##gff-version 3
##sequence-region BBa_J21000 1 2916
BBa_J21000	.	ribosome_entry_site	1013	1024	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J21000	.	conserved_region	1017	1020	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J21000	.	terminator	1889	1929	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J21000	.	engineered_region	1889	1929	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J21000	.	stop_codon	1922	1922	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J21000	.	polyA_site	1916	1929	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J21000	.	stem_loop	1896	1915	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J21000	.	CDS	85	804	.	+	0	ID=BBa_E0040;Name=BBa_E0040
BBa_J21000	.	CDS	85	804	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_J21000	.	promoter	1938	1991	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_J21000	.	promoter	1980	1985	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J21000	.	non_covalent_binding_site	1963	1981	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J21000	.	promoter	1957	1962	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J21000	.	engineered_region	1938	1991	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J21000	.	non_covalent_binding_site	1938	1956	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J21000	.	ribosome_entry_site	2000	2011	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J21000	.	conserved_region	2004	2007	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J21000	.	terminator	2788	2867	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J21000	.	engineered_region	2788	2867	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J21000	.	stem_loop	2799	2842	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J21000	.	engineered_region	2018	2779	.	+	0	ID=BBa_E0022;Name=BBa_E0022
BBa_J21000	.	stop_codon	2774	2779	.	+	0	ID=annotation2154;Name=2;Parent=BBa_E0022
BBa_J21000	.	CDS	2018	2779	.	+	0	ID=annotation2150;Name=CFP (LVA);Parent=BBa_E0022
BBa_J21000	.	tag	2736	2773	.	+	0	ID=annotation2153;Name=SsrA;Parent=BBa_E0022
BBa_J21000	.	engineered_region	2018	2779	.	+	0	ID=annotation7040;Name=BBa_E0022;Parent=BBa_E0022
BBa_J21000	.	point_mutation	2086	2086	.	+	0	ID=annotation2155;Name=A;Parent=BBa_E0022
BBa_J21000	.	promoter	950	1003	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_J21000	.	operator	952	968	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_J21000	.	promoter	992	997	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_J21000	.	engineered_region	950	1003	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_J21000	.	operator	975	991	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_J21000	.	promoter	969	974	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_J21000	.	promoter	1	55	.	+	0	ID=BBa_R0062;Name=BBa_R0062
BBa_J21000	.	promoter	20	25	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_J21000	.	promoter	42	47	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_J21000	.	start_codon	53	53	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_J21000	.	engineered_region	1	55	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_J21000	.	non_covalent_binding_site	1	20	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_J21000	.	terminator	813	892	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J21000	.	engineered_region	813	892	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J21000	.	stem_loop	824	867	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J21000	.	engineered_region	1031	1792	.	+	0	ID=BBa_E0032;Name=BBa_E0032
BBa_J21000	.	stop_codon	1787	1792	.	+	0	ID=annotation2159;Name=2;Parent=BBa_E0032
BBa_J21000	.	tag	1748	1786	.	+	0	ID=annotation2160;Name=SsrA;Parent=BBa_E0032
BBa_J21000	.	CDS	1031	1792	.	+	0	ID=annotation2156;Name=YFP (LVA);Parent=BBa_E0032
BBa_J21000	.	engineered_region	1031	1792	.	+	0	ID=annotation7041;Name=BBa_E0032;Parent=BBa_E0032
BBa_J21000	.	sequence_alteration	1272	1272	.	+	0	ID=annotation2161;Name=A (Q->L);Parent=BBa_E0032
BBa_J21000	.	ribosome_entry_site	64	78	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_J21000	.	ribosome_entry_site	64	78	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_J21000	.	engineered_region	64	78	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_J21000	.	ribosome_entry_site	71	75	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_J21000	.	terminator	1801	1880	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J21000	.	engineered_region	1801	1880	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J21000	.	stem_loop	1812	1855	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J21000	.	terminator	2876	2916	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J21000	.	engineered_region	2876	2916	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J21000	.	stop_codon	2909	2909	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J21000	.	polyA_site	2903	2916	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J21000	.	stem_loop	2883	2902	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J21000	.	terminator	901	941	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J21000	.	engineered_region	901	941	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J21000	.	stop_codon	934	934	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J21000	.	polyA_site	928	941	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J21000	.	stem_loop	908	927	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
>BBa_J21000
acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagattaaagaggagaaat
actagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggca
caaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactgga
aaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcata
tgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgg
gaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgatttt
aaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaac
aaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatca
acaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaa
gatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaac
tatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatct
gttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactaga
gaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatac
tagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggcc
acaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccgg
caagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccac
atgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacg
gcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgactt
caaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaag
cagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactacc
agcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaa
agaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgag
ctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaa
cgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactg
gctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtga
tagagatactgagcactactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtgg
tgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccac
ctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctg
acctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaag
gctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcga
caccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtac
aactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccaca
acatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcc
cgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggag
ttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctt
tagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatct
gttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata