##gff-version 3
##sequence-region BBa_J28005 1 2642
BBa_J28005	.	engineered_region	166	2642	.	+	0	ID=BBa_S03554;Name=BBa_S03554
BBa_J28005	.	engineered_region	166	877	.	+	0	ID=BBa_S0104;Name=BBa_S0104;Parent=BBa_S03554
BBa_J28005	.	CDS	184	852	.	+	0	ID=BBa_C0052;Name=BBa_C0052;Parent=BBa_S0104
BBa_J28005	.	engineered_region	184	852	.	+	0	ID=annotation7035;Name=BBa_C0052;Parent=BBa_C0052
BBa_J28005	.	tag	814	852	.	+	0	ID=annotation1745;Name=LVA;Parent=BBa_C0052
BBa_J28005	.	CDS	184	852	.	+	0	ID=annotation1743;Name=cI 434;Parent=BBa_C0052
BBa_J28005	.	engineered_tag	853	877	.	+	0	ID=annotation2213992;Name=Help:Barcodes;Parent=BBa_C0052
BBa_J28005	.	ribosome_entry_site	166	177	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S0104
BBa_J28005	.	conserved_region	170	173	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J28005	.	engineered_region	886	2642	.	+	0	ID=BBa_S03545;Name=BBa_S03545;Parent=BBa_S03554
BBa_J28005	.	engineered_region	886	1717	.	+	0	ID=BBa_C0260;Name=BBa_C0260;Parent=BBa_S03545
BBa_J28005	.	ribosome_entry_site	886	897	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_C0260
BBa_J28005	.	conserved_region	890	893	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J28005	.	CDS	904	1692	.	+	0	ID=BBa_C0060;Name=BBa_C0060;Parent=BBa_C0260
BBa_J28005	.	CDS	904	1653	.	+	0	ID=annotation1757;Name=aiiA;Parent=BBa_C0060
BBa_J28005	.	start_codon	904	906	.	+	0	ID=annotation1754;Name=start;Parent=BBa_C0060
BBa_J28005	.	engineered_region	904	1692	.	+	0	ID=annotation7037;Name=BBa_C0060;Parent=BBa_C0060
BBa_J28005	.	engineered_tag	1693	1717	.	+	0	ID=annotation2213987;Name=Help:Barcodes;Parent=BBa_C0060
BBa_J28005	.	tag	1654	1686	.	+	0	ID=annotation1756;Name=LVA;Parent=BBa_C0060
BBa_J28005	.	stop_codon	1687	1692	.	+	0	ID=annotation1755;Name=2;Parent=BBa_C0060
BBa_J28005	.	engineered_region	1726	2642	.	+	0	ID=BBa_E0432;Name=BBa_E0432;Parent=BBa_S03545
BBa_J28005	.	terminator	2514	2593	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0432
BBa_J28005	.	stem_loop	2525	2568	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J28005	.	engineered_region	2514	2593	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J28005	.	ribosome_entry_site	1726	1737	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_E0432
BBa_J28005	.	conserved_region	1730	1733	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J28005	.	engineered_region	1744	2505	.	+	0	ID=BBa_E0032;Name=BBa_E0032;Parent=BBa_E0432
BBa_J28005	.	CDS	1744	2505	.	+	0	ID=annotation2156;Name=YFP (LVA);Parent=BBa_E0032
BBa_J28005	.	sequence_alteration	1985	1985	.	+	0	ID=annotation2161;Name=A (Q->L);Parent=BBa_E0032
BBa_J28005	.	engineered_region	1744	2505	.	+	0	ID=annotation7041;Name=BBa_E0032;Parent=BBa_E0032
BBa_J28005	.	stop_codon	2500	2505	.	+	0	ID=annotation2159;Name=2;Parent=BBa_E0032
BBa_J28005	.	tag	2461	2499	.	+	0	ID=annotation2160;Name=SsrA;Parent=BBa_E0032
BBa_J28005	.	terminator	2602	2642	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0432
BBa_J28005	.	stem_loop	2609	2628	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J28005	.	stop_codon	2635	2635	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J28005	.	polyA_site	2629	2642	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J28005	.	engineered_region	2602	2642	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J28005	.	promoter	1	157	.	+	0	ID=BBa_R0079;Name=BBa_R0079
BBa_J28005	.	non_covalent_binding_site	46	63	.	+	0	ID=annotation300985;Name=OP2;Parent=BBa_R0079
BBa_J28005	.	promoter	117	122	.	+	0	ID=annotation318495;Name=-35;Parent=BBa_R0079
BBa_J28005	.	promoter	140	145	.	+	0	ID=annotation318496;Name=-10;Parent=BBa_R0079
BBa_J28005	.	non_covalent_binding_site	106	125	.	+	0	ID=annotation300992;Name=OP1;Parent=BBa_R0079
>BBa_J28005
gcccctcgctgagcgcgtcccggagctgggggcaacctagctgccacctgcttttctgctagctattccagcgaaaaca
tacagatttccggcgaaatcaaggctacctgccagttctggcaggtttggccgcgggttctttttggtacacgaaagct
actagagaaagaggagaaatactagatgagtatttcttccagggtaaaaagcaaaagaatccagcttggacttaaccag
gctgaacttgctcaaaaggtggggactacccagcagtctatagagcagctcgaaaacggtaaaactaagcgaccacgct
ttttaccagaacttgcgtcagctcttggcgtaagtgttgactggctgctcaatggcacctctgattcgaatgttagatt
tgttgggcacgttgagcccaaagggaaatatccattgattagcatggttagagctggttcgtggtgtgaagcttgtgaa
ccctacgatatcaaggacattgatgaatggtatgacagtgacgttaacttattaggcaatggattctggctgaaggttg
aaggtgattccatgacctcacctgtaggtcaaagcatccctgaaggtcatatggtgttagtagatactggacgggagcc
agtgaatggaagccttgttgtagccaaactgactgacgcgaacgaagcaacattcaagaaactggtcatagatggcggt
cagaagtacctgaaaggcctgaatccttcatggcctatgactcctatcaacggaaactgcaagattatcggtgttgtcg
tggaagcgagggtaaaattcgtagctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtg
tagatctctactagagaaagaggagaaatactagatgacagtaaagaagctttatttcgtcccagcaggtcgttgtatg
ttggatcattcgtctgttaatagtacattaacaccaggagaattattagacttaccggtttggtgttatcttttggaga
ctgaagaaggacctattttagtagatacaggtatgccagaaagtgcagttaataatgaaggtctttttaacggtacatt
tgtcgaagggcaggttttaccgaaaatgactgaagaagatagaatcgtgaatattttaaaacgggttggttatgagccg
gaagaccttctttatattattagttctcacttgcattttgatcatgcaggaggaaatggcgcttttataaatacaccaa
tcattgtacagcgtgctgaatatgaggcggcgcagcatagcgaagaatatttgaaagaatgtatattgccgaatttaaa
ctacaaaatcattgaaggtgattatgaagtcgtaccaggagttcaattattgcatacaccaggccatactccagggcat
caatcgctattaattgagacagaaaaatccggtcctgtattattaacgattgatgcatcgtatacgaaagagaattttg
aaaatgaagtgccatttgcgggatttgattcagaattagctttatcttcaattaaacgtttaaaagaagtggtgatgaa
agagaagccgattgttttctttggacatgatatagagcaggaaaggggatgtaaagtgttccctgaatatatagctgca
aacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagaaagaggagaaat
actagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacgg
ccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccacc
ggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgacc
acatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacga
cggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgac
ttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgaca
agcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccacta
ccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagc
aaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacg
agctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataa
aacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacac
tggctcaccttcgggtgggcctttctgcgtttata