##gff-version 3
##sequence-region BBa_J29018 1 4210
BBa_J29018	.	engineered_region	1	1371	.	+	0	ID=BBa_Q04121;Name=BBa_Q04121
BBa_J29018	.	terminator	1180	1308	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_Q04121
BBa_J29018	.	terminator	1180	1259	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_J29018	.	engineered_region	1180	1259	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J29018	.	stem_loop	1191	1234	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J29018	.	terminator	1268	1308	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_J29018	.	engineered_region	1268	1308	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J29018	.	stop_codon	1301	1301	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J29018	.	stem_loop	1275	1294	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J29018	.	polyA_site	1295	1308	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J29018	.	promoter	1317	1370	.	+	0	ID=BBa_R0011;Name=BBa_R0011;Parent=BBa_Q04121
BBa_J29018	.	promoter	1359	1364	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_J29018	.	promoter	1336	1341	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_J29018	.	operator	1342	1358	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_J29018	.	engineered_region	1317	1370	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_J29018	.	operator	1319	1335	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_J29018	.	CDS	19	1146	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_Q04121
BBa_J29018	.	engineered_tag	1147	1171	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_J29018	.	engineered_region	19	1146	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_J29018	.	CDS	19	1146	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_J29018	.	tag	1108	1146	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_J29018	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_Q04121
BBa_J29018	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J29018	.	engineered_region	1548	2564	.	+	0	ID=BBa_J29004;Name=BBa_J29004
BBa_J29018	.	terminator	2470	2510	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J29004
BBa_J29018	.	engineered_region	2470	2510	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J29018	.	stop_codon	2503	2503	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J29018	.	stem_loop	2477	2496	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J29018	.	polyA_site	2497	2510	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J29018	.	CDS	1568	2461	.	+	0	ID=BBa_J29001;Name=BBa_J29001;Parent=BBa_J29004
BBa_J29018	.	stop_codon	2456	2461	.	+	0	ID=annotation1892586;Name=double stop codon;Parent=BBa_J29001
BBa_J29018	.	CDS	1568	2461	.	+	0	ID=annotation1892590;Name=crtB coding sequence;Parent=BBa_J29001
BBa_J29018	.	ribosome_entry_site	1548	1561	.	+	0	ID=BBa_B0031;Name=BBa_B0031;Parent=BBa_J29004
BBa_J29018	.	conserved_region	1554	1557	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
BBa_J29018	.	terminator	2519	2564	.	+	0	ID=BBa_B0011;Name=BBa_B0011;Parent=BBa_J29004
BBa_J29018	.	stem_loop	2531	2553	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_J29018	.	engineered_region	2519	2564	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_J29018	.	engineered_region	2573	4210	.	+	0	ID=BBa_J29005;Name=BBa_J29005
BBa_J29018	.	terminator	4082	4161	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J29005
BBa_J29018	.	engineered_region	4082	4161	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J29018	.	stem_loop	4093	4136	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J29018	.	ribosome_entry_site	2573	2585	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_J29005
BBa_J29018	.	ribosome_entry_site	2579	2582	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_J29018	.	ribosome_entry_site	2573	2585	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_J29018	.	engineered_region	2573	2585	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_J29018	.	CDS	2592	4073	.	+	0	ID=BBa_J29002;Name=BBa_J29002;Parent=BBa_J29005
BBa_J29018	.	CDS	2592	4073	.	+	0	ID=annotation1892591;Name=CrtI coding sequence;Parent=BBa_J29002
BBa_J29018	.	stop_codon	4068	4073	.	+	0	ID=annotation1892588;Name=Double stop codon;Parent=BBa_J29002
BBa_J29018	.	terminator	4170	4210	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J29005
BBa_J29018	.	engineered_region	4170	4210	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J29018	.	stop_codon	4203	4203	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J29018	.	stem_loop	4177	4196	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J29018	.	polyA_site	4197	4210	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J29018	.	engineered_region	1380	1539	.	+	0	ID=BBa_J29003;Name=BBa_J29003
BBa_J29018	.	terminator	1411	1539	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_J29003
BBa_J29018	.	terminator	1411	1490	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_J29018	.	engineered_region	1411	1490	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J29018	.	stem_loop	1422	1465	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J29018	.	terminator	1499	1539	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_J29018	.	engineered_region	1499	1539	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J29018	.	stop_codon	1532	1532	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J29018	.	stem_loop	1506	1525	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J29018	.	polyA_site	1526	1539	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J29018	.	ribosome_entry_site	1380	1394	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_J29003
BBa_J29018	.	engineered_region	1380	1394	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_J29018	.	ribosome_entry_site	1387	1391	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_J29018	.	ribosome_entry_site	1380	1394	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
>BBa_J29018
aaagaggagaaatactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatc
agaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcgga
gctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagt
ctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgt
cgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgat
cattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgat
gtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcg
cattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggca
taaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaa
accatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgc
gcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatg
ttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactc
tctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaata
cgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggca
ggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggc
atcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctacta
gagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaattgtgagcggataacaattgacatt
gtgagcggataacaagatactgagcacatactagagattaaagaggagaaatactagagtactagagccaggcatcaaa
taaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtca
cactggctcaccttcgggtgggcctttctgcgtttatatactagagtcacacaggaaacctactagatggcagttggct
cgaaaagttttgcgacagcctcaaagttatttgatgcaaaaacccggcgcagcgtactgatgctctacgcctggtgccg
ccattgtgacgatgttattgacgatcagacgctgggctttcaggcccggcagcctgccttacaaacgcccgaacaacgt
ctgatgcaacttgagatgaaaacgcgccaggcctatgcaggatcgcagatgcacgaaccggcgtttgcggcttttcagg
aagtggctatggctcatgatatcgccccggcttacgcgtttgatcatctggaaggcttcgccatggatgtacgcgaagc
gcaatacagccaactggatgatacgctgcgctattgctatcacgttgcaggcgttgtcggcttgatgatggcgcaaatc
atgggcgtacgggataacgccacgctggaccgcgcctgtgaccttgggctggcatttcagttgaccaatattgctcgcg
atattgtggacgatgcgcatgcgggccgctgttatctgccggcaagctggctggagcatgaaggtctgaacaaagagaa
ttatgcggcacctgaaaaccgtcaggcgctgagccgtatcgcccgtcgtttggtgcaggaagcagaaccttactatttg
tctgccacagccggcctggcagggttgcccctgcgttccgcctgggcaatcgctacggcgaagcaggtttaccggaaaa
taggtgtcaaagttgaacaggccggtcagcaagcctgggatcagcggcagtcaacgaccacgcccgaaaaattaacgct
gctgctggccgcctctggtcaggcccttacttcccggatgcgggctcatcctccccgccctgcgcatctctggcagcgc
ccgctctaataatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatata
aaaagccagattattaatccggcttttttattattttactagagtcacacaggaaagtactagatgaaaccaactacgg
taattggtgcaggcttcggtggcctggcactggcaattcgtctacaagctgcggggatccccgtcttactgcttgaaca
acgtgataaacccggcggtcgggcttatgtctacgaggatcaggggtttacctttgatgcaggcccgacggttatcacc
gatcccagtgccattgaagaactgtttgcactggcaggaaaacagttaaaagagtatgtcgaactgctgccggttacgc
cgttttaccgcctgtgttgggagtcagggaaggtctttaattacgataacgatcaaacccggctcgaagcgcagattca
gcagtttaatccccgcgatgtcgaaggttatcgtcagtttctggactattcacgcgcggtgtttaaagaaggctatcta
aagctcggtactgtcccttttttatcgttcagagacatgcttcgcgccgcacctcaactggcgaaactccaagcatgga
gaagcgtttacagtaaggttgccagttacatcgaagatgaacatctgcgccaggcgttttctttccactcgctgttggt
gggcggcaatcccttcgccacctcatccatttatacgttgatacacgcgctggagcgtgagtggggcgtctggtttccg
cgtggcggcaccggcgcattagttcaggggatgataaagctgtttcaggatctgggtggcgaagtcgtgttaaacgcca
gagtcagccatatggaaacgacaggaaacaagattgaagccgtgcatttagaggacggtcgcaggttcctgacgcaagc
cgtcgcgtcaaatgcagatgtggttcatacctatcgcgacctgttaagccagcaccctgccgcggttaagcagtccaac
aaactccaaactaagcgcatgagtaactctctgtctgtgctctattttggtttgaatcaccatcatgatcagctcgcgc
atcacacggtttgtttcggcccgcgttaccgcgagctgattgacgaagtttttaatcatgatggcctcgcagaggactt
ctcactttatctgcacgcgccctgtgtcacggattcgtcactggcgcctgaaggttgcggcagttactatgtgttggcg
ccggtgccgcatttaggcaccgcgaacctcgactggacggttgaggggccaaaactacgcgaccgtatttttgcgtacc
ttgagcagcattacatgcctggcttacggagtcagctggtcacgcaccggatgtttacgccgtttgattttcgcgacca
gcttaatgcctatcatggctcagccttttctgtggagcccgttcttacccagagcgcctggtttcggccgcataaccgc
gataaaaccattactaatctctacctggtcggcgcaggcacgcatcccggcgcaggcattcctggcgtcatcggctcgg
caaaagcgacagcaggtttgatgctggaggatctgatttaataatactagagccaggcatcaaataaaacgaaaggctc
agtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttc
gggtgggcctttctgcgtttata