##gff-version 3
##sequence-region BBa_J329060 1 2089
BBa_J329060	.	engineered_region	1	1012	.	+	0	ID=BBa_J329050;Name=BBa_J329050
BBa_J329060	.	CDS	62	850	.	+	0	ID=BBa_C0060;Name=BBa_C0060;Parent=BBa_J329050
BBa_J329060	.	start_codon	62	64	.	+	0	ID=annotation1754;Name=start;Parent=BBa_C0060
BBa_J329060	.	engineered_tag	851	875	.	+	0	ID=annotation2213987;Name=Help:Barcodes;Parent=BBa_C0060
BBa_J329060	.	tag	812	844	.	+	0	ID=annotation1756;Name=LVA;Parent=BBa_C0060
BBa_J329060	.	CDS	62	811	.	+	0	ID=annotation1757;Name=aiiA;Parent=BBa_C0060
BBa_J329060	.	engineered_region	62	850	.	+	0	ID=annotation7037;Name=BBa_C0060;Parent=BBa_C0060
BBa_J329060	.	stop_codon	845	850	.	+	0	ID=annotation1755;Name=2;Parent=BBa_C0060
BBa_J329060	.	terminator	884	1012	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_J329050
BBa_J329060	.	terminator	972	1012	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_J329060	.	polyA_site	999	1012	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J329060	.	engineered_region	972	1012	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J329060	.	stop_codon	1005	1005	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J329060	.	stem_loop	979	998	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J329060	.	terminator	884	963	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_J329060	.	engineered_region	884	963	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J329060	.	stem_loop	895	938	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J329060	.	engineered_region	1	55	.	+	0	ID=BBa_K880005;Name=BBa_K880005;Parent=BBa_J329050
BBa_J329060	.	promoter	1	35	.	+	0	ID=BBa_J23100;Name=BBa_J23100;Parent=BBa_K880005
BBa_J329060	.	ribosome_entry_site	44	55	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K880005
BBa_J329060	.	conserved_region	48	51	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J329060	.	engineered_region	1021	2089	.	+	0	ID=BBa_J04450;Name=BBa_J04450
BBa_J329060	.	CDS	1247	1927	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_J04450
BBa_J329060	.	engineered_tag	1928	1952	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_J329060	.	CDS	1247	1921	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_J329060	.	promoter	1021	1220	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_J04450
BBa_J329060	.	non_covalent_binding_site	1109	1146	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_J329060	.	promoter	1181	1186	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_J329060	.	start_codon	1193	1193	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_J329060	.	engineered_region	1021	1220	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_J329060	.	non_covalent_binding_site	1186	1220	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_J329060	.	CDS	1021	1108	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_J329060	.	promoter	1157	1162	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_J329060	.	terminator	1961	2040	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J04450
BBa_J329060	.	engineered_region	1961	2040	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J329060	.	stem_loop	1972	2015	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J329060	.	terminator	2049	2089	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J04450
BBa_J329060	.	polyA_site	2076	2089	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J329060	.	engineered_region	2049	2089	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J329060	.	stop_codon	2082	2082	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J329060	.	stem_loop	2056	2075	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J329060	.	ribosome_entry_site	1229	1240	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J04450
BBa_J329060	.	conserved_region	1233	1236	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_J329060
ttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaatactagatgacagtaaagaagctt
tatttcgtcccagcaggtcgttgtatgttggatcattcgtctgttaatagtacattaacaccaggagaattattagact
taccggtttggtgttatcttttggagactgaagaaggacctattttagtagatacaggtatgccagaaagtgcagttaa
taatgaaggtctttttaacggtacatttgtcgaagggcaggttttaccgaaaatgactgaagaagatagaatcgtgaat
attttaaaacgggttggttatgagccggaagaccttctttatattattagttctcacttgcattttgatcatgcaggag
gaaatggcgcttttataaatacaccaatcattgtacagcgtgctgaatatgaggcggcgcagcatagcgaagaatattt
gaaagaatgtatattgccgaatttaaactacaaaatcattgaaggtgattatgaagtcgtaccaggagttcaattattg
catacaccaggccatactccagggcatcaatcgctattaattgagacagaaaaatccggtcctgtattattaacgattg
atgcatcgtatacgaaagagaattttgaaaatgaagtgccatttgcgggatttgattcagaattagctttatcttcaat
taaacgtttaaaagaagtggtgatgaaagagaagccgattgttttctttggacatgatatagagcaggaaaggggatgt
aaagtgttccctgaatatatagctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgta
gatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttg
tcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagcaatacg
caaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagt
gagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgt
tgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggcttcctccgaagac
gttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaag
gtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttggga
catcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactg
tccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcct
ccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaa
aaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgt
ctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgg
gtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgc
tgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataa
aacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacac
tggctcaccttcgggtgggcctttctgcgtttata