcatcgagagctacaagggcttcgtc
ttcatcagcctggacgtggccgccgacaattccctggaagacttcctcggcgacgccaggatcttcttcgacatgatgg
tcgcccagtcccccgacggtgagctggaggtgctgccgggcaagtccgcctacacctacgacggcaactggaagctgca
gaacgagaacggcctggacggttatcacgtcagcaccgtgcactacaactacgtggccacggtgcagcaccgccagcag
gtcaacgccgacagcggcgccggcagcagcgccaccctggactacagcaagctcggcgccggcgacgccagcaccgacg
atggctggttcgccttcgagaacggccacagcgtgctgttcagcgaaatgcccaacccggcggtgcgctccggctacgc
cagcatcatgccgcgcctggtggccgagcacggccaggccaaggccgagtggatgatgcaccgcctgcgcaacctgaac
atctaccccagcctgttcttcctcgaccagatcagttcccagttgcggatcatccgccccatcgcctggaacaagaccg
agatcatcagccagtgcatcggggtcaagggcgagagcgacgccgaccgggaaaaccgcatccgccagttcgaggattt
cttcaacgtctcgggcatgggcacgccggacgacctggtggagttccgcgaggcccagcgcggcttccaggggcgcctg
gagcgctggagcgacatctcccgcgggcgccagcattgggtcagcgggcccaccgccaacagcgagaacctgggcatcc
agccggccatgaccggcaccgagatcacccatgaggggctgtacgtcaaccagcaccgcaactggcagaaattcctcct
cgacggcctgcagcgccggcccctgaaactgcgtgaggtgtgatactagagaaaaaaaaaccccgcccctgacagggcg
gggtttttttt