##gff-version 3
##sequence-region BBa_J3108 1 2237
BBa_J3108	.	terminator	1123	1202	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J3108	.	engineered_region	1123	1202	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J3108	.	stem_loop	1134	1177	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J3108	.	terminator	2109	2188	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J3108	.	engineered_region	2109	2188	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J3108	.	stem_loop	2120	2163	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J3108	.	promoter	1260	1459	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_J3108	.	non_covalent_binding_site	1348	1385	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_J3108	.	promoter	1420	1425	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_J3108	.	start_codon	1432	1432	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_J3108	.	engineered_region	1260	1459	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_J3108	.	non_covalent_binding_site	1425	1459	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_J3108	.	CDS	1260	1347	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_J3108	.	promoter	1396	1401	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_J3108	.	terminator	1211	1251	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J3108	.	polyA_site	1238	1251	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J3108	.	engineered_region	1211	1251	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J3108	.	stop_codon	1244	1244	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J3108	.	stem_loop	1218	1237	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J3108	.	CDS	1489	2100	.	+	0	ID=BBa_J31001;Name=BBa_J31001
BBa_J3108	.	stop_codon	2098	2100	.	+	0	ID=annotation1910567;Name=Stop codon;Parent=BBa_J31001
BBa_J3108	.	primer_binding_site	1489	1491	.	+	0	ID=annotation1910565;Name=Fwd primer J31001;Parent=BBa_J31001
BBa_J3108	.	tag	2059	2097	.	+	0	ID=annotation1910566;Name=LVA;Parent=BBa_J31001
BBa_J3108	.	primer_binding_site	2039	2100	.	+	0	ID=annotation1910570;Name=Rev primer J31001;Parent=BBa_J31001
BBa_J3108	.	CDS	1489	2058	.	+	0	ID=annotation1910564;Name=Hin;Parent=BBa_J31001
BBa_J3108	.	engineered_region	1	1114	.	+	0	ID=BBa_I13458;Name=BBa_I13458
BBa_J3108	.	terminator	2197	2237	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J3108	.	polyA_site	2224	2237	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J3108	.	engineered_region	2197	2237	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J3108	.	stop_codon	2230	2230	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J3108	.	stem_loop	2204	2223	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J3108	.	ribosome_entry_site	1468	1482	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_J3108	.	ribosome_entry_site	1475	1479	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_J3108	.	engineered_region	1468	1482	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_J3108	.	ribosome_entry_site	1468	1482	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
>BBa_J3108
ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcat
tttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtgg
tgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaa
aagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtga
tcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgcc
gcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttg
cccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccag
ttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgac
cgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcac
caccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataa
ccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcg
cttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactg
cgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaa
gccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacac
tttgctattactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtt
tgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagcaata
cgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggca
gtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtat
gttgtgtggaattgtgagcggataacaatttcacacatactagagattaaagaggagaaatactagatggctactattg
ggtatattcgggtgtcaacaattgaccaaaatatcgatttacagcgtaatgcgcttaccagtgcaaattgtgaccgcat
ttttgaggaccgtatcagtggcaagattgcaaaccgccccggcctgaaacgagcgttaaagtatgtaaataaaggcgat
actcttgtcgtctggaaattagacagactgggccgcagcgtgaaaaacctggtggcgttaatatcagaattacatgaac
gtggagctcacttccattctttaaccgatagtattgataccagtagcgcgatggggcgattcttttttcatgtaatgtc
agcactggccgagatggagcgagaattaattgtcgagcgaacccttgccggactggctgccgccagagcgcaaggacga
ctgggagggcgccctcgggcgatcaacagacatgaacaggaacagattagtcggctattagagaaaggccatcctcggc
agcaactagctattatttttggtattggcgtatctaccttatacagatattttccggcaagccgtataaaaaaacgaat
gaataggcctgctgcaaacgacgaaaactacgctttagtagcttaatactagagccaggcatcaaataaaacgaaaggc
tcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcacct
tcgggtgggcctttctgcgtttata