##gff-version 3
##sequence-region BBa_J29013 1 3902
BBa_J29013	.	CDS	1	720	.	+	0	ID=BBa_E0040;Name=BBa_E0040
BBa_J29013	.	CDS	1	720	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_J29013	.	CDS	747	1874	.	+	0	ID=BBa_C0012;Name=BBa_C0012
BBa_J29013	.	engineered_tag	1875	1899	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_J29013	.	engineered_region	747	1874	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_J29013	.	CDS	747	1874	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_J29013	.	tag	1836	1874	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_J29013	.	CDS	2284	3765	.	+	0	ID=BBa_J29002;Name=BBa_J29002
BBa_J29013	.	CDS	2284	3765	.	+	0	ID=annotation1892591;Name=CrtI coding sequence;Parent=BBa_J29002
BBa_J29013	.	stop_codon	3760	3765	.	+	0	ID=annotation1892588;Name=Double stop codon;Parent=BBa_J29002
BBa_J29013	.	ribosome_entry_site	729	740	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J29013	.	conserved_region	733	736	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J29013	.	terminator	1996	2036	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J29013	.	engineered_region	1996	2036	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J29013	.	stop_codon	2029	2029	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J29013	.	stem_loop	2003	2022	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J29013	.	polyA_site	2023	2036	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J29013	.	ribosome_entry_site	2265	2277	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_J29013	.	ribosome_entry_site	2271	2274	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_J29013	.	ribosome_entry_site	2265	2277	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_J29013	.	engineered_region	2265	2277	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_J29013	.	promoter	2108	2256	.	+	0	ID=BBa_R0080;Name=BBa_R0080
BBa_J29013	.	operator	2113	2151	.	+	0	ID=annotation301457;Name=araO1;Parent=BBa_R0080
BBa_J29013	.	promoter	2220	2225	.	+	0	ID=annotation308601;Name=-35;Parent=BBa_R0080
BBa_J29013	.	non_covalent_binding_site	2150	2179	.	+	0	ID=annotation301458;Name=c-amp1;Parent=BBa_R0080
BBa_J29013	.	non_covalent_binding_site	2111	2136	.	+	0	ID=annotation301456;Name=c-amp2;Parent=BBa_R0080
BBa_J29013	.	non_covalent_binding_site	2180	2208	.	+	0	ID=annotation301462;Name=ara1 and ara2;Parent=BBa_R0080
BBa_J29013	.	promoter	2243	2248	.	+	0	ID=annotation308602;Name=-10;Parent=BBa_R0080
BBa_J29013	.	terminator	3862	3902	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J29013	.	engineered_region	3862	3902	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J29013	.	stop_codon	3895	3895	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J29013	.	stem_loop	3869	3888	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J29013	.	polyA_site	3889	3902	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J29013	.	terminator	1908	1987	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J29013	.	engineered_region	1908	1987	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J29013	.	stem_loop	1919	1962	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J29013	.	terminator	3774	3853	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J29013	.	engineered_region	3774	3853	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J29013	.	stem_loop	3785	3828	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J29013	.	promoter	2045	2098	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_J29013	.	promoter	2087	2092	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_J29013	.	promoter	2064	2069	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_J29013	.	operator	2070	2086	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_J29013	.	engineered_region	2045	2098	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_J29013	.	operator	2047	2063	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
>BBa_J29013
atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaat
tttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaact
acctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaa
cagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaact
acaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaaga
agatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaag
aatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaa
atactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcc
caacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatac
aaataataatactagagaaagaggagaaatactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagta
tgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtg
gaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattg
gcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactggg
tgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaa
cgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttc
cggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactggg
cgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctg
cgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgcca
tgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagat
ggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgat
accgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtgg
accgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaac
caccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcc
cgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagat
ctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcg
gtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaattgtgagc
ggataacaattgacattgtgagcggataacaagatactgagcacatactagaggcgtaacaaaagtgtctataatcacg
gcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcgga
tcctacctgacgctttttatcgcaactctctactgtttctccattactagagtcacacaggaaagtactagatgaaacc
aactacggtaattggtgcaggcttcggtggcctggcactggcaattcgtctacaagctgcggggatccccgtcttactg
cttgaacaacgtgataaacccggcggtcgggcttatgtctacgaggatcaggggtttacctttgatgcaggcccgacgg
ttatcaccgatcccagtgccattgaagaactgtttgcactggcaggaaaacagttaaaagagtatgtcgaactgctgcc
ggttacgccgttttaccgcctgtgttgggagtcagggaaggtctttaattacgataacgatcaaacccggctcgaagcg
cagattcagcagtttaatccccgcgatgtcgaaggttatcgtcagtttctggactattcacgcgcggtgtttaaagaag
gctatctaaagctcggtactgtcccttttttatcgttcagagacatgcttcgcgccgcacctcaactggcgaaactcca
agcatggagaagcgtttacagtaaggttgccagttacatcgaagatgaacatctgcgccaggcgttttctttccactcg
ctgttggtgggcggcaatcccttcgccacctcatccatttatacgttgatacacgcgctggagcgtgagtggggcgtct
ggtttccgcgtggcggcaccggcgcattagttcaggggatgataaagctgtttcaggatctgggtggcgaagtcgtgtt
aaacgccagagtcagccatatggaaacgacaggaaacaagattgaagccgtgcatttagaggacggtcgcaggttcctg
acgcaagccgtcgcgtcaaatgcagatgtggttcatacctatcgcgacctgttaagccagcaccctgccgcggttaagc
agtccaacaaactccaaactaagcgcatgagtaactctctgtctgtgctctattttggtttgaatcaccatcatgatca
gctcgcgcatcacacggtttgtttcggcccgcgttaccgcgagctgattgacgaagtttttaatcatgatggcctcgca
gaggacttctcactttatctgcacgcgccctgtgtcacggattcgtcactggcgcctgaaggttgcggcagttactatg
tgttggcgccggtgccgcatttaggcaccgcgaacctcgactggacggttgaggggccaaaactacgcgaccgtatttt
tgcgtaccttgagcagcattacatgcctggcttacggagtcagctggtcacgcaccggatgtttacgccgtttgatttt
cgcgaccagcttaatgcctatcatggctcagccttttctgtggagcccgttcttacccagagcgcctggtttcggccgc
ataaccgcgataaaaccattactaatctctacctggtcggcgcaggcacgcatcccggcgcaggcattcctggcgtcat
cggctcggcaaaagcgacagcaggtttgatgctggaggatctgatttaataatactagagccaggcatcaaataaaacg
aaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggc
tcaccttcgggtgggcctttctgcgtttata