LOCUS BBa_J329031 4026 bp DNA linear 30-Jun-2025 DEFINITION QS System with Strong RBS + Const. RFP Expression ACCESSION BBa_J329031 VERSION BBa_J329031.1 FEATURES Location/Qualifiers misc_feature 1..20 /label=LuxR/HSL promoter 115..122 /label=-10 promoter 89..94 /label=-35 misc_feature 128..128 /label=start misc_feature 130..149 /label=Putative LuxR/HSL misc_feature 1..151 /label=BBa_R0063 promoter 1..151 /label=BBa_R0063 misc_feature 164..167 /label=conserved RBS 160..171 /label=BBa_B0034 misc_feature 178..179 /label=prefix misc_feature 351..351 /label=T misc_feature 669..669 /label=A CDS 178..927 /label=luxR misc_feature 178..933 /label=BBa_C0062 misc_feature 934..958 /label=Help:Barcodes CDS 178..933 /label=BBa_C0062 stem_loop 978..1021 /label=stem_loop misc_feature 967..1046 /label=BBa_B0010 terminator 967..1046 /label=BBa_B0010 stem_loop 1062..1081 /label=T7 TE misc_feature 1088..1088 /label=stop polyA_site 1082..1095 /label=polya misc_feature 1055..1095 /label=BBa_B0012 terminator 1055..1095 /label=BBa_B0012 misc_feature 1104..1123 /label=LuxR/HSL promoter 1123..1128 /label=-35 promoter 1145..1150 /label=-10 misc_feature 1156..1156 /label=start misc_feature 1104..1158 /label=BBa_R0062 promoter 1104..1158 /label=BBa_R0062 misc_feature 1..1158 /label=BBa_F2621 misc_feature 1171..1174 /label=conserved RBS 1167..1178 /label=BBa_B0034 misc_feature 1764..1795 /label=LVA CDS 1185..1763 /label=luxI misc_feature 1185..1802 /label=BBa_C0061 misc_feature 1803..1827 /label=Help:Barcodes CDS 1185..1802 /label=BBa_C0061 misc_feature 1167..1827 /label=BBa_C0261 stem_loop 1847..1890 /label=stem_loop misc_feature 1836..1915 /label=BBa_B0010 terminator 1836..1915 /label=BBa_B0010 stem_loop 1931..1950 /label=T7 TE misc_feature 1957..1957 /label=stop polyA_site 1951..1964 /label=polya misc_feature 1924..1964 /label=BBa_B0012 terminator 1924..1964 /label=BBa_B0012 terminator 1836..1964 /label=BBa_B0015 misc_feature 1167..1964 /label=BBa_K082014 misc_feature 1..1964 /label=BBa_K546000 misc_feature 1973..1992 /label=LuxR/HSL promoter 1992..1997 /label=-35 promoter 2014..2019 /label=-10 misc_feature 2025..2025 /label=start misc_feature 1973..2027 /label=BBa_R0062 promoter 1973..2027 /label=BBa_R0062 misc_feature 2040..2043 /label=conserved RBS 2036..2047 /label=BBa_B0034 misc_feature 2054..2773 /label=BBa_E0040 misc_feature 2768..2806 /label=LVA misc_feature 2807..2812 /label=Double Stop misc_feature 2054..2056 /label=Start misc_feature 2054..2812 /label=BBa_J04031 stem_loop 2832..2875 /label=stem_loop misc_feature 2821..2900 /label=BBa_B0010 terminator 2821..2900 /label=BBa_B0010 stem_loop 2916..2935 /label=T7 TE misc_feature 2942..2942 /label=stop polyA_site 2936..2949 /label=polya misc_feature 2909..2949 /label=BBa_B0012 terminator 2909..2949 /label=BBa_B0012 misc_feature 2036..2949 /label=BBa_I763020 CDS 2958..3045 /label=end of LacI coding region (inactive) misc_feature 2958..3157 /label=BBa_R0010 misc_feature 3046..3083 /label=CAP binding site promoter 3094..3099 /label=-35 promoter 3118..3123 /label=-10 misc_feature 3123..3157 /label=LacI binding site misc_feature 3130..3130 /label=start promoter 2958..3157 /label=BBa_R0010 misc_feature 3170..3173 /label=conserved RBS 3166..3177 /label=BBa_B0034 CDS 3184..3858 /label=mrfp1 misc_feature 3865..3889 /label=Help:Barcodes CDS 3184..3864 /label=BBa_E1010 stem_loop 3909..3952 /label=stem_loop misc_feature 3898..3977 /label=BBa_B0010 terminator 3898..3977 /label=BBa_B0010 stem_loop 3993..4012 /label=T7 TE misc_feature 4019..4019 /label=stop polyA_site 4013..4026 /label=polya misc_feature 3986..4026 /label=BBa_B0012 terminator 3986..4026 /label=BBa_B0012 misc_feature 2958..4026 /label=BBa_J04450 ORIGIN 1 acctgtacga tcctacaggt gcttatgtta agtaattgta ttcccagcga tacaatagtg 61 tgacaaaaat ccaatttatt agaatcaaat gtcaatccat taccgtttta atgatatata 121 acacgcaaaa cttgcgacaa acaataggta atactagaga aagaggagaa atactagatg 181 aaaaacataa atgccgacga cacatacaga ataattaata aaattaaagc ttgtagaagc 241 aataatgata ttaatcaatg cttatctgat atgactaaaa tggtacattg tgaatattat 301 ttactcgcga tcatttatcc tcattctatg gttaaatctg atatttcaat cctagataat 361 taccctaaaa aatggaggca atattatgat gacgctaatt taataaaata tgatcctata 421 gtagattatt ctaactccaa tcattcacca attaattgga atatatttga aaacaatgct 481 gtaaataaaa aatctccaaa tgtaattaaa gaagcgaaaa catcaggtct tatcactggg 541 tttagtttcc ctattcatac ggctaacaat ggcttcggaa tgcttagttt tgcacattca 601 gaaaaagaca actatataga tagtttattt ttacatgcgt gtatgaacat accattaatt 661 gttccttctc tagttgataa ttatcgaaaa ataaatatag caaataataa atcaaacaac 721 gatttaacca aaagagaaaa agaatgttta gcgtgggcat gcgaaggaaa aagctcttgg 781 gatatttcaa aaatattagg ttgcagtgag cgtactgtca ctttccattt aaccaatgcg 841 caaatgaaac tcaatacaac aaaccgctgc caaagtattt ctaaagcaat tttaacagga 901 gcaattgatt gcccatactt taaaaattaa taacactgat agtgctagtg tagatcacta 961 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta 1021 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc 1081 ctttctgcgt ttatatacta gagacctgta ggatcgtaca ggtttacgca agaaaatggt 1141 ttgttatagt cgaataaata ctagagaaag aggagaaata ctagatgact ataatgataa 1201 aaaaatcgga ttttttggca attccatcgg aggagtataa aggtattcta agtcttcgtt 1261 atcaagtgtt taagcaaaga cttgagtggg acttagttgt agaaaataac cttgaatcag 1321 atgagtatga taactcaaat gcagaatata tttatgcttg tgatgatact gaaaatgtaa 1381 gtggatgctg gcgtttatta cctacaacag gtgattatat gctgaaaagt gtttttcctg 1441 aattgcttgg tcaacagagt gctcccaaag atcctaatat agtcgaatta agtcgttttg 1501 ctgtaggtaa aaatagctca aagataaata actctgctag tgaaattaca atgaaactat 1561 ttgaagctat atataaacac gctgttagtc aaggtattac agaatatgta acagtaacat 1621 caacagcaat agagcgattt ttaaagcgta ttaaagttcc ttgtcatcgt attggagaca 1681 aagaaattca tgtattaggt gatactaaat cggttgtatt gtctatgcct attaatgaac 1741 agtttaaaaa agcagtctta aatgctgcaa acgacgaaaa ctacgcttta gtagcttaat 1801 aactctgata gtgctagtgt agatctctac tagagccagg catcaaataa aacgaaaggc 1861 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta 1921 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag agacctgtag 1981 gatcgtacag gtttacgcaa gaaaatggtt tgttatagtc gaataaatac tagagaaaga 2041 ggagaaatac tagatgcgta aaggagaaga acttttcact ggagttgtcc caattcttgt 2101 tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga 2161 tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc 2221 atggccaaca cttgtcacta ctttcggtta tggtgttcaa tgctttgcga gatacccaga 2281 tcatatgaaa cagcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaaag 2341 aactatattt ttcaaagatg acgggaacta caagacacgt gctgaagtca agtttgaagg 2401 tgataccctt gttaatagaa tcgagttaaa aggtattgat tttaaagaag atggaaacat 2461 tcttggacac aaattggaat acaactataa ctcacacaat gtatacatca tggcagacaa 2521 acaaaagaat ggaatcaaag ttaacttcaa aattagacac aacattgaag atggaagcgt 2581 tcaactagca gaccattatc aacaaaatac tccaattggc gatggccctg tccttttacc 2641 agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg aaaagagaga 2701 ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca tggatgaact 2761 atacaaaagg cctgctgcaa acgacgaaaa ctacgcttta gtagcttaat aatactagag 2821 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt 2881 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct 2941 gcgtttatat actagagcaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta 3001 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa 3061 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat 3121 gttgtgtgga attgtgagcg gataacaatt tcacacatac tagagaaaga ggagaaatac 3181 tagatggctt cctccgaaga cgttatcaaa gagttcatgc gtttcaaagt tcgtatggaa 3241 ggttccgtta acggtcacga gttcgaaatc gaaggtgaag gtgaaggtcg tccgtacgaa 3301 ggtacccaga ccgctaaact gaaagttacc aaaggtggtc cgctgccgtt cgcttgggac 3361 atcctgtccc cgcagttcca gtacggttcc aaagcttacg ttaaacaccc ggctgacatc 3421 ccggactacc tgaaactgtc cttcccggaa ggtttcaaat gggaacgtgt tatgaacttc 3481 gaagacggtg gtgttgttac cgttacccag gactcctccc tgcaagacgg tgagttcatc 3541 tacaaagtta aactgcgtgg taccaacttc ccgtccgacg gtccggttat gcagaaaaaa 3601 accatgggtt gggaagcttc caccgaacgt atgtacccgg aagacggtgc tctgaaaggt 3661 gaaatcaaaa tgcgtctgaa actgaaagac ggtggtcact acgacgctga agttaaaacc 3721 acctacatgg ctaaaaaacc ggttcagctg ccgggtgctt acaaaaccga catcaaactg 3781 gacatcacct cccacaacga agactacacc atcgttgaac agtacgaacg tgctgaaggt 3841 cgtcactcca ccggtgctta ataacgctga tagtgctagt gtagatcgct actagagcca 3901 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt 3961 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg 4021 tttata //