##gff-version 3 ##sequence-region BBa_J3107 1 2198 BBa_J3107 . promoter 1260 1459 . + 0 ID=BBa_R0010;Name=BBa_R0010 BBa_J3107 . engineered_region 1260 1459 . + 0 ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010 BBa_J3107 . CDS 1260 1347 . + 0 ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010 BBa_J3107 . non_covalent_binding_site 1425 1459 . + 0 ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010 BBa_J3107 . non_covalent_binding_site 1348 1385 . + 0 ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010 BBa_J3107 . promoter 1420 1425 . + 0 ID=annotation1961225;Name=-10;Parent=BBa_R0010 BBa_J3107 . promoter 1396 1401 . + 0 ID=annotation1961224;Name=-35;Parent=BBa_R0010 BBa_J3107 . start_codon 1432 1432 . + 0 ID=annotation1961227;Name=start;Parent=BBa_R0010 BBa_J3107 . terminator 1123 1202 . + 0 ID=BBa_B0010;Name=BBa_B0010 BBa_J3107 . engineered_region 1123 1202 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 BBa_J3107 . stem_loop 1134 1177 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 BBa_J3107 . CDS 1489 2061 . + 0 ID=BBa_J31000;Name=BBa_J31000 BBa_J3107 . CDS 1489 2061 . + 0 ID=annotation1884987;Name=Hin sequence;Parent=BBa_J31000 BBa_J3107 . primer_binding_site 1489 1491 . + 0 ID=annotation1910568;Name=Fwd primer J31001;Parent=BBa_J31000 BBa_J3107 . sequence_alteration 1558 1563 . + 0 ID=annotation1880459;Name=Former SpeI site;Parent=BBa_J31000 BBa_J3107 . sequence_alteration 1560 1560 . + 0 ID=annotation1880460;Name=Mutation to delete the SpeI site;Parent=BBa_J31000 BBa_J3107 . primer_binding_site 2039 2058 . + 0 ID=annotation1910569;Name=Rev primer J31001;Parent=BBa_J31000 BBa_J3107 . terminator 2070 2149 . + 0 ID=BBa_B0010;Name=BBa_B0010 BBa_J3107 . engineered_region 2070 2149 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 BBa_J3107 . stem_loop 2081 2124 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 BBa_J3107 . terminator 2158 2198 . + 0 ID=BBa_B0012;Name=BBa_B0012 BBa_J3107 . engineered_region 2158 2198 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_J3107 . stem_loop 2165 2184 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_J3107 . polyA_site 2185 2198 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_J3107 . stop_codon 2191 2191 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_J3107 . ribosome_entry_site 1468 1482 . + 0 ID=BBa_B0030;Name=BBa_B0030 BBa_J3107 . engineered_region 1468 1482 . + 0 ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030 BBa_J3107 . ribosome_entry_site 1468 1482 . + 0 ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030 BBa_J3107 . ribosome_entry_site 1475 1479 . + 0 ID=annotation1702;Name=RBS;Parent=BBa_B0030 BBa_J3107 . engineered_region 1 1114 . + 0 ID=BBa_I13458;Name=BBa_I13458 BBa_J3107 . terminator 1211 1251 . + 0 ID=BBa_B0012;Name=BBa_B0012 BBa_J3107 . engineered_region 1211 1251 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_J3107 . stem_loop 1218 1237 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_J3107 . polyA_site 1238 1251 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_J3107 . stop_codon 1244 1244 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 >BBa_J3107 ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcat tttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtgg tgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaa aagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtga tcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgcc gcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttg cccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccag ttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgac cgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcac caccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataa ccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcg cttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactg cgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaa gccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacac tttgctattactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtt tgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagcaata cgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggca gtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtat gttgtgtggaattgtgagcggataacaatttcacacatactagagattaaagaggagaaatactagatggctactattg ggtatattcgggtgtcaacaattgaccaaaatatcgatttacagcgtaatgcgcttaccagtgcaaattgtgaccgcat ttttgaagaccgtatcagtggcaagattgcaaaccgccccggcctgaaacgggcgttaaagtatgtaaataaaggcgat actcttgtcgtctggaaattagacagactgggccgtagcgtgaaaaatctggtggcgttaatatcagaattacatgaac gtggagctcacttccattctttaaccgatagtattgataccagtagcgcgatggggcgattcttttttcatgtaatgtc agcactggccgagatggagcgagaattaatcgtcgagcgaacccttgccggactggctgccgccagagcgcaaggacga ctgggagggcgccctcgggcgatcaacaaacatgaacaggaacagattagtcggctattagagaaaggccatcctcggc agcaattagctattatttttggtattggcgtatccaccttatacagatactttccggcaagcagtataaaaaaacgaat gaattaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgttt gtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata