##gff-version 3
##sequence-region BBa_J331021 1 6455
BBa_J331021	.	promoter	2141	3192	.	+	0	ID=BBa_K228004;Name=BBa_K228004
BBa_J331021	.	promoter	3122	3190	.	+	0	ID=annotation2075287;Name=pSal;Parent=BBa_K228004
BBa_J331021	.	promoter	3044	3133	.	+	0	ID=annotation2075286;Name=Pr promoter;Parent=BBa_K228004
BBa_J331021	.	CDS	2141	3043	.	+	0	ID=annotation2075285;Name=NahR;Parent=BBa_K228004
BBa_J331021	.	engineered_region	1	2132	.	+	0	ID=BBa_K1031610;Name=BBa_K1031610
BBa_J331021	.	CDS	1275	1997	.	+	0	ID=annotation2360411;Name=sfGFP;Parent=BBa_K1031610
BBa_J331021	.	stem_loop	2004	2132	.	+	0	ID=annotation2360412;Name=B0015;Parent=BBa_K1031610
BBa_J331021	.	CDS	211	1113	.	+	0	ID=annotation2360409;Name=NahR;Parent=BBa_K1031610
BBa_J331021	.	promoter	1192	1262	.	+	0	ID=annotation2360410;Name=pSal ;Parent=BBa_K1031610
BBa_J331021	.	engineered_region	3201	6455	.	+	0	ID=BBa_J45700;Name=BBa_J45700
BBa_J331021	.	terminator	4452	4492	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J45700
BBa_J331021	.	engineered_region	4452	4492	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J331021	.	stop_codon	4485	4485	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J331021	.	stem_loop	4459	4478	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J331021	.	polyA_site	4479	4492	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J331021	.	CDS	3282	4355	.	+	0	ID=BBa_J45004;Name=BBa_J45004;Parent=BBa_J45700
BBa_J331021	.	stop_codon	4353	4355	.	+	0	ID=annotation1880520;Name=stop codon;Parent=BBa_J45004
BBa_J331021	.	start_codon	3282	3284	.	+	0	ID=annotation1880519;Name=start codon;Parent=BBa_J45004
BBa_J331021	.	CDS	3282	4355	.	+	0	ID=annotation1880517;Name=BMST1;Parent=BBa_J45004
BBa_J331021	.	terminator	6327	6406	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J45700
BBa_J331021	.	engineered_region	6327	6406	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J331021	.	stem_loop	6338	6381	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J331021	.	terminator	6415	6455	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J45700
BBa_J331021	.	engineered_region	6415	6455	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J331021	.	stop_codon	6448	6448	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J331021	.	stem_loop	6422	6441	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J331021	.	polyA_site	6442	6455	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J331021	.	terminator	4364	4443	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J45700
BBa_J331021	.	engineered_region	4364	4443	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J331021	.	stem_loop	4375	4418	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J331021	.	ribosome_entry_site	4564	4576	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_J45700
BBa_J331021	.	ribosome_entry_site	4570	4573	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_J331021	.	ribosome_entry_site	4564	4576	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_J331021	.	engineered_region	4564	4576	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_J331021	.	promoter	3201	3254	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_J45700
BBa_J331021	.	promoter	3220	3225	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J331021	.	non_covalent_binding_site	3226	3244	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J331021	.	engineered_region	3201	3254	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J331021	.	promoter	3243	3248	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J331021	.	non_covalent_binding_site	3201	3219	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J331021	.	promoter	4501	4554	.	+	0	ID=BBa_R0011;Name=BBa_R0011;Parent=BBa_J45700
BBa_J331021	.	promoter	4543	4548	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_J331021	.	promoter	4520	4525	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_J331021	.	operator	4526	4542	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_J331021	.	engineered_region	4501	4554	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_J331021	.	operator	4503	4519	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_J331021	.	ribosome_entry_site	3263	3275	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_J45700
BBa_J331021	.	ribosome_entry_site	3269	3272	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_J331021	.	ribosome_entry_site	3263	3275	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_J331021	.	engineered_region	3263	3275	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_J331021	.	CDS	4583	6318	.	+	0	ID=BBa_J45017;Name=BBa_J45017;Parent=BBa_J45700
BBa_J331021	.	start_codon	4583	4585	.	+	0	ID=annotation1897455;Name=pchB start codon;Parent=BBa_J45017
BBa_J331021	.	stop_codon	4886	4888	.	+	0	ID=annotation1897456;Name=pchB stop codon;Parent=BBa_J45017
BBa_J331021	.	stop_codon	6313	6318	.	+	0	ID=annotation1897457;Name=pchA double TAA stop codon;Parent=BBa_J45017
BBa_J331021	.	CDS	4885	6318	.	+	0	ID=annotation1961297;Name=PchA;Parent=BBa_J45017
BBa_J331021	.	start_codon	4885	4887	.	+	0	ID=annotation1897454;Name=pchA start codon;Parent=BBa_J45017
BBa_J331021	.	point_mutation	5022	5022	.	+	0	ID=annotation1897453;Name=g -> a to eliminate PstI site;Parent=BBa_J45017
BBa_J331021	.	CDS	4583	4888	.	+	0	ID=annotation1961296;Name=PchB;Parent=BBa_J45017
>BBa_J331021
ggccgctgcgatcccgcgaagaaccaaaaaagctcgacagagggcgcggtcattttaggtcgggcggatcggcgccgcc
ggctcggctggtgtgccgcacagcaccgcctacgtgagctgccagttgatgaacttcccccgttgccagctagggcgca
agcgggctgtataagatcactgcccatcacattgatcggctcggattttttctcaatccgtaaacaggtcaaacatcag
ttgccgcaaccaaatattggctaggtccttgtggtacttcgcatgccagaacatgttgatggctatttcaggcaagacg
actgggtgcggcaaggcgcttaggccgaagggctccacgcagcagtcggctaaacgtatcggcacagtggcgagcagat
cggtgcgctggaggatgtggccaacggcggcgaagtgcggcacttccagacggatgtcgcgccggatgccgacccgtgt
catgtacgtgtccacctcgccgtggccggtgccagcggcgatgacacgcacgtggccgtaggaacagaagcgctccaga
gtcaggggttcgcgggtgactggatggtccttgcgacataggcacacgtagtgattctggagcagccggcgctgaaaga
agccagtttgcagattgggaagcaggcccacggccaagtccacggttccgttctgcaaggcctgcatcaggctcatcga
actgtcgcgcaccgtactgatcacgcaattgggggcctggtgagccagcacatccatcagccgcggcatgaagtagatc
tcgccaatgtcggtcatggccagggtgaaggtacgctcgctggtcagcggatcgaagctttcatggtgctgtagggcgt
tgcgcagtgcgtgcatggccgaagtgacgggctcggccagatgcgcggcatagggtgtgggttccattccctgatgtgt
gcgcacgaagagtgggtcctgtagcgaggtgcgcaggcgtttcagcgcattgctcacggcaggctgggtcaggcccagg
ttctccgcagtgatagagacgcgtctgtcgaccagcaactggttgaacaccaccagcaggtttaaatccaggtcacgca
gttccatggggcctcgcttgggttattgctggtgcccggccgggcgcaatattcatgttgatgatttattatatatcga
gtggtgtatttatcaatattgtttgctccgttatcgttattaacaagtcatcaataaagccatcacgagtaccatagag
gatcactagaatgcgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaac
ggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtacta
ctggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccgga
ccatatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggat
gacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattg
actttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtttacatcaccgccga
taaacaaaaaaatggcattaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcac
taccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgt
ctaaagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatgga
tgaactgtacaaatgatgataaactagaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtt
ttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatat
actagagtcaatccgtaaacaggtcaaacatcagttgccgcaaccaaatattggctaggtccttgtggtacttcgcatg
ccagaacatgttgatggctatttcaggcaagacgactgggtgcggcaaggcgcttaggccgaagggctccacgcagcag
tcggctaaacgtatcggcacagtggcgagcagatcggtgcgctggaggatgtggccaacggcggcgaagtgcggcactt
ccagacggatgtcgcgccggatgccgacccgtgtcatgtacgtgtccacctcgccgtggccggtgccagcggcgatgac
acgcacgtggccgtaggaacagaagcgctccagagtcaggggttcgcgggtgactggatggtccttgcgacataggcac
acgtagtgattctggagcagccggcgctgaaagaagccagtttgcagattgggaagcaggcccacggccaagtccacgg
ttccgttctgcaaggcctgcatcaggctcatcgaactgtcgcgcaccgtactgatcacgcaattgggggcctggtgagc
cagcacatccatcagccgcggcatgaagtagatctcgccaatgtcggtcatggccagggtgaaggtacgctcgctggtc
agcggatcgaagctttcatggtgctgtagggcgttgcgcagtgcgtgcatggccgaagtgacgggctcggccagatgcg
cggcatagggtgtgggttccattccctgatgtgtgcgcacgaagagtgggtcctgtagcgaggtgcgcaggcgtttcag
cgcattgctcacggcaggctgggtcaggcccaggttctccgcagtgatagagacgcgtctgtcgaccagcaactggttg
aacaccaccagcaggtttaaatccaggtcacgcagttccatggggcctcgcttgggttattgctggtgcccggccgggc
gcaatattcatgttgatgatttattatatatcgagtggtgtatttatcaatattgtttgctccgttatcgttattaaca
agtcatcaataaagccatcacgagtaccatagtactagagtccctatcagtgatagagattgacatccctatcagtgat
agagatactgagcactactagagtcacacaggaaagtactagatggaagttgttgaagttcttcacatgaatggaggaa
atggagacagtagctatgcaaacaattctttggttcagcaaaaggtgattctcatgacaaagccaataactgagcaagc
catgattgatctctacagcagcctctttccagaaaccttatgcattgcagatttgggttgttctttgggagctaacact
ttcttggtggtctcacagcttgttaaaatagtagaaaaagaacgaaaaaagcatggttttaagtctccagagttttatt
ttcacttcaatgatcttcctggcaatgattttaatacactttttcagtcactgggggcatttcaagaagatttgagaaa
gcatataggggaaagctttggtccatgttttttcagtggagtgcctggttcattttatactagacttttcccttccaaa
agtttacattttgtttactcctcctacagtctcatgtggctatctcaggtgcctaatgggattgaaaataacaagggaa
acatttacatggcaagaacaagccctctaagtgttattaaagcatactacaagcaatatgaaatagatttttcaaattt
tctcaagtaccgttcagaggaattgatgaaaggtggaaagatggtgttaacactcctaggtagagaaagtgaggatcct
actagcaaagaatgctgttacatttgggagcttctagccatggccctcaataagttggttgaagagggattgataaaag
aagagaaagtagatgcattcaatattcctcaatacacaccatcaccagcagaagtaaagtacatagttgagaaggaagg
atcattcaccattaatcgcttggaaacatcaagagttcattggaatgcttctaataatgagaagaatggtggttacaat
gtgtcaaggtgcatgagagctgtggctgagcctttgcttgtcagccactttgacaaggaattgatggatttagtgttcc
acaagtacgaagagattgtttctgattgcatgtccaaagagaatactgagtttataaatgtcatcatctccttgaccaa
aataaattaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttg
tttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaat
tgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagtcacacaggaaagtactag
atgaaaactcccgaagactgcaccggcctggcggacatccgcgaggccatcgaccggatcgacctggatatcgtccagg
ccctcggccgccgcatggactacgtcaaggcggcgtcgcgcttcaaggccagcgaggcggcgattccggcgcccgagcg
ggtcgccgcgatgctccccgagcgcgcccgctgggccgaggaaaacggactcgacgcgcccttcgtcgagggactgttc
gcgcagatcatccactggtacatcgccgagcagatcaagtactggcgccagacacggggtgccgcatgagccggctggc
gcccctgagccagtgcctgcacgccttgcgcggcaccttcgagcgcgccatcggccaggcgcaggcgctcgatcgtccg
gtgctggtggcggcatcgttcgagatcgacccattggacccgctacaggtattcggtgcctgggacgaccggcaaacgc
cctgcctgtactgggaacagcccgagctggcgttcttcgcctggggctgcgccctggagctgcaaggccacggcgaaca
gcgcttcgcccggatcgaggaaaactggcaattgctctgcgccgacgccgtggtcgagggcccgctggcgccgcgcctg
tgcggcggattccgcttcgatccgcgcggcccgcgcgaggaacactggcaagccttcgccgatgccagcctgatgctcg
ccggcatcaccgtgctgcgcgagggcgaacgctaccgggtactctgccaacacctggccaagcccggcgaagatgccct
ggccctggccgcctaccactgctcggcgctactgcgcctgaggcagccggccagacgccggccctcggggccgaccgct
ggcgcgcagggcgacgcttcggcgcaggagcgcaggcaatgggaagccaaggtgagcgacgcggtaagcagtgtccgcc
agggacgcttcggcaaggtcgtgctggcccgcacccaggcccggcctctcggcgacatcgagccgtggcaggtcatcga
acacctgcgtctgcaacatgccgacgcccagctgttcgcctgtcgccgcggcaacgcctgcttcctcggcgcctccccg
gaacgcctggtccgcattcgcgccggcgaggcactcacccatgccctggccgggaccatcgcccgcggcggcgatgccc
aggaagatgcgcggctcggacaggccctgctggacagcgccaaggacaggcacgaacaccagttggtggtggaggcgat
ccgtacggccctggaacccttcagcgaggtgctggaaatccccgatgcgcccggcctgaaacgactggcgcgagtccag
cacctgaacacgccgatccgcgcccgcctcgctgacgcaggcggcatcctgcggctgctacaagcgctgcatccgaccc
ccgcggtgggcggctacccacgcagcgcggcgctggactacatccgccagcacgaagggatggaccgcggctggtacgc
cgcgccgctgggctggctcgacggcgaaggcaacggcgatttcctggtggcgctgcgctcggccctgctcacgccgggc
cggggctacctgttcgccggctgcggtctggtaggcgattcggaaccggcccacgagtatcgcgaaacctgccttaagc
tcagtgccatgcgggaagctctatccgccataggcggcctggacgaagtgcccttgcagcgcggcgtcgcctaataata
ctagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaa
cgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata