##gff-version 3
##sequence-region BBa_J329031 1 4026
BBa_J329031	.	engineered_region	2958	4026	.	+	0	ID=BBa_J04450;Name=BBa_J04450
BBa_J329031	.	terminator	3986	4026	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J04450
BBa_J329031	.	stem_loop	3993	4012	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J329031	.	engineered_region	3986	4026	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J329031	.	stop_codon	4019	4019	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J329031	.	polyA_site	4013	4026	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J329031	.	CDS	3184	3864	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_J04450
BBa_J329031	.	CDS	3184	3858	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_J329031	.	engineered_tag	3865	3889	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_J329031	.	terminator	3898	3977	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J04450
BBa_J329031	.	engineered_region	3898	3977	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J329031	.	stem_loop	3909	3952	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J329031	.	ribosome_entry_site	3166	3177	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J04450
BBa_J329031	.	conserved_region	3170	3173	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J329031	.	promoter	2958	3157	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_J04450
BBa_J329031	.	engineered_region	2958	3157	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_J329031	.	non_covalent_binding_site	3123	3157	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_J329031	.	CDS	2958	3045	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_J329031	.	non_covalent_binding_site	3046	3083	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_J329031	.	promoter	3094	3099	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_J329031	.	start_codon	3130	3130	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_J329031	.	promoter	3118	3123	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_J329031	.	engineered_region	2036	2949	.	+	0	ID=BBa_I763020;Name=BBa_I763020
BBa_J329031	.	terminator	2909	2949	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I763020
BBa_J329031	.	stem_loop	2916	2935	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J329031	.	engineered_region	2909	2949	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J329031	.	stop_codon	2942	2942	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J329031	.	polyA_site	2936	2949	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J329031	.	engineered_region	2054	2812	.	+	0	ID=BBa_J04031;Name=BBa_J04031;Parent=BBa_I763020
BBa_J329031	.	stop_codon	2807	2812	.	+	0	ID=annotation1507242;Name=Double Stop;Parent=BBa_J04031
BBa_J329031	.	engineered_region	2054	2773	.	+	0	ID=annotation1507202;Name=BBa_E0040;Parent=BBa_J04031
BBa_J329031	.	start_codon	2054	2056	.	+	0	ID=annotation1507246;Name=Start;Parent=BBa_J04031
BBa_J329031	.	tag	2768	2806	.	+	0	ID=annotation1507235;Name=LVA;Parent=BBa_J04031
BBa_J329031	.	terminator	2821	2900	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I763020
BBa_J329031	.	engineered_region	2821	2900	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J329031	.	stem_loop	2832	2875	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J329031	.	ribosome_entry_site	2036	2047	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I763020
BBa_J329031	.	conserved_region	2040	2043	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J329031	.	promoter	1973	2027	.	+	0	ID=BBa_R0062;Name=BBa_R0062
BBa_J329031	.	non_covalent_binding_site	1973	1992	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_J329031	.	promoter	1992	1997	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_J329031	.	engineered_region	1973	2027	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_J329031	.	promoter	2014	2019	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_J329031	.	start_codon	2025	2025	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_J329031	.	engineered_region	1	1964	.	+	0	ID=BBa_K546000;Name=BBa_K546000
BBa_J329031	.	engineered_region	1167	1964	.	+	0	ID=BBa_K082014;Name=BBa_K082014;Parent=BBa_K546000
BBa_J329031	.	terminator	1836	1964	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K082014
BBa_J329031	.	terminator	1924	1964	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_J329031	.	stem_loop	1931	1950	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J329031	.	engineered_region	1924	1964	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J329031	.	stop_codon	1957	1957	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J329031	.	polyA_site	1951	1964	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J329031	.	terminator	1836	1915	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_J329031	.	engineered_region	1836	1915	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J329031	.	stem_loop	1847	1890	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J329031	.	engineered_region	1167	1827	.	+	0	ID=BBa_C0261;Name=BBa_C0261;Parent=BBa_K082014
BBa_J329031	.	ribosome_entry_site	1167	1178	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_C0261
BBa_J329031	.	conserved_region	1171	1174	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J329031	.	CDS	1185	1802	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_C0261
BBa_J329031	.	tag	1764	1795	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_J329031	.	engineered_tag	1803	1827	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_J329031	.	engineered_region	1185	1802	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_J329031	.	CDS	1185	1763	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_J329031	.	engineered_region	1	1158	.	+	0	ID=BBa_F2621;Name=BBa_F2621;Parent=BBa_K546000
BBa_J329031	.	terminator	967	1046	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_F2621
BBa_J329031	.	engineered_region	967	1046	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J329031	.	stem_loop	978	1021	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J329031	.	promoter	1104	1158	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_F2621
BBa_J329031	.	non_covalent_binding_site	1104	1123	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_J329031	.	promoter	1123	1128	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_J329031	.	engineered_region	1104	1158	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_J329031	.	promoter	1145	1150	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_J329031	.	start_codon	1156	1156	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_J329031	.	terminator	1055	1095	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_F2621
BBa_J329031	.	stem_loop	1062	1081	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J329031	.	engineered_region	1055	1095	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J329031	.	stop_codon	1088	1088	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J329031	.	polyA_site	1082	1095	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J329031	.	ribosome_entry_site	160	171	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_F2621
BBa_J329031	.	conserved_region	164	167	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J329031	.	CDS	178	933	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_F2621
BBa_J329031	.	engineered_tag	934	958	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_J329031	.	engineered_region	178	179	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_J329031	.	CDS	178	927	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_J329031	.	point_mutation	351	351	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_J329031	.	engineered_region	178	933	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_J329031	.	point_mutation	669	669	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_J329031	.	promoter	1	151	.	+	0	ID=BBa_R0063;Name=BBa_R0063;Parent=BBa_F2621
BBa_J329031	.	promoter	89	94	.	+	0	ID=annotation2053;Name=-35;Parent=BBa_R0063
BBa_J329031	.	non_covalent_binding_site	130	149	.	+	0	ID=annotation2055;Name=Putative LuxR/HSL;Parent=BBa_R0063
BBa_J329031	.	engineered_region	1	151	.	+	0	ID=annotation7071;Name=BBa_R0063;Parent=BBa_R0063
BBa_J329031	.	start_codon	128	128	.	+	0	ID=annotation2054;Name=start;Parent=BBa_R0063
BBa_J329031	.	promoter	115	122	.	+	0	ID=annotation2052;Name=-10;Parent=BBa_R0063
BBa_J329031	.	non_covalent_binding_site	1	20	.	+	0	ID=annotation2051;Name=LuxR/HSL;Parent=BBa_R0063
>BBa_J329031
acctgtacgatcctacaggtgcttatgttaagtaattgtattcccagcgatacaatagtgtgacaaaaatccaatttat
tagaatcaaatgtcaatccattaccgttttaatgatatataacacgcaaaacttgcgacaaacaataggtaatactaga
gaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtaga
agcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcattt
atcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgc
taatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaac
aatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttcccta
ttcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttattttt
acatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaa
tcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaa
aaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctg
ccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctag
tgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttg
tttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacc
tgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatactaga
tgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatca
agtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgca
gaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgc
tgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgc
tgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacac
gctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttc
cttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatga
acagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctag
tgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttg
tttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacc
tgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatactaga
tgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaatt
ttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaacta
cctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaac
agcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaacta
caagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaa
gatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaaga
atggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaa
tactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatccc
aacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactataca
aaaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaagg
ctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcacc
ttcgggtgggcctttctgcgtttatatactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaat
gcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcatta
ggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatac
tagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatgga
aggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaa
ctgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagctt
acgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaa
cttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactg
cgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgt
acccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagt
taaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcc
cacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgat
agtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtttt
atctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata