##gff-version 3
##sequence-region BBa_J24706 1 1590
BBa_J24706	.	engineered_region	1	704	.	+	0	ID=BBa_J24687;Name=BBa_J24687
BBa_J24706	.	CDS	20	704	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_J24687
BBa_J24706	.	tag	640	673	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_J24706	.	engineered_tag	680	704	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_J24706	.	CDS	23	639	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_J24706	.	ribosome_entry_site	1	13	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_J24687
BBa_J24706	.	ribosome_entry_site	7	10	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_J24706	.	ribosome_entry_site	1	13	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_J24706	.	engineered_region	1	13	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_J24706	.	engineered_region	713	1590	.	+	0	ID=BBa_J24702;Name=BBa_J24702
BBa_J24706	.	engineered_region	731	1453	.	+	0	ID=BBa_J06505;Name=BBa_J06505;Parent=BBa_J24702
BBa_J24706	.	CDS	731	1438	.	+	0	ID=annotation1585879;Name=mCherry;Parent=BBa_J06505
BBa_J24706	.	sequence_alteration	1082	1082	.	+	0	ID=annotation1585880;Name=C->T (removing PstI site);Parent=BBa_J06505
BBa_J24706	.	tag	1439	1447	.	+	0	ID=annotation1585887;Name=LVA;Parent=BBa_J06505
BBa_J24706	.	terminator	1462	1541	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J24702
BBa_J24706	.	stem_loop	1473	1516	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J24706	.	engineered_region	1462	1541	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J24706	.	ribosome_entry_site	713	724	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J24702
BBa_J24706	.	conserved_region	717	720	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J24706	.	terminator	1550	1590	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J24702
BBa_J24706	.	engineered_region	1550	1590	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J24706	.	stop_codon	1583	1583	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J24706	.	polyA_site	1577	1590	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J24706	.	stem_loop	1557	1576	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
>BBa_J24706
tcacacaggaaagtactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtc
ggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaa
ataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaagggga
aagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagta
catttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggttttt
cactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatca
agtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgat
caccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaa
gtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactaga
gaaagaggagaaatactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaag
gtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcaccc
agaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacgg
ctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggag
cgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctaca
aggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctc
cgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactac
gacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttgg
acatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcat
ggacgagctgtacaagttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactg
ggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttc
tgcgtttata