##gff-version 3
##sequence-region BBa_J32024 1 3577
BBa_J32024	.	terminator	2509	2637	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_J32024	.	terminator	2509	2588	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_J32024	.	stem_loop	2520	2563	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J32024	.	engineered_region	2509	2588	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J32024	.	terminator	2597	2637	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_J32024	.	stem_loop	2604	2623	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J32024	.	polyA_site	2624	2637	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J32024	.	engineered_region	2597	2637	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J32024	.	stop_codon	2630	2630	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J32024	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_J32024	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J32024	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J32024	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J32024	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J32024	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J32024	.	ribosome_entry_site	2709	2720	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J32024	.	conserved_region	2713	2716	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J32024	.	terminator	1623	1751	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_J32024	.	terminator	1623	1702	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_J32024	.	stem_loop	1634	1677	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J32024	.	engineered_region	1623	1702	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J32024	.	terminator	1711	1751	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_J32024	.	stem_loop	1718	1737	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J32024	.	polyA_site	1738	1751	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J32024	.	engineered_region	1711	1751	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J32024	.	stop_codon	1744	1744	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J32024	.	promoter	2646	2699	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_J32024	.	operator	2671	2687	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_J32024	.	promoter	2688	2693	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_J32024	.	promoter	2665	2670	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_J32024	.	engineered_region	2646	2699	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_J32024	.	operator	2648	2664	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_J32024	.	ribosome_entry_site	870	881	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J32024	.	conserved_region	874	877	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J32024	.	CDS	81	836	.	+	0	ID=BBa_C0062;Name=BBa_C0062
BBa_J32024	.	engineered_region	81	82	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_J32024	.	engineered_tag	837	861	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_J32024	.	point_mutation	572	572	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_J32024	.	CDS	81	830	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_J32024	.	engineered_region	81	836	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_J32024	.	point_mutation	254	254	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_J32024	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J32024	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J32024	.	CDS	1841	2500	.	+	0	ID=BBa_J32021;Name=BBa_J32021
BBa_J32024	.	engineered_region	2727	3440	.	+	0	ID=BBa_J06504;Name=BBa_J06504
BBa_J32024	.	sequence_alteration	3078	3078	.	+	0	ID=annotation1585833;Name=C->T (removing PstI site);Parent=BBa_J06504
BBa_J32024	.	CDS	2727	3437	.	+	0	ID=annotation1585829;Name=mCherry;Parent=BBa_J06504
BBa_J32024	.	terminator	3449	3577	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_J32024	.	terminator	3449	3528	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_J32024	.	stem_loop	3460	3503	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J32024	.	engineered_region	3449	3528	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J32024	.	terminator	3537	3577	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_J32024	.	stem_loop	3544	3563	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J32024	.	polyA_site	3564	3577	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J32024	.	engineered_region	3537	3577	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J32024	.	stop_codon	3570	3570	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J32024	.	promoter	1760	1814	.	+	0	ID=BBa_R0062;Name=BBa_R0062
BBa_J32024	.	promoter	1779	1784	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_J32024	.	non_covalent_binding_site	1760	1779	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_J32024	.	promoter	1801	1806	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_J32024	.	start_codon	1812	1812	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_J32024	.	engineered_region	1760	1814	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_J32024	.	CDS	888	1614	.	+	0	ID=BBa_C0076;Name=BBa_C0076
BBa_J32024	.	engineered_tag	1590	1614	.	+	0	ID=annotation2214001;Name=Help:Barcodes;Parent=BBa_C0076
BBa_J32024	.	tag	1551	1583	.	+	0	ID=annotation306586;Name=LVA;Parent=BBa_C0076
BBa_J32024	.	CDS	888	1550	.	+	0	ID=annotation301035;Name=CinI;Parent=BBa_C0076
BBa_J32024	.	ribosome_entry_site	1823	1834	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J32024	.	conserved_region	1827	1830	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_J32024
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaat
caatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggtta
aatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatga
tcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaa
tctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatg
gcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacat
accattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaacc
aaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtg
agcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagc
aattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagag
aaagaggagaaatactagatgttcgttatcatccaggctcacgaataccagaaatacgctgctgttctggaccagatgt
tccgtctgcgtaaaaaagttttcgctgacaccctgtgctgggacgttccggttatcggtccgtacgaacgtgactccta
cgactccctggctccggcttacctggtttggtgcaacgactcccgtacccgtctgtacggtggtatgcgtctgatgccg
accaccggtccgaccctgctgtacgacgttttccgtgaaaccttcccggacgctgctgacctgatcgctccgggtatct
gggaaggtacccgtatgtgcatcgacgaagaagctatcgctaaagacttcccggaaatcgacgctggtcgtgctttctc
catgatgctgctggctctgtgcgaatgcgctctggaccacggtatccacaccatgatctccaactacgaaccgtacctg
aaacgtgtttacaaacgtgctggtgctgaagttgaagaactgggtcgtgctgacggttacggtaaatacccggtttgct
gcggtgctttcgaagtttccgaccgtgttctgcgtaaaatgcgtgctgctctgggtctgaccctgccgctgtacgttcg
tcacgttccggctcgttccgttgttacccagttcctggaaatggctgctgctgctaacgacgaaaactacgctctggtt
gcttaataaccctgatagtgctagtgtagatccctactagagccaggcatcaaataaaacgaaaggctcagtcgaaaga
ctgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcct
ttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatac
tagagaaagaggagaaatactagatggagaaaaaaatcactggatataccaccgttgatatatcccaatggcatcgtaa
agaacattttgaggcatttcagtcagttgctcaatgtacctataaccagaccgttcagctggatattacggccttttta
aagaccgtaaagaaaaataagcacaagttttatccggcctttattcacattcttgcccgcctgatgaatgctcatccgg
aatttcgtatggcaatgaaagacggtgagctggtgatatgggatagtgttcacccttgttacaccgttttccatgagca
aactgaaacgttttcatcgctctggagtgaataccacgacgatttccggcagtttctacacatatattcgcaagatgtg
gcgtgttacggtgaaaacctggcctatttccctaaagggtttattgagaatatgtttttcgtctcagccaatccctggg
tgagtttcaccagttttgatttaaacgtggccaatatggacaacttcttcgcccccgttttcaccatgggcaaatatta
tacgcaaggcgacaaggtgctgatgccgctggcgattcaggttcatcatgccgtttgtgatggcttccatgtcggcaga
atgcttaatgaattacaacagtactgcgatgagtggcagggcggggcgtaatactagagccaggcatcaaataaaacga
aaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggct
caccttcgggtgggcctttctgcgtttatatactagagaattgtgagcggataacaattgacattgtgagcggataaca
agatactgagcacatactagagaaagaggagaaatactagatggtgagcaagggcgaggaggataacatggccatcatc
aaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagg
gccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcct
gtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttc
cccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgc
aggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagac
catgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaag
ctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcct
acaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgaggg
ccgccactccaccggcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataaaacgaaaggctca
gtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcg
ggtgggcctttctgcgtttata